Reciprocal Chromosome Translocation Between the Left-End 220kb of Chromosome II and the Right-End 270kb of Chromosome X in Saccharomyces cerevisiae

Southern hybridization of chromosomes and the physical mapping of the genes used as several probes on the respective chromosomes II and X showed that the left-end ca. 220kb of chromosome II including ATP1 was exchanged the right-end ca. 270kb of chromosome X including ATP2 resulting the reciprocal chromosome translocation in the yeast strain YNN290, Saccharomyces cerevisiae. YTO290, the mutated strain by the reciprocal chromosome translocation as above described, was changed from red to white of the colony-color, and sizes of chromosome II lengthened from ca. 830kb to ca. 900kb and chromosome X shortened from ca. 760kb to ca. 690kb, respectively, in compared with the original strain YNN290. But, YTO290 strain was the same as the original strain YNN290 for other properties; the nutrient requiring of the genotype, the ploidy, the mitochondrial respiratory activity, the cell-size, and the growth-rate (doubling time), the number of chromosomes in a cell, It should be as a total number of nucleotides (bases) of genome.ATP1 or ATP2 and their neighboring base sequences respectively should be transferred from chromosome II left-end ca. 220kb to chromosome X right-end or chromosome X right-end ca. 270kb to chromosome II left-end accompanying with this reciprocal chromosome translocation. This mutated (the reciprocal chromosomes II and X translocation = exchanged those end-sequences as above described) strain, YTO290, seemed to lead to decrease the stability of the changed chromosomes II and X. The mutated strain, YTO290 might be observed to go back to the respective chromosomes II and X of the original strain, YNN290, in several months later even at 4°C.

Molecular Characterization of the t(8; 13)(p11;q12) Translocation Associated With an Atypical Myeloproliferative Disorder: Evidence for Three Discrete Loci Involved in Myeloid Leukemias on 8p11

A reciprocal chromosome translocation between 13q12 and 8p11 is the consistent cytogenetic abnormality seen in a nonspecific myeloproliferative disorder that is associated with T-cell leukemia/lymphoma and peripheral blood eosinophilia. Detailed molecular analyses of the translocation breakpoints associated with this rearrangement have not been reported to date. We have now generated somatic cell hybrids from a newly described patient with this specific structural rearrangement and analyzed the breakpoints on the derivative chromosomes. We have shown that the breakpoint on chromosome 13 lies within a 300- to 500-kb region defined by the KIAA177 gene and D13S1123 marker. In addition, we have identified a 1.2-Mb YAC, 959A4, that crosses the translocation breakpoint on the short arm of chromosome 8 in this patient. The location of this breakpoint in 8p11 is distinct from the t(8; 16) and t(8; 22) translocations associated with M4/M5 myeloid leukemias, and suggests that three distinct loci located within 8p11 are involved in the pathogenesis of myeloid neoplasias.