CRISPR-Cas9: Role in Processing of Modular Metabolic Engineered Bio-Based Products

Biogenetic engineering is a significant technology to sensibly manage microbial metabolic product factories. Genome modification methods for efficiently controlling and modifying genes at the genome level have progressed in biogenetic engineering during the last decade. CRISPR is genome editing technology that allows for the modification of organisms’ genomes. CRISPR and its related RNA-guided endonuclease are versatile advanced immune system frameworks for defending against foreign DNA and RNAs. CRISPR is efficient, accessible, and trustworthy genomic modification tool in unparalleled resolution. At present, CRISPR-Cas9 method is expanded to industrially manipulate cells. Metabolically modified organisms are quickly becoming interested in the production of different bio-based components. Here, chapter explore about the control productivity of targeted biomolecules in divergent cells based on the use of different CRISPR-related Cas9.

Statistical Unfolding Approach to Understand Influencing Factors for Taxol Content Variation in High Altitude Himalayan Region

Taxol drugs can be extracted from various species of the taxaceae family. It is an alkaloid (metabolic product) used for the treatment of various types of cancer. Since taxol is a metabolic product, multiple aspects such as edaphic, biochemical, topographic factors need to be assessed in determining the variation in Taxol Content (TC). In this study, both sensor-based hyperspectral reflectance data and absorption-based indices were tested together for the development of an advanced statistical unfolding approach to understand the influencing factors for TC in high altitude Himalayan region. Seriation analysis based on permutation matrix was applied with complete linkage and a multi-fragment heuristic scaling rule along with the common techniques such as Principal Component Analysis (PCA) and correlation to understand the relationship of TC with various factors. This study also tested the newly developed taxol indices to rule out the possibility of overlapping of TC determining bands with the foliar pigment’s wavelengths in the visible region. The result implies that T. wallichiana with a high TC is found more in its natural habitat of deep forest, relating it indirectly to elevation in the case of the montane ecosystem. Taxol is the most varying parameter among the measured variables, followed by hyperspectral Taxol content (TC) indices such as TC 2, TC 5, and carotenoids, which suggests that the indices are well versed to capture variations in TC with elevation.

Effect of 2-(4-chlorophenylthio) ethylamine hydrochloride on lycopene biosynthesis in pepper fruits

Lycopene is an intermediate metabolic product of the capsanthin biosynthesis pathway in pepper fruits and is one of the strongest antioxidants found in plants. During the ripening of pepper fruits, lycopene is almost completely transformed into the downstream metabolic product capsanthin as well as other substances. As a result, lycopene cannot be enriched in ripe pepper fruits; however, the lycopene content can be increased by 2-(4-chlorophenylthio) ethylamine hydrochloride (CPTA) treatment, using the optimal concentration at the optimal development stage of pepper fruits. The current study tested different CPTA concentrations and fruit developmental stages to increase the lycopene content in pepper fruits. The results showed that the lycopene content was significantly enriched in pepper fruits treated with 0.1% CPTA applied at the turning stage. Real-time quantitative polymerase chain reaction analysis showed that CPTA treatment significantly promoted the expression of the upstream genes (Psy and PDS) involved in the anabolic metabolism of lycopene; however, CPTA treatment had a significant inhibitory effect on the downstream gene (Lcyb) of lycopene synthesis. Therefore, in pepper fruits, CPTA inhibits the normal expression of the Lcyb gene downstream of lycopene, thus achieving notable lycopene enrichment.

Pengaruh Berkumur Dengan Larutan Teh Hijau dan Teh Hitam Terhadap pH Saliva

The prevalence of caries in Indonesia has increased by 53.53% from 2013 to 2018. Low salivary pH (potential of Hydrogen) is one of the main causes of dental caries. Acid as metabolic product of oral microorganism can decrease the salivary pH. The purpose of this paper is to determine the effect of gargling with green and black tea solutions on salivary pH. The increasing of salivary pH linear to salivary secretion. Gargling with green tea or black tea is one of the ways that can increase salivary secretion and pH by provides a mechanical and chemical stimulus to the oral cavity. Green tea and black tea contain polyphenols (catechins) which can increase salivary pH. Conclusion is gargling with green tea and black tea solutions can add salivary pH. Gargling with green tea is more effective to increase salivary pH than black tea. Green tea has a higher catechins content than black tea, in order to anti-cariogenic and anti-bacterial properties are more common in green tea.