New fluorescent imaging technics in gastrology

Introduction. There is a need to develop a new imaging technique in medicine. Gastroenterology is the branch of medicine focused on the digestive system and its disorders therefore for this branch is needed to detect all problems affecting the gastrointestinal tract. Aim. The aim of this article is to complete discuss the possibility of the new fluorescent imaging technics in gastrology to use innovative screening to identify individuals at an early stage. Material and methods. We discuss here imaging techniques such as include x-rays, computed tomography, scans, and magnetic resonance imaging in gastrology. Spectroscopy is the study of the formation and interpretation of spectra resulting from the interaction of all types of radiation on matter understood as a community of atoms and molecules. Various spectroscopic techniques are obtained by combining different types of radiation with different ways of its interaction with the test sample. They provide the opportunity to obtain detailed information about the tested substance – from its atomic composition, through its chemical structure, to its surface structure. Analysis of the literatue. The tissue fluorescence spectrum can be obtained by: (1) autofluorescence, or natural or primary fluorescence, i.e. by direct irradiation of the tissue with laser radiation (laser-induced fluorescence – LIF) and (2) photodynamic diagnosis (PDD), where spectrum analysis is preceded by systemic or local administration of the photosensitizer. Conclusion. The use of fluorescence imaging in colon cancer patient has potential to improve quality of treatment and diagnosis.

The degree of ripening of the grapevine in the varieties of selection of National Scientific Centre ‟Institute of viticulture and wine-making after V.YE. Tairovˮ in the conditions of the northern part of the Forest-steppe of Ukraine

Strong growth of shoots and ripening of grape fruits do not give grounds for claiming that the variety is in accordance with the climatic conditions of a certain area. Only a well-ripe vine can withstand severe winter conditions. The purpose of the study is to determine the degree of maturation of the vine in grape varieties of Odessa breeding grown in the northern part of the Forest-steppe, and to identify among them the most prepared for wintering conditions in the area. The degree of maturation of the vine was determined during 2018–2019 in the autumn before the cover of grape plants by the method of primary fluorescence using a microscope MBS-2 with fluorescent illuminator OI-18 and by the method of N. Matuzok. Studies have shown that the degree of ripening of the grapevine depends on the weather conditions during the growing season and varietal characteristics of the plants. Weather conditions during the years of study contributed to the good maturation of the shoots of grapes of all varieties except Kometa and Zahadka. The first had a characteristic brown color of the mature bark along the entire length of the shoot. When bending the vine in its upper part of the characteristic cracking of the bark was not in the varieties Kometa, Zahadka, Yarylo and Muskat odeskyi. The highest degree of ripening of shoot tissues was noted in the lower zone of the vine in the Aromatnyi, Kardyshakh, Kishmish tayirovskij. In the middle zone of shoots, the highest degree of maturation of the felema was found in the variety Kardyshakh, and in the upper part of the shoot, in the variety Aromatnyi. The highest conditional coefficient of maturation (Kv) of the tissues of the vine in the fifth internode is defined in the varieties Aromatnyi and Kyshmysh tairovskyi (0.90), the mean in the variety Kardyshakh (0.88), and the lowest in the variety Kometa (0.56). Based on the data obtained from the results of complex studies, it was found that the varieties Aromatnyi, Kyshmysh tairovskyi and Kardyshakh are the most prepared for wintering in the northern part of the Forest-steppe.

Aminomethylpyrene-based imino-phenols as primary fluorescence switch-on sensors for Al3+ in solution and in Vero cells and their complexes as secondary recognition ensembles toward pyrophosphate

Three aminomethylpyrene-based salicyl-imines, viz.L1, L2 and L3 were designed and synthesized for Al3+ ion sensing.

Imino–phenolic–azodye appended rhodamine as a primary fluorescence “off–on” chemosensor for tin (Sn4+) in solution and in RAW cells and the recognition of sulphide by [AR–Sn]

A new azo-rhodamine based, AR was developed for fluorescent ‘turn-on’ sensing of Sn4+ ion.

Unification of “Standard Background” Technique using Scattered Radiation in X-Ray Fluorescence Analysis (XRF)

Some x-ray fluorescence - concentration relationships in the framework of XRF were researched. Fundamental calculation approaches for primary fluorescence and incoherent scattering were realized for evaluation of matrix influence. A new binary approach was produced for the cases considered, and its unification was related to the empirical and regression types of the “standard background” technique, widely used in the analytical practice of XRF. It is confirmed that application of the calculations by fundamental parameters (FP) in combination with the empirical approach allows the reduction of the set of standards (to as few as one) in the analysis procedure with wide variations in matrices and concentrations, without loss of accuracy.

The Steady-State and Decay Characteristics of Primary Fluorescence from Live Bacteria

The intrinsic steady-state fluorescence and fluorescence decay of Staphylococcus epidermidis, Pseudomonas fluorescens, Enterobacter cloacae, Escherichia coli, and Bacillus subtilis have been observed. Excitation spectra were obtained while emission at 430, 455, 487 and 514 nm was being monitored. Emission spectra were obtained with the use of excitation wavelengths of 340, 365, 405, 430 and 460 nm. Fluorescence lifetimes were measured at 430, 487, and 514 nm while selective excitation was caused at 340, 405, and 430 nm. The complex nature of the excitation and emission spectra reflects the presence of a number of different fluorophores. Attempts have been made to describe portions of the bacterial fluorescence in terms of the measured fluorescence properties including lifetimes of molecular components known for their widespread occurrence in bacteria and their relatively high quantum yields. Candidate fluorophores which have been considered include the pteridines, the structurally related flavins, and the pyridine coenzymes. The observation that characteristic sets of lifetimes have been obtained for each organism suggests that measurements of fluorescence lifetimes may be helpful in the rapid characterization of bacteria. Results are especially definitive in cases such as Pseudomonas fluorescens, where one marker fluorophore, a pteridine, is produced in large amounts.