flash cooling
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2020 ◽  
Vol 117 ◽  
pp. 111288
Author(s):  
Adrian Borhan ◽  
Dumitru-Daniel Herea ◽  
Daniel Gherca ◽  
Cristina Stavila ◽  
Anca-Emanuela Minuti ◽  
...  

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0239706
Author(s):  
Jean-Marc Jeckelmann ◽  
Hüseyin Ilgü ◽  
Patrick D. Bosshart ◽  
Dimitrios Fotiadis

Crystals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 78
Author(s):  
Yoshinobu Hashizume ◽  
Koji Inaka ◽  
Naoki Furubayashi ◽  
Masayuki Kamo ◽  
Sachiko Takahashi ◽  
...  

In this paper, we present a summary on how to obtain protein crystals from which better diffraction images can be produced. In particular, we describe, in detail, quality evaluation of the protein sample, the crystallization conditions and methods, flash-cooling protection of the crystal, and crystallization under a microgravity environment. Our approach to protein crystallization relies on a theoretical understanding of the mechanisms of crystal growth. They are useful not only for space experiments, but also for crystallization in the laboratory.


Author(s):  
Yoshinobu Hashizume ◽  
Koji Inaka ◽  
Naoki Furubayashi ◽  
Masayuki Kamo ◽  
Sachiko Takahashi ◽  
...  

We summarize how to obtain protein crystals from which better diffraction images can be obtained. In particular, we describe in detail the quality evaluation of the protein sample, the crystallization methods and crystallization conditions, the flash-cooling protection of the crystal, and the crystallization under a microgravity environment.


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