Phase imaging of transition from classical to quantum plasmonic couplings between a metal nanoparticle and a metal surface

When a metal nanoparticle is brought near to a metal surface within electron tunneling distance (∼1 nm), classical electromagnetic coupling between the nanoparticle and the metal is expected to transition to quantum coupling. We show that this transition can be observed as a drastic phase change in the surface plasmon resonance (SPR) images of the gold nanoparticles. We study the transition by controlling the distance between the nanoparticles and electrode surface, modeling the impact of the transition on the SPR image in terms of a phase shift and demonstrating detection of microRNA based on the transition from classical to quantum coupling. The work shows that the quantum coupling can be directly visualized in SPR, and the extremely sensitive dependence of the transition on distance leads to a biosensing principle with SPR.

THE IMPACT OF NON-LOCAL ACTIVATION AND ASSOCIATED WATER PHASE PARA-STATE ON AN AQUATIC ORGANISMS DAPHNIA MAGNA LIFETIME

Introduction. The work is devoted to the study of associated water phase magnetic state influence, represented by amorphous ice with a modified ratio of isomers in the increasing of para-water proportion, and non-local water activation based on the technology of non-local quantum water conjugation with the production activating process, on the vital hydrobionts Daphnia magna signs. Material and methods. The studies were carried out using non-locally activated drinking water “Si” and a para-water concentrate prepared in hypomagnetic conditions at a residual magnetic flux density not more than 20ntl according to the manufacturer’s technology. The para-water concentrate was introduced into the hydrobionts medium in the ratio of 9 ml per 1 liter of water under study. To obtain offspring, individuals were used, derived on the settled tap water (1st generation) and individuals, derived on the water “Si” - 2nd generation. One-day individuals of Daphnia magna were placed in the amount of 20pcs/l of the studied water and the entire life cycle were kept in optimal conditions: climatostat P-2 (temperature - 20±1°C, illumination - 1200-2500lux, photoperiod - 12/12) hour. Feeding of Daphnia was carried out by Chlorella microalgae grown on the medium “Tamiya”-50% with a density D=0.65-0.72. Results. The life expectancy of Daphnia magna hydrobionts in the non-locally activated environment (water “Si”) increases by an average of 38 days in the first generation and 50 days in the second generation, and in an environment with a potentiating additive para-water associates for 58 days (first generation) and 41 days (second generation).The life expectancy of aquatic animals is related by negative correlation with the change in values of the oxidation-reductant potential (Eh) of the environment of hydrobionts development (Corr.=-0.993 (1st generation). Both non-local water activation and spin-modification of its associated phase by para-isomers demonstrate an increase in the hydrobionts productivity: - in non-locally activated water - 1.20 (1st generation) and 2.68 (2nd generation); - in non-locally activated water with the addition of para-water - 1.39 (1st generation) and 1.97 (2nd generation) times. Conclusion. From the results it follows that the determining factor in the hydrobionts medium is its quantum coupling with electron sources in the external environment. This determines the importance of water quantum coupling with natural ecosystems, affecting cellular metabolism. Spin para modification of the associated water phase leads to an additional increase in the hydrobionts life expectancy in the first generation. In the second generation, the influence of para-water is accompanied by some decrease in both the life hydrobionts expectancy and their productivity.