Evaluation of bioactive compounds from Jasminum polyanthum and its medicinal properties

Jasmine plant species are widely grown in Asia and used for religious offering, is known to have wide range of bioactive compounds and its properties. Most of the jasmine species have been evaluated its bioactive properties and found positive results. This work consists of evaluating bioactive properties of jasmine species, Jasminum polyanthum and finding its potential medicinal uses. The plant leaves and flowers were powdered and added water to prepare extract. Both leaves and flower extract was evaluated for phytochemical compounds, anti oxidant, anti diabetic, anti inflammatory, anti microbial, anti cancer and DNA nicking assay. The leaf and flower extract contained most of the phytochemical compounds which leads to presence of various bioactive properties. Leaf possesses good DPPH activity, while flower possess high phenol content and FRAP activity. Leaf possesses good anti diabetic activity, while flower possess good anti inflammatory activity. Flower extract consists of higher antibacterial activity than leaf, while in antifungal it is vice versa. Keywords: Anti-diabetic, Anti-inflammatory, Antioxidant activity, MTT Assay, DNA Nicking study

Quaternary salts derived from 3-substituted quinuclidine as potential antioxidative and antimicrobial agents

Two series of novel ammonium salts containing the quinuclidine moiety were prepared in order to evaluate their antioxidative, antibacterial and antifungal potential. The synthesized homologues of 3-hydroxy (QOH) and 3-chloroquinuclidine (QCl) with the different N-benzyl substituents at the para-position (bromo, chloro or nitro group) were obtained in very good yields and characterized by IR and NMR spectroscopies and elemental analysis. All compounds were tested for antioxidative activity using the oxygen radical absorbance capacity (ORAC) assay and among tested samples, N-p-nitrobenzyl-3-hydroxyquinuclidinium bromide (QOH-4) exhibited the highest antioxidative potential (293.80 nmol (TE) mL-1), which was further investigated by the DNA nicking assay. The biological activity of selected compounds was evaluated by measuring the zone of inhibition and by determining the minimal inhibitory concentration (MIC) against three Gram-positive bacteria (B. cereus, E. faecalis and S. aureus), three Gram-negative bacteria (E. coli, P. aeruginosa and C. sakazakii) and three fungi species (C. albicans, A. niger and P. notatum). The bioactivity assay showed that some newly synthetized quaternary quinuclidinium compounds display a comparable or even better antibacterial and antifungal activity than the reference drugs such as gentamicin (GEN), cefotaxime (CTX) and amphotericin B (AMPHB). Among the tested compounds, N-p-chlorobenzyl-3-hydroxyquinuclidinium bromide (QOH-3) exhibited a considerable antibacterial efficiency against P. aeruginosa (MIC=0.39 µg mL-1) and QOH-4 displayed a potent antifungal activity against C. albicans (MIC=1.56 µg mL-1).

Phytochemical Analysis, Antioxidant (Dna Nicking Assay) and Antibacterial Activities of Morus Nigra L. Fruits Secondary Metabolites

The present study was carried out to identify the chemical composition of ethanol, flavonoid and anthocyanin extracts of Morus nigra fruits. The results revealed the presence of carbohydrates, glycosides, phenolic compounds and the absence of alkaloids, saponins, free amino acids, proteins and tannins. TLC and column chromatography techniques were used to separate and purify the anthocyanin pigment from anthocyanin extract which is then subjected to acid hydrolysis to obtain the anthocyanidin pigment (a). Many techniques were also employed to confirm pigment (a) chemical structure including (TLC, UV-Visible and IR-Spectroscopy). According to the forgoing analytical results, the proposed structure for the isolated pigment (a) is cyanidin pigment. DNA nicking assay has been used to evaluate the ability of the prepared extracts and pigment (a) to prevent DNA nicking caused by free radicals generated from Fenton's reagent. The highest DNA protective effect was achieved in anthocyanin extract followed by pigment (a) and their effects were identical to the positive control (cyanidin chloride). Antibacterial activities of all extracts and pigment (a) were performed against the growth of two standards and pathogenic G+ and G- bacteria. The results indicated that all plants extracts showed more potent activity against standard bacteria strains than pathogenic ones.