Comparison of Fenugreek Crop Evapotranspiration Measured by a Micro-lysimeter, Field Water Balance Method and Automatic Closed Canopy Chamber

The attempt was to design and develop an automatic closed canopy chamber (ACCC) having dimension of 1.2 m×1.2 m× 1.2 m for crop evapotranspiration measurement by using transparent acrylic sheet of 4 mm thickness. Between two small fans a temperature and relative humidity sensor was used to measure vapor density. The intermediate circuit was developed for making automation system in ACCC. The arduino based coding was developed as per desired logic operation. The top lead of chamber was automatically closed for 2 minutes when inside and outside temperature and relative humidity of ambient air were similar. During measurement mode of ACCC, the two fans were started automatically. After measurement mode, fans were automatically stopped and top lead was opened. The ACCC was calibrated by evaporating mass of water from water filled tray which was placed inside the automatic closed canopy chamber. The validation of the developed ACCC were made using micro-lysimeters (MLS) having size of 0.2 m × 0.2 m × 0.2 m by growing shallow rooted crop like fenugreek. The depth of irrigation was computed based on soil moisture content before irrigation and field capacity. The field testing of ACCC was made by placing chamber in plots of fenugreek crop. The irrigation was applied by drip irrigation as per crop water consumption. The sensor sensed and recorded the instantaneous temperature and relative humidity at 1 second interval for 24 hours. Two sample t-tests were done to compare the data pair of crop evapotranspiration obtained by the MLS inside the ACCC with that of outside the ACCC to ascertain whether there is any effect of the change in micro-climate for a short period of 2 minute on the crop growth physiological processes. Also, the data pair of crop evapotranspiration measured by the MLs, ACCC using the sensor data of temperature-relative humidity were compared and statistically analyzed through t-test. Similarly, the data pair of ETC measured by the FWB (Field Water Balance Method) and ACCC using the sensor data of temperature-relative humidity were also compared and statistically analyzed through t-test. The calibration factor of the ACCC was found as 1.666. The results revealed that there was no significant difference in the crop evapotranspiration measured by the MLs inside and outside ACCC. Also in case of validation and field testing of ACCC, there were no significant difference between the ETC measured by the ACCC, MLs and FWB at 95 percent confidence level. This implies that there are no effects of the change in micro-climate for a short period of 2 minutes in the chamber, on the plant physiological processes. The ETc rate of fenugreek increases as sun rises and reaches the peak after one to two hour from mid-day and then continuously decreases with time. During validation and field testing of ACCC, the fenugreek crop coefficients varied from 0.72 to 1.04 and 0.69 to 1.02 respectively. The developed ACCC is portable as well as more comfortable and cost effective compared to the lysimeter for the measurement of the actual crop evapotranspiration and the crop coefficient.

Technical Note: A combined soil/canopy chamber system for tracing δ¹³C in soil respiration after a ¹³CO₂ canopy pulse labelling

In this study we present a combined soil/canopy chamber system that allows the investigation of carbon flow through the atmosphere-plant-soil system via a 13CO2 canopy labelling approach – especially when using short vegetation such as tree saplings. The developed chamber system clearly separates soil and canopy compartment in order to (a) prevent physical diffusion of 13C tracer into the soil chamber during a 13CO2 canopy pulse labelling (b) study stable isotope processes in soil and canopy individually and independently. In combination with novel laser spectrometry, measuring CO2 (Aerodyne Research Inc.) and H2O (Los Gatos Research Inc.) isotopologue mixing ratios at a rate of 1 Hz, we were able to trace the label transport from leaves to roots in small beech saplings (Fagus sylvatica L.) without interference due to contamination of the soil matrix and/or canopy re-labelling via tracer returning from soil respiration. A very tight coupling between above- (photosynthesis) and belowground (soil respiration) processes was found, where newly assimilated carbon fixed from the 13CO2 atmosphere re-appeared in soil respiration 2 h after it has been photosynthetically fixed. We were able to demonstrate that leaf metabolism acts on substrate for soil respiration on a diurnal timescale, with input of fresh photosynthates during daytime and starch re-mobilisation during nighttime. Long-term fluctuations in the δ13C of soil respiration, as observed under reduced water availability, could not be described by any biological or instrumental mechanism, as they did occur in an atypical ca. 15 hourly rhythm – potential mechanisms driving these fluctuations are hypothesized.