mannosylated liposomes
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2021 ◽  
Vol 11 (5-S) ◽  
pp. 113-118
Author(s):  
Anuradha Soni ◽  
Vivek Jain ◽  
Sunil Kumar Jain ◽  
Pushpendra Kumar Khangar

Visceral leishmaniasis (VL) is a chronic debilitating disease prevalent in tropical and subtropical regions, caused by protozoan parasites of the genus Leishmania. Annually, it is approximated the occurrence of 0.2 to 0.4 million novel cases of the disease worldwide. The cast film method was used to prepared cationic and mannosylated liposomes. The surface of the Amphotericin B (Amp B)-bearing cationic multilamellar liposomes was covalently coupled with p-aminophenyl-α-D-mannoside using glutaraldehyde as a coupling agent, which was proved by agglutination of the vesicles with concanavalin A. The prepared liposomes were characterized for shape, size, % drug entrapment, vesicle count, zeta potential and in vitro drug release. Vesicle sizes of cationic and mannosylated liposomes were establish to be 2.71±0.12and 1.62±0.08μm, respectively. Zeta potential of cationic liposomes was higher (28.38 ± 0.3 mV), as compared to mannosylated liposomes (15.7 ± 0.8 mV). % drug release from cationic and mannose-coupled liposomes was established to be 45.7% and 41.9%, respectively, after 24 hrs. In the present work, cationic and mannosylated liposomes of Amp B were prepared, optimized and characterized for effectual organization of VL. Keywords: Mannosylated liposomes, Amphotericin B, Leishmaniasis, % drug release.


2021 ◽  
Vol 17 (12) ◽  
pp. 2455-2465
Author(s):  
Jing Chen ◽  
Yuan Lin ◽  
Min Wu ◽  
Chuangnan Li ◽  
Yimin Zhang ◽  
...  

This research was performed to optimize the enzymatic synthesis of mannosylated ligand with which to prepare mannosy-lated liposomes and investigate their bioactivity. Based on single-factor studies, lipase dose, substrate molar ratio (diester lauric diacid-cholesterol to mannose) and temperature were identified as significant parameters, and optimal reaction conditions were determined through response surface methodology (RSM) with central composite design. The optimum operating parameters, 61.23 mg of lipase, a substrate molar ratio of 5.36, and 56.64 °C temperature offered a predicted yield (71.11%) which was consistent with the actual yield (69.08%). Drug-free mannosylated liposomes were prepared film-dispersion. The characterizations of these liposomes showed that mannosylated liposomes were well-dispersible spherical particles with an average particle size of 142.3 nm, the polydispersity index of 0.16, and a zeta potential of −19.8 mV. Pyrogen examination, hemolytic studies and cytotoxicity assays revealed no substantial safety concern for drug-free mannosylated liposomes. Cellular uptake efficiency of mannosylated liposomes by HepG2 cells was significantly higher than that of unmodified liposomes, demonstrating that mannosylated ligands have a positive effect on liver targeting. Overall, mannosylated liposomes could be active drug delivery system for combatting the therapy of hepatic diseases.


Vaccine ◽  
2020 ◽  
Vol 38 (6) ◽  
pp. 1494-1504
Author(s):  
Aloysious Ssemaganda ◽  
Ashwini Kumar Giddam ◽  
Leanne M. Low ◽  
Xue Q. Liu ◽  
Mei-Fong Ho ◽  
...  

2019 ◽  
Vol Volume 14 ◽  
pp. 3203-3220 ◽  
Author(s):  
Jun Ye ◽  
Yanfang Yang ◽  
Wujun Dong ◽  
Yue Gao ◽  
Yingying Meng ◽  
...  

2017 ◽  
Vol 34 (6) ◽  
pp. 513-521 ◽  
Author(s):  
Menghua Xiong ◽  
Qian Lei ◽  
Xinyu You ◽  
Tiantao Gao ◽  
Xuejiao Song ◽  
...  

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