Association of Val16Ala Polymorphism of Manganese Superoxide Dismutase (MnSOD) with Food Intake and Cardiometabolic Risk Factors in the Elderly in Primary Care in Porto Alegre.

Background: The aging process causes physiological changes on its own. The combination of an unhealthy lifestyle with the presence of genetic polymorphisms, such as the Val16Ala of the antioxidant enzyme manganese-dependent superoxide dismutase (MnSOD) may contribute to a greater occurrence of cardiometabolic risk factors. Objective: This study aimed to verify the association of Val16Ala-MnSOD polymorphism with food intake, caloric expenditure, and cardiometabolic risk factors in the elderly. Methods: A cross-sectional study with a sample size of 270 elderly individuals assisted in primary health care in the city of Porto Alegre, RS, Brazil. Val16Ala polymorphism, glucose, lipid profile, insulin, HOMA-IR, blood pressure, waist circumference, PCR-us, IL-6, food consumption, and caloric expenditure were evaluated. Results: The average age of the elderly was 68.6 ± 7.6 years. There were statistically significant differences regarding the consumption of two or more servings of fruits and vegetables daily between the elderly VV versus AV (P=0.017). There were also statistically significant differences regarding the consumption of two or more daily servings of legumes and eggs between the elderly AA versus VV (P=0.002). The median of insulin was higher in the elderly AA versus AV (P=0.025) and the median of HOMA-IR was higher in the elderly VV versus AV (P=0.029). AA elderly individuals had higher means of high-density lipoprotein (HDL-c) compared to AV (P=0.029). Conclusion: The results suggest that Val16Ala -MnSOD polymorphism is associated with the consumption of fruits, vegetables, legumes, and eggs, as well as with cardiometabolic risk factors in the elderly.

Meta-analysis of the relationship between MnSOD polymorphism and cancer in the Turkish and Cypriot population

Objectives: The association between manganese superoxide dismutase (MnSOD) p.Val16Ala polymorphism and cancer has been shown in various studies. The aim of this study is to investigate the relationship between MnSOD polymorphism (V/V, V/A, A/A) and cancer in the Turkish and Cypriot population through meta-analysis. Material and methods: The present study included meta-analysis of 14 publications covering 2413 cancer patients and 2907 healthy control groups from 2005 to 2016. Pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using the random effect model of DerSimonian and Laird for each study. Publication bias was checked with funnel plot by Begg’s and Egger’s test statistics. Results: Meta-analysis of MnSOD polymorphism was performed in the additive model (AV vs. VV; OR=1.133, 95% CI: 1.002–1.282), allele contrast (A vs. V; OR=1.016, 95% CI: 0.930–1.278), homozygote model (AA vs. VV; OR=0.983, 95% CI: 0.839–1.153), dominant model (AA+AV vs. VV; OR=1.090, 95% CI: 0.971–1.223) and recessive model (AA vs. AV+VV; OR=0.924, 95% CI: 0.803–1.064). The A/V genotype polymorphism was found be significant for cancer. Conclusion: The frequency of the A/V heterozygote genotype of the MnSOD polymorphisms is found to be higher in the Cypriot and Turkish populations than any other genotype.

The Relationship between ALA16VAL Single Gene Polymorphism and Renal Cell Carcinoma

Objectives. The aim of this study was to investigate the association of RCC and Ala16Val polymorphism in Turkish patients with RCC.Materials and Methods. A total of 41 patients with RCC who underwent radical or partial nephrectomy in our clinic and 50 healthy volunteers living in the same geographic area were included in this study. DNA samples from serum of RCC patients and controls were genotyped for MnSOD polymorphism analysis. Genotype ratios and allele frequencies were compared between two groups and odd ratios with 95% confidence intervals were calculated statistically. APvalue of <0.05 was considered statistically significant.Results. There was a significant difference in the MnSOD genotype distributions between the RCC patients and the controls in terms of Ala/Ala+Ala/Val and Val/Val genotypes (P=0.039). The Ala/Ala+Ala/Val genotypes were found significantly suspicious for RCC with an OR of 2.64 (95% CI = 1.06–6.69,P= 0.039). In addition, Ala allele was found significantly suspicious for RCC with an OR of 2.26 (95% CI = 1.24–4.12,P= 0.009).Conclusion. Our study indicated that MnSOD Ala16Val polymorphism may be one of the many genetic factors for renal cancer susceptibility in Turkish patients.