PULMONARY NEMATODOSIS OF THE BAIKAL SEAL (PUSA SIBIRICA)

Current data on the prevalence of pulmonary nematodes in Baikal seals and their effect on the host organism are not available. In October 2020, annual research expedition in order to study population, monitoring of territorial distribution and role of the Baikal seal in a closed ecosystem was undertaken. During this expedition 71 Baikal seals (Pusa sibirica) were caught and examined. The material was collected by complete helminthological dissection of individual organs, and samples were taken for histological examination. Found helminths were fixed in 70% ethanol, and cross-sections were prepared from organs and tissues. Pulmonary nematodes were recorded in 62 seals (87.3%). Parafilaroides krasheninnikovi were found in 62 seals (87.3%); large number of helminths were located on the surface and inside the lungs. Despite the high intensity and prevalence of the invasion, there were no serious pathological changes associated with P. krasheninnikovi. Otostrongylus circumlitus was found in 5 Baikal seals. Against the background of the O. circumlitus invasion in the studied Baikal seals, the development of limited granulomatous pneumonia foci was noted. However, the intensity (3.2 specimens) and prevalence (7%) of this invasion were low.

Granulomatous pneumonia due to pythiosis in captive South American coatis (Nasua nasua)

ABSTRACT: Pythiosis is an emerging infectious disease affecting captive and free-ranging wild animals. We report granulomatous pneumonia due to Pythium insidiosum in two South American coatis (Nasua nasua), who were found dead without any clinical records. Severe granulomatous pneumonia associated with pleural effusion was revealed in the necropsy. Microscopically, variably sized granulomas and pyogranulomas presented negative hyphae profiles at the periphery of their necrotic cores. Grocott methenamine silver stain highlighted these structures, and immunostain (anti- P. insidiosum) was strongly positive. Molecular analysis by polymerase chain reaction amplified P. insidiosum specific DNA. These findings characterized P. insidiosum as a cause of granulomatous pneumonia in coatis and proved that pythiosis needs to be considered in the differential diagnosis of respiratory diseases affecting this species in endemic areas.

37. Risk Factors for Granulomatous Pneumonia Incidence in Lampung Breeding Farm

Complex respiratory disease in the calf (enzootic calves pneumonia) is a complex respiratory disease (bovine respiratory disease complex/BRDC) caused by co-infection with viral, bacterial or fungal pathogens. Granulomatous pneumonia is one of the dominant lesions in BRDC that characterizes the infection of Mycoplasma bovis, a bacterium that is very strong against phagocytosis of immun cells. This study aims to determine the presence of risk factors associated with the incidence of granulomatous pneumonia. This study used secondary data from Laboratory of Pathology in Balai Veteriner Lampung, Sample collection in 2015-2017 period from one of the breeding farm in Lampung. Data is processed by calculating frequency, tables and logistic regression in Epi-info tools. The frequency of granulomatous pneumonia at the age of pre weaning and weaning was 63.06% and 36.94%, breed Wagyu and Brahman cross (76.12% and 23.88%), as well as males and females (61.01% and 38.99%). The results of measurements with Epi-info show that the age factor (Odds ratio = 2.16, 95% CI = 1.51-3.09) and breeds (Odds ratio = 1.52, 95% CI = 1.003-2.31) were associated with the incidence of granulomatous pneumonia. Based on the results of research that Wagyu calf and weaning age are more at risk of granulomatous pneumonia.

Adiaspiromycosis in a wild European rabbit, and a review of the literature

Adiaspiromycosis is a mycotic infection caused by thermally dimorphic fungi classified as Emmonsia parva and E. crescens (formerly Chrysosporium spp.) until recently, when new classifications were proposed. We document the pathologic findings in a severe case of adiaspiromycosis, with lymph node involvement, in a wild European rabbit ( Oryctolagus cuniculus). The rabbit exhibited granulomatous pneumonia with tracheobronchial lymph node enlargement. Histopathologically, the lung was expanded by myriad, densely cellular, heterophilic and granulomatous foci, surrounding bi- to trilaminar adiaspores. Adiaspore density was considered to be similar in all lung lobes. In the left caudal lung lobe, 80 adiaspores were counted in a 50-mm2 area using digital image analysis. The mean and median adiaspore diameters were 240 ± 52 μm and 255 μm, respectively. Tracheobronchial lymph nodes exhibited moderate numbers of similar adiaspores. PCR amplification of DNA extracted from microdissected adiaspores failed to identify Emmonsia spp.–specific DNA. These data suggest that adiaspiromycosis may result in severe granulomatous pneumonia in wild European rabbits. Although confirmation of the etiologic agent by PCR using DNA extracted from formalin-fixed tissue is not always successful, digital image analysis can be used to aid accurate assessment of adiaspore density and morphology.

Histopathological findings in lungs of hawksbill turtles collected on the coasts of the states of Espírito Santo and Rio de Janeiro, Brazil

ABSTRACT The present work aimed to report the histopathological findings verified in lungs of hawksbill turtles (Eretmochelys imbricata) retrieved from the coasts of the states of Espírito Santo and Rio de Janeiro, Brazil. Between the years 2010 and 2014, 29 E. imbricata individuals were found stranded on the coasts, already dead or dying during treatment. Lung samples of all specimens were collected during necropsies, fixed in 10% neutral buffered formalin, subjected to routine histological processing and classified histomorphologically. The findings revealed that 37.93% of the specimens presented lung lesions. Of these, 90.91% were rescued alive and 9.09% were found dead; 63.63% were females and 36.37% were males. The mean weight was 5.44 Kg and the mean length 39 cm, characterizing young individuals. The animals originated from São Francisco de Itabapoana - RJ, Aracruz - ES, São Mateus - ES, Guarapari - ES, Linhares - ES, Itapemirim - ES, and Anchieta - ES. Macroscopic analysis revealed presence of foam, hyperemia, nodules in the parenchyma, cyst and caseous material. Microscopic examination evidenced heterophilic bronchopneumonia, parasitic granulomatous pneumonia caused by spirorchiids, bacterial granulomatous pneumonia, fungal granulomatous pneumonia, and congestion. It was concluded that juvenile specimens of Eretmochelys imbricata, females and males, originated from the states of Espírito Santo and Rio de Janeiro and found stranded both alive or dead, have significant lung lesions, mainly inflammatory ones, associated or not with infectious agents.