Molecular landscape of etioplast inner membranes in higher plants

Etioplasts are photosynthetically inactive plastids that accumulate when light levels are too low for chloroplast maturation. The etioplast inner membrane consists of a paracrystalline tubular lattice and peripheral, disk-shaped membranes, respectively known as the prolamellar body and prothylakoids. These distinct membrane regions are connected into one continuous compartment. To date, no structures of protein complexes in or at etioplast membranes have been reported. Here, we used electron cryo-tomography to explore the molecular membrane landscape of pea and maize etioplasts. Our tomographic reconstructions show that ATP synthase monomers are enriched in the prothylakoids, and plastid ribosomes in the tubular lattice. The entire tubular lattice is covered by regular helical arrays of a membrane-associated protein, which we identified as the 37-kDa enzyme, light-dependent protochlorophyllide oxidoreductase (LPOR). LPOR is the most abundant protein in the etioplast, where it is responsible for chlorophyll biosynthesis, photoprotection and defining the membrane geometry of the prolamellar body. Based on the 9-Å-resolution volume of the subtomogram average, we propose a structural model of membrane-associated LPOR.

Elastic behavior of composites reinforced by 3D printed tubular lattice braid textures

Purpose The purpose of this paper is to introduce an innovative transversal tubular braid texture and to study the elastic behavior of its 3 D printed structure comparatively to 3 D printed longitudinal tubular braid texture (maypole) to be used as reinforcement. Design/methodology/approach Regarding the lack of proper machines for the production of the proposed texture, the structure of samples was produced as a tubular lattice braid texture using a 3 D printer with the fused deposition modeling method subsequent to simulation by Rhinoceros software. The produced specimens were composited by polyurethane resin. The composite samples were evaluated by the split disk mechanical test to obtain their hoop stress. The structures of the reinforced composites were theoretically analyzed by ANSYS software. Findings The results of the mechanical test and theoretical analysis showed that the composites reinforced with transversal tubular lattice braid have higher strength compared to the composites reinforced with longitudinal ones. This assured that the composite reinforced by transversal tubular lattice braid is reliable to be used as high-performance tube for different applications. Originality/value Further work is carried out to produce the innovated complex structure continuously by a specially designed machine and fibrous materials to reinforce tubular composites in an industrial continual process to be applied for high-pressure fluids flows.