Resistance of nitrifiers inhabiting activated sludge to ciliate grazing

We monitored the succession of nitrifiers in a ne.wly opened wastewater treatment plant for five weeks. After the first distinct decrease in total nitrogen, we began monitoring the appearance, size and number of nitrifying bacteria colonies using the fluorescence in situ hybridization (FISH) method. Ammonia oxidizing bacteria (AOB) colonies were visualized under green excitation as red, and nitrite oxidizing bacteria (NOB) colonies were visualized under blue excitation as green. The changes in protozoan community were monitored simultaneously. Ciliates were divided into four functional groups: predatory, bacterivorous free-swimming, bacterivorous crawling, and sessile. The results showed that at the time of the first distinct total nitrogen decrease, the mean length of both AOB and NOB were relatively low, but the colonies, especially those of nitrite oxidizers, were abundant. In time, the distribution of ammonia oxidizer colonies shifted towards larger sizes, but their quantity decreased. In the case of nitrite oxidizers, a similar trend was noticeable but less pronounced. These changes corresponded with an increasing number of crawling bacterivorous ciliates dominated by the “scavenger” genus Aspidisca. The increasing size of nitrifier colonies may have been due to the growing grazing pressure from crawling bacterivorous ciliates. The strong grazing pressure did not negatively affect N-NH4+ removal effectiveness.

IDENTIFICATION OF TREATED-COLOR FRESHWATER CULTURED PEARLS

Demand for colored pearls has grown during the last thirty years. Colored pearls are rarer than white ones. Thus treated-color pearls have entered the marketplace and their identification became a challenge for the gemologists. With only the help of visual observation, EDXRF and X-radiography, methods that are used today for pearls identification, it is not always easy to identify them. Previous studies, have established that Raman scattering is useful to detect pigments in cultured freshwater pearls. The present study is based on the measurement of the Raman spectroscopy of 35 natural colored freshwater pearls and 15 treated-color freshwater pearls, covering a wide range of typical colors for this material, with green excitation. All natural- color pearls show the two major Raman resonance features of polacetyenic pigments assigned to C=C stretching-at about 1530(±25) cm'1- and C-C stretching - at about 1130(±10) cm' -, regardless of their specific hue. In this paper it is proposed that the absence of these Raman features prove the artificial origin of pigments in a colored freshwater cultured pearl.

Visualization of Hydatid Elements: Comparison of Several Techniques

Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus [“Taenia echinococcus”]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-rhodamine and Gram procedures failed to stain hooklets. Ziehl-Neelsen stain yielded indifferent results when organisms were viewed under transmitted light but resulted in a surprisingly intense red fluorescence when organisms were viewed under green light (excitation, 546 nm; long pass, 590 nm). Wheatley trichrome stain gave better and more uniform results than fuchsin. Ryan trichrome blue stain was the best under transmitted light; hooklets stained uniformly and intensely and were easily distinguishable from the background. Very satisfactory results were also obtained with a much simpler procedure (modified Baxby technique: no fixation, steaming hot 1% safranin for 2 min, and malachite green for 30 s). Therefore, Ryan and modified Baxby stains are recommended for the examination of E. granulosus under transmitted light. For fluorescence microscopy, Ziehl-Neelsen stain under green excitation light, or violet light with no staining, is also very useful. Epifluorescence microscopy is especially convenient for examining samples concentrated by filtration, as it renders the filter pores inconspicuous.

Microfluorometry of pectic materials in the dehiscence zone of almond (Prunus dulcis [Mill.] DA Webb) fruits.

We examined the middle lamella and the primary and secondary walls in almond pericarp dehiscence zone cells using a fluorescent cytochemical method which permitted specific, quantitative detection of pectic cell wall materials. Glycol methacrylate-embedded sections were stained with coriphosphine and pectin-specific fluorescent emissions at 630 nm were quantified using green excitation (546 nm). Examination of sectioned material extracted with purified pecto-lytic enzyme preparations was used to demonstrate the relative specificity of the staining reaction for pectic substances.