Varietal differences of grain sorghum in the quality of spare grain polymers

It is determined the number of drops in 34 samples of grain sorghum, as well as in wheat samples with 20% of the sample weight replaced by sorghum material. The attack capacity of grain sorghum starch was assessed by changing the number of drops when provoked by wheat amylase-active material. The state of the carbohydrate-amylase complex was evaluated by the value of the fall number, by its change when exposed to active amylases, and by the change in the fall number of wheat material when it was partially replaced with sorghum material. The state of the protein-proteinase complex was evaluated by the degree and nature of integration of biopolymers of sorghum grain with wheat gluten from grain from industrial sowing, through washing of gluten with the addition of ground sorghum grain and changes in the rheological properties of gluten. On the varietal material of grain sorghum, significant differences in the properties of spare grain polymers were revealed. Sorghum varieties with the potential to improve the properties of wheat flour in mixtures were identified. According to the complex of features, the most universal variety of grain sorghum for mixing with wheat material can be recognized as Cream. Its material did not respond to amylase provocation, moderately improved the low quality of wheat, demonstrated integration with gluten of various qualities, moderately strengthened weak and weakened strong gluten.

Dietary Wheat Amylase Trypsin Inhibitors Impact Alzheimer’s Disease Pathology in 5xFAD Model Mice

Wheat amylase trypsin inhibitors (ATIs) represent a common dietary protein component of gluten-containing cereals (wheat, rye, and barley). They act as toll-like receptor 4 ligands, and are largely resistant to intestinal proteases, eliciting a mild inflammatory response within the intestine after oral ingestion. Importantly, nutritional ATIs exacerbated inflammatory bowel disease and features of fatty liver disease and the metabolic syndrome in mice. For Alzheimer’s disease (AD), both inflammation and altered insulin resistance are major contributing factors, impacting onset as well as progression of this devastating brain disorder in patients. In this study, we evaluated the impact of dietary ATIs on a well-known rodent model of AD (5xFAD). We assessed metabolic, behavioral, inflammatory, and microbial changes in mice consuming different dietary regimes with and without ATIs, consumed ad libitum for eight weeks. We demonstrate that ATIs, with or without a gluten matrix, had an impact on the metabolism and gut microbiota of 5xFAD mice, aggravating pathological hallmarks of AD. If these findings can be translated to patients, an ATI-depleted diet might offer an alternative therapeutic option for AD and warrants clinical intervention studies.

Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

We previously demonstrated that a common dietary protein component, wheat amylase trypsin inhibitors (ATI), stimulate intestinal macrophages and dendritic cells via toll like receptor 4. Activation of these intestinal myeloid cells elicits an inflammatory signal that is propagated to mesenteric lymph nodes, and that can facilitate extraintestinal inflammation. Mice were fed a well-defined high fat diet, with (HFD/ATI) or without (HFD) nutritionally irrelevant amounts of ATI. Mice on HFD/ATI developed only mild signs of intestinal inflammation and myeloid cell activation but displayed significantly higher serum triglycerides and transaminases compared to mice on HFD alone. Moreover, they showed increased visceral and liver fat, and a higher insulin resistance. ATI feeding promoted liver and adipose tissue inflammation, with M1-type macrophage polarization and infiltration, and enhanced liver fibrogenesis. Gluten, the major protein component of wheat, did not induce these pathologies. Therefore, wheat ATI ingestion in minute quantities comparable to human daily wheat consumption exacerbated features of the metabolic syndrome and non-alcoholic steatohepatitis, despite its irrelevant caloric value.