mechanisms of mutagenesis
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2021 ◽  
Vol 22 (11) ◽  
pp. 6144
Author(s):  
Christine J. Hawkins ◽  
Mark A. Miles

Many human cancers exhibit defects in key DNA damage response elements that can render tumors insensitive to the cell death-promoting properties of DNA-damaging therapies. Using agents that directly induce apoptosis by targeting apoptotic components, rather than relying on DNA damage to indirectly stimulate apoptosis of cancer cells, may overcome classical blocks exploited by cancer cells to evade apoptotic cell death. However, there is increasing evidence that cells surviving sublethal exposure to classical apoptotic signaling may recover with newly acquired genomic changes which may have oncogenic potential, and so could theoretically spur the development of subsequent cancers in cured patients. Encouragingly, cells surviving sublethal necroptotic signaling did not acquire mutations, suggesting that necroptosis-inducing anti-cancer drugs may be less likely to trigger therapy-related cancers. We are yet to develop effective direct inducers of other cell death pathways, and as such, data regarding the consequences of cells surviving sublethal stimulation of those pathways are still emerging. This review details the currently known mutagenic consequences of cells surviving different cell death signaling pathways, with implications for potential oncogenic transformation. Understanding the mechanisms of mutagenesis associated (or not) with various cell death pathways will guide us in the development of future therapeutics to minimize therapy-related side effects associated with DNA damage.


2020 ◽  
Vol 47 (1) ◽  
pp. 9-16
Author(s):  
B. Georgieva ◽  
S. Atemin ◽  
A. Todorova ◽  
T. Todorov ◽  
A. Miteva ◽  
...  

AbstractObjective: The aim of the study was to determine the molecular mechanisms of mutagenesis in Bulgarian patients with Angelman syndrome (AS). AS is a severe neurodevelopmental disorder caused by loss of expression in brain of the maternally inherited UBE3A gene as a result of various 15q11.2-q13 alterations.Material and Methods: In total 24 patients (11 boys, 13 girls) from 22 unrelated families with suspected clinical diagnosis AS were analysed. We used methylation specific PCR, multiplex ligation-dependent probe amplification, methylation sensitive MLPA, and direct sequencing of the UBE3A gene.Results: In 9 families (41%) pathogenic mutations were detected, which confirmed the clinical diagnosis on а molecular-genetic level. In 4 (44%) of these families we found 15q11-q13 region deletion with breakpoints BP1-BP3 or BP2-BP3. In 1 (11%) of the families we found imprinting defect: deletion of the AS-SRO regulatory region (part of the PWS-AS imprinting center). In 1 (11%) of the families we detected a rare finding – paternal uniparental disomy of chromosome 15. In 3 (33%) of the families diff erent point mutations in the UBE3A gene were detected: two novel missence mutations c.488T > C; p.Leu163Ser and c.1832A > T; p.Gln611Leu, and one known frameshift mutation c.2576_2579delAAGA; p.Lys859Argfs*4.Conclusion: The obtained results helped us to develop a systematic diagnostic algorithm in order to provide proper diagnosis for the patients with AS. Combining excellent knowledge of the molecular mechanisms of mutagenesis and proper molecular-genetic testing approaches is a cornerstone in the management of AS patients, ensuring AS families would receive both adequate genetic counseling and prophylaxis of the disease in the future.


2016 ◽  
Vol 768 ◽  
pp. 53-67 ◽  
Author(s):  
Binyan Liu ◽  
Qizhen Xue ◽  
Yong Tang ◽  
Jia Cao ◽  
F. Peter Guengerich ◽  
...  

2013 ◽  
Vol 752 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Barbara E. Wright ◽  
Karen H. Schmidt ◽  
Michael F. Minnick

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33563 ◽  
Author(s):  
Jun Nakamura ◽  
Husamettin Gul ◽  
Xu Tian ◽  
Scott J. Bultman ◽  
James A. Swenberg

2010 ◽  
Vol 39 (4) ◽  
pp. 1360-1371 ◽  
Author(s):  
D. Kumar ◽  
A. L. Abdulovic ◽  
J. Viberg ◽  
A. K. Nilsson ◽  
T. A. Kunkel ◽  
...  

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