Detection of Antibiotic-Resistant Gene from Isolates of Diabetic Ulcer Patients

Antibiotic resistance can be caused by the presence of several genes encoding antibiotic resistance found in certain bacteria. S. aureus bacteria contained blaZ (97.4%), mecA (42.3%) and tetM genes (20.2%). Bacillus sp. contained blm (87.2%), tetL (44%) and tetB genes (2.9%). E. coli bacteria contained tem (80.9%)dan shv genes (14.28%). Clostridium spp. Bacteria contained cat gene (36%), as well as S. pyogens contained ermT (36.5%). Specific primers made in silico based on bioinformatics were then analyzed by PCR (Polymerase Chain Reaction) and electrophoresis. The purpose of this study was to detect the presence of blaZ, mecA, tetM, blM, tetL, tetB, tem, shv, cat, and ermT genes. The research began by searching for the nucleotide sequences for the ermT, cat, tetB, and blaZ genes through the Primer 3Plus program and analyzed using OligoAnalyzer 3.1. In vitro detection was carried out by extracting DNA and then amplification using PCR, and analyzed by agarose gel electrophoresis. The results of the analysis of the primer criteria for the ermT gene and the blaZ gene at the optimum Tm 55 C, cat gene and the tetB gene at the optimum Tm 60 C have met all the primer criteria. The results obtained are mecA gene (533 bp), tetM gene (366 bp), blm gene (1107 bp), tetL gene (267 bp), tetB gene (186 bp), tem gene (1073 bp), cat gene (164 bp), the ermT gene (202 bp) has been detected successfully, while blaZ and shv have not been detected successfully.

Pancreatic cancer in bloom syndrome

Bloom syndrome is a rare autosomal recessive disorder characterized by distinct physical features, such as short stature, genomic instability, and predisposition to numerous cancers. The BLM gene encodes for the RecQ helicase that plays an important role in genome editing, maintenance, and stability. Mutations in the BLM gene cause genomic instability that exposes the carriers to a variety of cancers, and in particular hematological and gastrointestinal cancers. Herein, we report the first case of pancreatic cancer in a 32-year-old patient with bloom syndrome.

Genomic instability and cancer: lessons from analysis of Bloom's syndrome

Bloom's syndrome (BS) is a rare autosomal recessive disorder characterized by genomic instability and cancer predisposition. The underlying genetic defect is mutation of the BLM gene, producing deficiency in the RecQ helicase BLM (Bloom's syndrome protein). The present article begins by introducing BLM and its binding partners before reviewing its known biochemical activities and its potential roles both as a pro-recombinase and as a suppressor of homologous recombination. Finally, the evidence for an emerging role in mitotic chromosome segregation is examined.