All Plant Breeding Technologies Are Equal, but Some Are More Equal Than Others: The Case of GM and Mutagenesis

A pervasive opposition to genetically modified (GM) foods has developed from the notion that they pose a risk to human and environmental health. Other techniques for the genetic modification of plants, such as sexual crossing and mutagenesis breeding, have mostly remained unchallenged. This research aims to investigate public perception of plant breeding technologies. Specifically, sexual crossing, mutagenesis, transgenics (GM) and gene editing. It was expected that attitudes and intentions would be most positive and the perception of risk lowest for plant genetic modification through sexual crosses. Scores on these variables were expected to be similar between mutagenesis, GM and gene editing. It was also expected that attitudes, intentions and risk perception would change (becoming more positive) once participants learned about foods developed through these technologies. Participants reported their attitudes, intentions and risk perception at two points in time. At Time 2, they were presented with pictures of food items developed through sexual crossing, GM and mutagenesis. The results showed that mutagenesis stood out as the most negatively perceived technology, whereas genetic development via sexual crosses was generally perceived as positive. The results highlight the importance of messaging, framing in consumer attitudes.

Movement Analysis to Associate Broiler Walking Ability with Gait Scoring

The genetic development of the commercial broiler has led to body misconfiguration and consequent walking disabilities, mainly at the slaughter age. The present study aimed to identify broiler locomotion ability using image analysis automatically. A total of 40 broilers that were 40 d old (male and female) were placed to walk on a specially built runway, and their locomotion was recorded. An image segmentation algorithm was developed, and the coordinates of the bird’s center of mass were extracted from the segmented images for each frame analyzed, and the unrest index (UI) was applied. We calculated the center of mass’s movement of the broiler walking lateral images capturing the bird’s displacement speed in the onward direction. Results indicated that broiler walking speed on the runway tends to decrease with the increase of the gait score. The locomotion did not differ between males or females. The proposed algorithm was efficient in predicting the broiler gait score based on their displacement speed.

AN OVERVIEW ON CHATURVIDHASHENHA (AYURVEDIC CLASSICAL FOUR TYPES OF FATS) IN SUTIKAPARICHARYA (PUERPERIUM)

Introduction : A group of human is nothing but society. Ayurveda not only explains individuals health but well commented on community health. In this context, Ayurveda described about Supraja (Healthy and Cultured progeny). Female has got an opportunity to nurture Supraja as she has capability of giving birth because of typical genetic development. For mother and child care diet, remedies, medicines, rules are mentioned in Ayurveda. (Aim). Hence topic was selected to review Chaturvidhasneha ( internal and external) in Sutikapricharya. Material and Method: Sutikaparicharya and Sheh Varga (group of unctuous food) were reviewed. Observation: A female, after expulsion of placenta at the end of labour is known as Sutika . According to classical references Sutika Kalavadhi is from 5th day to up to 45 th day. Involvement Trishosha, Saptadhatu, Upadhatu are observed during labour and Sutikaawastha. In Ayurvedic compendia, twenty four types of herbal unctuous substances are mentioned. Postnatal care period is indicated for consumption of these unctuous substances. Discussion: : In such condition, internal and external oleation helps to alleviate Tridosha, give strength to bodily tissues and rejuvenate deteriorated body organs involved during labour. Vegetable and animal are two sources of fats. Ghrita (cow ghee), Tila Taila (Sesame oil), Vasaa (animal muscle fat) and Majja (marrow) are described. Conclusion: Chaturvidhasneha plays an important role in Sutikaparichary., In this process, extensive depletion of Dhatu, Updhatu (Supportive tissue) and aggravation of Tridosha occurs. Chaturvidhasheha allivates Tridosha, recover depleted supportive tissue and helps in rejuvenation of body strength.

Comparison of the favorable gain values of genetic improvement among Indonesian grade cow breeds selected for agrotechnopark intensification

This research was aimed to compare the small and big truncation point proportions intended to evaluate gain values of genetic improvement for Indonesian Local cow breed groups selected for Agrotechnopark (integrated bio-cycle farming system) intensification. Animal live weights were collected from 674 Indonesian grade breed cows kept by local household farmers in North Sulawesi province. Data of cows were corrected by adjusting to six years old ages. All cows were divided into three breed groups with different genetic compositions of Bali breed cow generation (BG) of 207 cows, Ongole grade cow generation (OG) of 189 cows, and Local grade cow generation (LG) of 178 cows. The genetic improvements of BG, OG and LG were analyzed involving selection intensity (i), accuracy of selection (r), and standard deviation (SD) of breed group traits under selection. Results of this study showed that the critical components was genetic development of local grade breeds by choosing small proportion of 10% truncation point for Agrotechnopark intensification of selected elite cows groups among BG, OG and LG populations with the positive live weight gains of 58.6 kg, 23.15 kg, and 28.62 kg per generation, respectively compared with larger percentages of 20% and 30% proportions of truncation points.

Movement Analysis to Predict Broiler Walking Ability

The genetic development of commercial broiler led to body misconfiguration and consequent walking disabilities, mainly at the slaughter age. The present study aimed to identify broiler locomotion ability using image analysis automatically. A total of 40 broiler 40 d-old were placed to walk on a specially built runway, and their locomotion was recorded. An image segmentation algorithm was developed, and the coordinates of the bird's center of mass were extracted from the segmented images for each frame analyzed, and the Unrest Index (UI) was applied. We calculated the center of mass's movement of the broiler walking's lateral images, therefore, capturing the bird's displacement speed in the onward direction. Results indicated that broiler speed on the runway tends to decrease with the increase of the gait score. The locomotion did not differ between males or females. The proposed algorithm was efficient if predicting the broiler gait score based on their displacement speed.

Genetic Architecture Underlying the Metabolites of Chlorogenic Acid Biosynthesis in Populus tomentosa

Chlorogenic acid (CGA) plays a crucial role in defense response, immune regulation, and the response to abiotic stress in plants. However, the genetic regulatory network of CGA biosynthesis pathways in perennial plants remains unclear. Here, we investigated the genetic architecture for CGA biosynthesis using a metabolite-based genome-wide association study (mGWAS) and expression quantitative trait nucleotide (eQTN) mapping in a population of 300 accessions of Populus tomentosa. In total, we investigated 204 SNPs which were significantly associated with 11 metabolic traits, corresponding to 206 genes, and were mainly involved in metabolism and cell growth processes of P. tomentosa. We identified 874 eQTNs representing 1066 genes, in which the expression and interaction of causal genes affected phenotypic variation. Of these, 102 genes showed significant signatures of selection in three geographical populations, which provided insights into the adaptation of CGA biosynthesis to the local environment. Finally, we constructed a genetic network of six causal genes that coordinately regulate CGA biosynthesis, revealing the multiple regulatory patterns affecting CGA accumulation in P. tomentosa. Our study provides a multiomics strategy for understanding the genetic basis underlying the natural variation in the CGA biosynthetic metabolites of Populus, which will enhance the genetic development of abiotic-resistance varieties in forest trees.

EPEN-05. CLINICAL AND GENETIC EVOLUTION OF EPENDYMOMA EXPOSED FROM A MULTI-RECURRENCE GIRL CASE

Ependymomas are glial brain tumors accounting for approximately 2~3% of all primary tumors of the central nervous system (CNS), and 12% of all pediatric intracranial tumors. To better understand the evolution process of ependymomas, we studied the clinical, pathological and genetic development of a rare girl case with repeatedly recurrent ependymoma. This girl was diagnosed as ependymoma at age of 9 years old, and experienced 7 times tumor relapse and received 9 times surgeries but finally ceased at 19 years old with multiregional recurrences. The pathological characteristics, radiographic images and therapeutic strategies of the patient were all retrieved. Molecular markers confirmed the diagnosis of anaplastic ependymoma based on the updated WHO guideline for CNS tumors. Whole-genome sequencing (WGS) was performed to elucidate the landscape of mutation signatures and to identify potential driver mutations along the tumor progression. The seven tumor specimens showed a highly branched evolutionary pattern. There were six gene mutations found in 5 of the 7 specimens (PCDHA4, PCDHA8, SEC14L6, SETD2, RIOK2, and SLCO2A1) and three in 6 of 7 the samples (RYR1, SNX25, DSC2). Strikingly, there was one gene, ADGRL3, which was found to be consistently mutated in the entire disease progression process. Our findings therefore suggest that ADGRL3 might play roles in the disease progression of ependymoma patient.

EPCO-34. CLINICAL AND GENETIC EVOLUTION OF EPENDYMOMA

Ependymomas are glial brain tumors accounting for approximately 2~3% of all primary tumors in central nervous system (CNS), and 12% of all pediatric intracranial tumors. To better understand the evolution process of ependymomas, we studied the clinical, pathological and genetic development of a rare girl case with repeatedly recurrent ependymoma. This girl was diagnosed as anaplastic ependymoma at age of 9 years old, and experienced 7 times tumor relapse and received 9 times surgeries but finally ceased at19 years old with multiregional recurrences. The pathological characteristics, radiographic images and therapeutic strategies of the patient were all retrieved. Molecular markers confirmed the diagnosis based on the updated WHO guideline for CNS tumors. Whole-genome sequencing (WGS) was performed to elucidate the landscape of mutation signatures and to identify potential driver mutations along the tumor progression. The seven tumor specimens showed a highly branched evolutionary pattern. There were six gene mutations found in 5 of the 7 specimens (PCDHA4, PCDHA8, SEC14L6, SETD2, RIOK2, and SLCO2A1) and three in 6 of 7 the samples (RYR1, SNX25, DSC2). Strikingly, there was one gene, ADGRL3, which was found to be consistently mutated in the entire disease progression process. Our findings therefore suggest that ADGRL3 might play roles in the disease progression of ependymoma patient.

Nutrition Factor and Evolutionary Genetic Development of Intestinal Microflora: Importance for Maintaining Immunityand Health

The article considers the microbiota of modern people and its changes in the course of evolution under the influence of external and internal environment factors. The researchers explain the diversity loss and lack of beneficial microorganisms by the lack of carbohydrates available to the microflora (CAM) in the man diet, including dietary fiber fermented into short chain fatty acids (SCFA) - the main source of energy and intestinal microflora metabolism regulator. The authors demonstrated the intestinal microflora connection with other organs and systems of the human body, including the immune and nervous systems, and determined its significance in the occurrence and development of common diseases. Special attention is paid to the analysis of pro-, pre-, metabiotics and their metabolites, which have individual differences in the metabolic processes of a healthy and sick organism. The mechanisms and ways of implementing these functions depend on specific microorganism strains, that determines the prospects for creating and applying new biotechnological products with directed functional properties. A man noted antagonistic contradictions between own and produced strains. The article demonstrates that the microorganism genome is able to evolve in the struggle for survival, responding to changing nutritional conditions by delegating a huge number of genes to the human genome, indicating the need for nutrient-metabolic support of the microbiome. The issues of “aging” microbiota and the possibility of its support, as well as the transfer of beneficial bacteria from the donor to the recipient’s intestines, carrying out the transfer of positive physiological characteristics are interesting. The researchers discussed decoding process prospects of sequencing the genes that make up the human genome, the mechanisms of forming a personal microbiome for understanding the diseases pathogenesis and developing highly effective therapies. The most effective way to restore and preserve healthy microflora is a scientifically based diet with the additional inclusion of specialized products, the active principles of which are the producers of various bacteria.

KAJIAN APLIKASI TEKNIK INSEMINASI BUATAN DALAM PENINGKATAN GENETIK BOBOT BADAN TERNAK SAPI PERANAKAN ONGOLE DI SULAWESI UTARA

THE STUDY OF ARTIFICIAL INSEMINATION APPLICATION ON GENETIC IMPROVEMENT OF LIVE WEIGHT IN ONGOLE GRADE CATTLE AT NORTH SULAWESI PROVINCE. This research was conducted to evaluate the effective application of artificial insemination compared with natural breeding on genetic improvement by grading up system involving semen sourced from Ongole bull for the Indonesian-grade cattle at rural areas in Minahasa regency of North Sulawesi province, Indonesia. Data on animal live weight were collected from all cows (n= 404) kept by the traditional household farmers. Data of the cows were corrected by adjusting for the six years old of age. Breeding of beef genetic ancestors used the natural breeding (NB) and the artificial insemination (AI), divided into three cow groups with the first group of NB-mating without grading up (G0 cows), the second group of AI-mating with grading up using semen of Ongole bull for the first generation (G1 cows), and the third group of AI-mating with grading up using semen of Ongole bull for the second generation (G2 cows). The genetic improvements from breeding program of each G0, G1 and G2 were analyzed involving the selection intensity (i), the accuracy of selection (r), the genetic standard deviation of the trait under selection (SDg), and the generation interval (L) expressed in years. Results of this study showed that the critical components was the genetic development of local beef cattle by grading up using semen of the selected elite Ongole bull mated by AI method producing G2 in North Sulawesi province of Indonesia. This method increased genetically four hundred percents of live weight per year under 48% to 10% proportions of elite cows at the truncation points from the animal population compared with those of the natural mating of G0 in increasing genetic development for animal genetically live weight per year.Key Words: artificial insemination, genetic development, Indonesian local-grade cattle, Ongole breed bull