CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation

Summary Mutation in CFAP43 leads to severe asthenozoospermia and multiple morphological abnormalities of the sperm flagellum (MMAF) in both human and mouse. Previous studies have shown that disruption of intra-manchette transport (IMT) caused failure of flagellum assembly and sperm head shaping. In a previous study, therefore, we postulated that disruption of IMT may contribute to the failure of sperm flagellum formation and result in MMAF, however the mechanisms underlying these defects are still poorly understood. Cfap43-deficient mice were studied here to reveal the cellular mechanisms of abnormal sperm head morphology and MMAF. Depletion of Cfap43 led to abnormal spermiogenesis and caused MMAF, sperm head abnormality and oligozoospermia. Furthermore, both abnormal manchette and disorganized ectoplasmic specialization (ES) could be observed at the elongated spermatids in Cfap43-deficient mice. Therefore, our findings demonstrated that, in mice, CFAP43-mediated IMT is essential for sperm head shaping and sperm flagellum formation.

Evaluation of sperm head abnormality traits and anti-bacterial properties of Achillea millefolium methanolic extract on methotrexate induced albino male mice.

Plants have been used by people from prehistoric times to get rid of suffering and curing ailments. Plants are of the important sources of medicine and a large numbers of drugs in use are derived from plants. Yarrow, a member of the aster family, is closely related to chrysanthemums and chamomile. Yarrow (Achillea millefolium) was named after Achilles, the Greek mythical figure who used it to stop the bleeding wounds of his soldiers. Decoctions have been used to treat inflammations, such as hemorrhoids, and headaches. Yarrow contains flavonoids (plant-based chemicals) that increase saliva and stomach acid, helping to improve digestion. Phenolic acids such as caffeic acid and salicylic acid are components of the bioactive compounds present in Achillea millefolium that have anti-inflammatory and anti-bacterial properties. This study was designed to assess the fertility and anti-bacterial activity of Yarrow methanolic extract through in vivo (albino male mice) and in vitro (antibacterial activity) studies. Results indicated that higher doses of plant increased sperm head abnormality and this lead to decrease fertility in addition to that antibacterial activity was dose dependant manner. Conclusions: Yarrow contains flavonoids and other chemical constituents which have these effects.

Effects of Ranitidine (Zantac) Drug on the Hormonal Level, Sperm Head Abnormality and Histo-Architecture of the Testis of Albino Male Mice

The purposes of this study are to determine the effect of Zantac (Ranitidine) drug on sexual hormones (Testosterone and Prolactin), sperm head abnormality, and histopathological activity on albino male mice testes. Two doses of the drug were used: 1000 and 2000 mg/kg, in addition to a negative control group. Each group included four mice and the drug administrated orally as (0.1 ml) per day for 14 days, and then the mice were sacrificed on the day 15 for laboratory assessment. The result showed that the drug cause increase the percentage of sperm head abnormality which reaches to (48.3%) and (22.8%) for 2000 mg/kg and 1000 mg/kg respectively in comparison to control group (11.1%). The male sex hormones also affected by the drug and the level of testosterone hormone decrease to (1.07 ng/ml) in 2000 mg/kg and (3.42 ng/ml) in 1000 mg/kg, while the level of hormone in control group is (14.07 ng/ml). The prolactin hormone show increase in the level at dose 2000mg/kg it's (115 ng/ml) and in dose 1000 mg/kg the level its (43 ng/ml) these value very high compared with control group (36 ng/ml) due to the effect of the drug on the hormone. The histopathological examination shows damage in the seminal duct and changes in the wall of the seminiferous tube also the spermatogenesis well be affected by the drug in which is stop and some of seminal duct show no appearance of spermatogenesis and also cause depletion to the wall of the seminal duct.

Studying some Cytotoxic Parameters of Aspartame (Diet sweet) on Mature Albino Male Mice

The present study was planned to evaluate some cytotoxic effect of aspartame intake trough determining the apoptosis cells from peripheral blood and sperm head abnormality from cauda epididymis. After one week of administration of various doses of asparatame (3.5,35,350 mg/kg) to mature albino male mice and distilled water treated group was served as control. The results were statistically analyzed to compare the data of individual dose with that of the control, apoptotic and necrotic cells as well as sperm head are determined. The results showed that induce apoptosis cells in all concentrations (3.5,35,350 mg/kg) and necrotic cells in doses (35, 350 mg/kg) while late apoptosis in doses (35, 3.5 mg/kg) on the other hand, asparatame is increased in sperm head abnormality in (350, 35 mg/kg) dose. According to these results, it was possible to conclude that aspartame have a cytotoxic risk. Therefore, it is necessary to be careful when using such materials in food and beverages as a sweetener.

Gambaran Morfologi Spermatozoa pada Perokok Sedang di Lingkungan PE Group yang Datang ke Bagian Biologi Fakultas Kedokteran Universitas Andalas

AbstrakTingginya angka pria perokok di seluruh dunia dan fakta bahwa asap rokok mengandung lebih dari 4000 bahan berbahaya yang dapat mengganggu sistem reproduksi. Gangguan yang terjadi dapat berupa penurunan kualitas spermatozoa yang dapat dilihat melalui analisis sperma. Tujuan penelitian ini adalah untuk melihat gambaran morfologi spermatozoa pada Perokok sedang di Lingkungan PE Group. Ini merupakan penelitian deskriptif dari September 2012 hingga 2013, menggunakan metode total sampling sebanyak 33 pria perokok yang mendatangi Laboratorium Biologi Fakultas Kedokteran Universitas Andalas Padang. Parameter kelainan morfologi spermatozoa (teratozoospermia) diukur menurut kriteria WHO yaitu apabila jumlah sperma dengan bentuk normal yang dicacah ≥ 30%. Semua sampel menunjukkan hasil yang normal. Kesimpulan hasil penelitian ini adalah perokok sedang yang diteliti memiliki gambaran morfologi spermatozoa yang normal, dengan abnormalitas kepala sebagai abnormalitas terbanyak dan bentuk kelainan kepala besar sebagai bentuk kelainan yang paling banyak ditemukan.Kata kunci: rokok sigaret, analisis sperma, morfologi sperma, teratozoospermia AbstractThe large number of men worldwide smoke and the fact that cigarette smoke contains detriment substances that can affect the health holistically and especially fertility it self is the main reason. It contains more than four thousands of detrimental substances for instance oxidants, carcinogenic and mutagenic agents. The objective of this study was to evaluate sperm parameters especially it’s morphology among medium smokers in PE Group, ltd. In this descriptive study, started from September 2012 until 2013. A total of thirty three males who were classified as medium smokers were enrolled to Biology Laboratory Medical Faculty of Andalas University Padang. Teratozoospermia parameters were measured according to the World Health Organization criteria and Biology Infertility Division’s in which the normal Sperm morphology counts ≥30%. All of samples showed normal results, with the sperm-head abnormality as the major abnormality. The conclusions is all participants showed normal spermatozoa morphology counts and the head abnormality is the major detriments.Keywords: cigarette smoking, sperm analyzing, sperm morphology, teratozoospermia

Curcumin Protects Metronidazole and X-ray Induced Cytotoxicity and Oxidative Stress in Male Germ Cells in Mice

Metronidazole (MTZ) is a common antiparasitic and antibacterial drug. The drug and X-ray induced effects in mouse sperm count, sperm head abnormality and some oxidative stress parameters have been studied. Simultaneously the protective role of curcumin has been evaluated. MTZ, 40 mg/kg bw and 13.4 mg/kg bw × 3 days exposure induced abnormal sperm head and reduced total sperm count in Swiss albino mice both after 24 h and 35 days of exposure. However, MTZ treatment in mice receiving X-ray irradiation (0.5 Gy) did not changed the incidence of abnormal sperm population or decrease in the total sperm count. Hepatic glutathione (GSH), superoxide dismutase (SOD) declined significantly during the treatment phase with significant rise in lipid peroxidation of the tissue. The cellular changes were estimated by using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS). Elevated TBARS is indicative of oxidative stress in treated mice. Furthermore, curcumin pre-treatment apparently reduced the frequency of sperm head abnormality and TBARS induced by MTZ alone or in combination with X-ray and increased the levels of hepatic GSH and SOD. The additive effects of MTZ and X-ray could not be observed with regards to sperm head abnormality and total sperm count in the tested dose range although the cellular antioxidants were found to be significantly lowered and lipid peroxidantion increased. The possible role of MTZ and X-ray inducing sperm abnormality, decreased sperm count and alteration in GSH, SOD and TBARS and defensive role of curcumin have been evaluated.

Role of vitamin E-acetate on cisplatin induced genotoxicity: An in vivo analysis

Vitamin E has generated immense interest because of its potential of being an antioxidant, a neuroprotector, and a protector against atherosclerosis, carcinogenesis and cardiovascular disease. However, the prooxidant chemistry of vitamin E cannot be ignored since it is related to the generation of peroxyl radicals. In the present study, 125, 250 and 500 mg/kg of vitamin E-acetate (VE) administered intraperitoneally (i.p.) to Balb/C mice significantly induced 6%, 8% and 11.33% (control value=2.33%) of chromosome aberrations (CA) and 0.88%, 1.39% and 1.81% (control value=0.61%) of micronucleus (MN), following 24 hour of treatment in the bone marrow cells. In the germ cells, VE did not induce any sperm head abnormality (SHA) after 35 days of exposure. Most importantly, it has been observed that pre-treatment with VE significantly reduces CA, MN, and SHA induction by chemotherapeutic drug cisplatin (CIS). Our findings suggest that lone treatment with VE induce genotoxicity in somatic cells after 24 and 48 hours of exposure but not in germ cells after 35 days of exposure, whereas pre-treatment with VE reduces CIS induced genotoxicity as well as cytotoxicity. There exists a thin line of difference on the behavioral transition of VE when acting alone and when acting with a drug.