ANTIHYPERLIPIDEMIC EFFECT OF ETHANOLIC SEED EXTRACT OF CANAVALIA ENSIFORMIS (L) IN HIGH FAT DIET-STREPTOZOTOCIN INDUCED RATS

Objective: It was investigated to enlighten the phytochemical screening and antihyperlipidemic activity of seeds of Canavalia ensiformis (L.) in High fat diet Streptozotocin-induced diabetic rats.Methods: The ethanolic seed extract of Canavalia ensiformis (EECE) at a dose of 400 mg/kg and 200 mg/kg was administered to diabetic rats. Simvastatin (4 mg/kg) is used as a standard drug.Results: The statistics were assessed by using one way ANOVA followed by Dunnet’s multiple comparison tests. To unfold the mechanism we studied all the biochemical parameters like Total cholesterol (TC), Triglycerides (TG), high-density lipoproteins (HDL), low-density lipoproteins (LDL) and histopathological examination of Liver tissue section. The ethanolic extracts of seeds of Canavalia ensiformis showed significant reduce of the level of cholesterol, triglycerides, LDL, VLDL and a significant increase in the serum level of HDL at 400 mg/kg rather than 200 mg/kg.Conclusion: Further the characterization and isolation of the constituents could be done to know the exact mechanism of hyperlipidemic activity. Statistical analysis of this screening method conforms that the proposed method is appropriate and it can be useful, gives the basic idea to the researcher who is working in an area like Antihyperlipidemic activity.

Inhibitory Effect of TCCE on CCl4-induced Overexpression of IL-6 in Acute Liver Injury

Terminalia catappa L. leaves have been shown to protect against acute liver injury produced by some hepatotoxicants, but the active components and mechanisms are not clear. This study was designed to characterize the protective effects of the chloroform fraction of the ethanol extract of T. catappa leaves (TCCE) against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice, and analyze the changes in expression level of interleukin-6 (IL-6) in the process. It was found that TCCE pretreatment (10 or 30 mg/kg, ig) protected mice from CCl4 toxicity, as evidenced by the reversed alterations in serum alanine aminotransferase (sALT) and serum aspartate aminotransferase (sAST) activities. Additionally liver tissues were subjected to RT-PCR, Western blot and immunohistochemistry to analyze changes in IL-6 expression. It was found that TCCE markedly suppressed the CCl4-induced over-transcription of IL-6 gene. Consistent with the result, the expression of IL-6 protein was also blocked by TCCE in CCl4-stimulated mice, especially in the area around central vein on liver tissue section. In conclusion, TCCE is effective in protecting mice from the hepatotoxicity produced by CCl4, and the mechanisms underlying its protective effects may be related to the inhibition on the overexpression of IL-6 mainly around terminal hepatic vein.