ANTIMICROBIAL STUDY OF PYROLIGNEOUS EXTRACT FROM RHIZOPHORA APICULATE AGAINST URINARY TRACT PATHOGENS

Various parts of the human body are host to certain microorganism species, such as fungi and bacteria which may cause urinary tract infections (UTIs). In this research, the pyroligneous extract as antimicrobial agent on urinary tract infection-related pathogenic microorganism were studied. The Time-kill kinetics assay was used to study an antimicrobial agent over time. A series of various concentration (0 – 24%) of the pyroligneous extract was tested. Each strain’s bioactivity was quantified after 60 minutes of incubation at 22.5 oC. From the result showed that pyroligneous extract reached 100% of reduction effects with different concentration (E.faecalis (19433) at 20%, E.coli (8739) at 4%, P.vulgaris (33420) at 4%), K.pneumoniae (13883) at 4%, C.albicans (10231) at 4%). For bioprocess kinetics analysis, the highest specific reduction rate and halve rate according concentration: E.faecalis at 24% (0.1426, 0.2058), E.coli at 4% (0.1219, 0.1759), P.vulgaris at 4% (0.1311, 0.1891), K.pneumoniae at 4% (0.1175, 0.1695), C.albicans at 4% (0.1175, 0.1695).These findings shows the importance of pyroligneous extract as antimicrobial agent, especially in urinary tract infection, as a natural antimicrobial agent in public health industries.

Monitoring the Kinetics of Bioprocess Variables – Theory and Applications

A new method for monitoring of bioprocess kinetics is developed where there exists a set of measured variables but the use of constant values of kinetic parameters is not acceptable. The method is based on new formalization of kinetics of biotechnological processes. It is generalized in one vector including unknown time-varying parameters only. For kinetics estimation, a general structure of software sensor is presented. A optimization procedure for tuning of the sensor’s parameters is proposed on the basis of stability and convergence analysis. The proposed method is applied for monitoring of bioprocesses for gluconic acid, alpha-amylase and ethanol production.