Morphological diagnosis of Setaria labiatopapillosa in domestic bovids from Marajó Island, Brazil

The genus Setaria is known worldwide for parasiting wild and domestic mammals, with 43 species registered, of which five occur in the American continent. The objective of this study was to characterize the occurrence, morphology and morphometry of S. labiatopapillosa in B. taurus and B. bubalis in Marajó Island, Brazil. Carcass inspections of 420 cattle were carried out in two slaughterhouses in Pará. The nematodes were found on the white and serous viscera of the small intestine, being cleaned and discussed for analysis by light microscopy and scanning electron microscopy. The morphological and morphometric characteristics are compatible with S. labiatopapillosa parasitizing both species, with a prevalence of 25% (B. bubalis) and 24% (B. taurus). Information obtained by scanning electron microscopy was added to the description of the species, in addition to expanding the distribution of this parasite in the national territory.

Ultrastructural and molecular characteristics of Setaria species based on sequence analysis of genomic and mitochondrial gene markers in cattle (Bos taurus) and buffaloes (Bubalus bubalis) from Iran

The aim of the present study was to investigate the ultrastructural characteristics and genetic diversity of Setaria parasites from cattle (n=696) and buffalo (n= 522) from Khuzestan province of Iran and to compare them with available data from other countries/regions by sequences analysis of the 12S Rdna and the mitochondrial cytochrome C oxidase subunit I (cox1) genes. Based on SEM (Scanning Electron Micrographs) and light microscopy, all the isolated worms were identified as Setaria labiatopapillosa. Our results showed that 12.3% of cattle were infected with Setaria spp., while no infection was found in buffaloes. The maximal prevalence was observed in cattle younger than one year old. The prevalence rate was not influenced by the season of the year or gender. Comparison of the obtained sequences from Setaria with sequences of Setaria spp. from GenBank confirmed that all samples belong to the species S. labiatopapillosa. The phylogenetic tree constructed using cox1 and 12S rDNA genes of several other filarial nematodes showed that the Khuzestan isolates share a common branch with S. labiatopapillosa from other regions. Intra-specific variation was observed in 12S rDNA but not in cox1. In conclusion, our results indicating that S. labiatopapillosa is the main species involved in the spread of setarial infection in south-west of Iran and the identified worms corresponded mostly to worms that reported previously throughout other continents.

Detection of parasitic helminths in cattle from Banda Aceh, Indonesia

Aim: The objective of this research was to identify the parasite species found in the gastrointestinal tract and pancreas of Aceh cattle slaughtered in a Banda Aceh slaughterhouse using lactophenol and semichon carmine staining. Materials and Methods: Each sample out of 50 samples of gastrointestinal tract and pancreas from Aceh cattle slaughtered in a Banda Aceh slaughterhouse was separated by organ. Each organ was examined for the presence of worm. Then, the parasitic worms found were subsequently collected and separated based on class and species, followed by staining using lactophenol and semichon carmine. The worms were then identified and their prevalence was determined. Results: The results showed that three species of parasites were successfully identified, all belonging to the nematode class, namely, Oesophagostomum radiatum, Oesophagostomum columbianum, and Setaria labiatopapillosa with the prevalence of 12%, 10%, and 6%, respectively. In addition, there was one species of parasite from the trematode class, namely, Eurytrema pancreaticum with prevalence of 0.4%. Conclusion: The nematode class worms, such as O. radiatum, O. columbianum, and S. labiatopapillosa, can be stained by lactophenol, while the trematode class worm such as E. pancreaticum can be stained by semichon's carmine.

The parasitic fauna of the European bison (Bison bonasus) (Linnaeus, 1758) and their impact on the conservation. Part 1 The summarising list of parasites noted

During the current century, 88 species of parasites have been recorded in Bison bonasus. These are 22 species of protozoa (Trypanosoma wrublewskii, T. theileri, Giardia sp., Sarcocystis cruzi, S. hirsuta, S. hominis, S. fusiformis, Neospora caninum, Toxoplasma gondii, Cryptosporidium sp., Eimeria cylindrica, E. subspherica, E. bovis, E. zuernii, E. canadensis, E. ellipsoidalis, E. alabamensis, E. bukidnonensis, E. auburnensis, E. pellita, E. brasiliensis, Babesia divergens), 4 trematodes species (Dicrocoelium dendriticum, Fasciola hepatica, Parafasciolopsis fasciolaemorpha, Paramphistomum cervi), 4 cestodes species (Taenia hydatigena larvae, Moniezia benedeni, M. expansa, Moniezia sp.), 43 nematodes species (Bunostomum trigonocephalum, B. phlebotomum, Chabertia ovina, Oesophagostomum radiatum, O. venulosum, Dictyocaulus filaria, D.viviparus, Nematodirella alcidis, Nematodirus europaeus, N. helvetianus, N. roscidus, N. filicollis, N. spathiger, Cooperia oncophora, C. pectinata, C. punctata, C. surnabada, Haemonchus contortus, Mazamastrongylus dagestanicus, Ostertagia lyrata, O. ostertagi, O. antipini, O. leptospicularis, O. kolchida, O. circumcincta, O. trifurcata, Spiculopteragia boehmi, S. mathevossiani, S. asymmetrica, Trichostrongylus axei, T. askivali, T. capricola, T. vitrinus, Ashworthius sidemi, Onchocerca lienalis, O. gutturosa, Setaria labiatopapillosa, Gongylonema pulchrum, Thelazia gulosa, T. skrjabini, T. rhodesi, Aonchotheca bilobata, Trichuris ovis), 7 mites (Demodex bisonianus, D. bovis, Demodex sp., Chorioptes bovis, Psoroptes equi, P. ovis, Sarcoptes scabiei), 4 Ixodidae ticks (Ixodes ricinus, I. persulcatus, I. hexagonus, Dermacentor reticulatus), 1 Mallophaga species (Bisonicola sedecimdecembrii), 1 Anoplura (Haematopinus eurysternus), and 2 Hippoboscidae flies (Lipoptena cervi, Melophagus ovinus). There are few monoxenous parasites, many typical for cattle and many newly acquired from Cervidae.

Epidémiologie des nématodes gastro-intestinaux des bovins dans la région centre de la Côte d'Ivoire

De février à décembre 1997, six bovins de race N'Dama, provenant de la zone centre de savane humide de la Côte d'Ivoire, ont été autopsiés chaque mois. Quatorze espèces ou genres de parasites ont été mis en évidence: 8 nématodes, 2 trématodes, 1 cestode et 3 protozoaires. Tous les animaux étaient infestés par au moins l'une des espèces suivantes de nématodes gastrointestinaux: Haemonchus sp. (95 p. 100), Cooperia punctata (50 p. 100), Oesophagostornum radiatum (35,5 p. 100), Trichostrongylus axei (30 p. 100), Cooperia pectinata (8,1 p. 100) et Trichuris sp. (1 6 p. 100). Setaria labiatopapillosa a été rencontrée chez 30,6 p. 100 des animaux, des microfilaires chez 24,1 p. 100, des paramphistomes chez 80,6 p. 100, et Moniezia sp. chez 15 p. 100. Des trypanosomes et Babesia spp. ont été trouvés chez respectivement 11,2 et 3,6 p. 100 des animaux. Les excrétions d'oeufs et les intensités parasitaires des nématodes gastro-intestinaux ont montré des fluctuations avec des pics en mai et en septembre-octobre, périodes correspondant aux saisons des pluies. Les animaux âgés de 1-3 ans avaient des intensités parasitaires plus élevées que ceux âgés de plus de 3 ans (P < 0,05). Aucune hypobiose larvaire n'a été détectée. En dépit de conditions climatiques humides favorables, les intensités parasitaires de ces bovins de race N'Dama, provenant de systèmes d'élevage de type extensif, étaient relativement faibles. Des traitements anthelminthiques ne seraient alors justifiés que chez les jeunes animaux.