cometabolic degradation
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2021 ◽  
Vol 217 ◽  
pp. 112206
Author(s):  
Peng Wang ◽  
Fei Li ◽  
Wenmin Wang ◽  
Ruofan Wang ◽  
Yadong Yang ◽  
...  

2021 ◽  
Author(s):  
Young-Cheol Chang ◽  
Venkateswer Reddy Motakatla ◽  
Dubok Choi

Trichloroethene (TCE) and cis-1,2-dichloroethene (cDCE) are volatile organic compounds (VOCs) that are ubiquitous in the environment and causes health concerns. In addition, the non-degradable nature of conventional plastics causes many...


2020 ◽  
Vol 86 (17) ◽  
Author(s):  
Daiyong Deng ◽  
Dung Ngoc Pham ◽  
Fei Li ◽  
Mengyan Li

ABSTRACT Cometabolic degradation plays a prominent role in bioremediation of commingled groundwater contamination (e.g., chlorinated solvents and the solvent stabilizer 1,4-dioxane [dioxane]). In this study, we untangled the diversity and catalytic functions of multicomponent monooxygenases in Azoarcus sp. strain DD4, a Gram-negative propanotroph that is effective in degrading dioxane and 1,1-dichloroethylene (1,1-DCE). Using a combination of knockout mutagenesis and heterologous expression, a toluene monooxygenase (MO) encoded by the tmoABCDEF gene cluster was unequivocally proved to be the key enzyme responsible for the cometabolism of both dioxane and 1,1-DCE. Interestingly, in addition to utilizing toluene as a primary substrate, this toluene MO can also oxidize propane into 1-propanol. Expression of this toluene MO in DD4 appears inducible by both substrates (toluene and propane) and their primary hydroxylation products (m-cresol, p-cresol, and 1-propanol). These findings coherently explain why DD4 can grow on propane and express toluene MO for active cooxidation of dioxane and 1,1-DCE. Furthermore, upregulation of tmo transcription by 1-propanol underlines the implication potential of using 1-propanol as an alternative auxiliary substrate for DD4 bioaugmentation. The discovery of this toluene MO in DD4 and its degradation and induction versatility can lead to broad applications, spanning from environmental remediation and water treatment to biocatalysis in green chemistry. IMPORTANCE Toluene MOs have been well recognized given their robust abilities to degrade a variety of environmental pollutants. Built upon previous research efforts, this study ascertained the untapped capability of a toluene MO in DD4 for effective cooxidation of dioxane and 1,1-DCE, two of the most prevailing yet challenging groundwater contaminants. This report also aligns the induction of a toluene MO with nontoxic and commercially accessible chemicals (e.g., propane and 1-propanol), extending its implications in the field of environmental microbiology and beyond.


Author(s):  
Jan G. Pieters ◽  
John Sanseverino ◽  
Charles L. Wright ◽  
Kevin G. Robinson ◽  
Catherine L. Cheng ◽  
...  

2020 ◽  
Vol 383 ◽  
pp. 121198 ◽  
Author(s):  
Chang Li ◽  
Xu Zhang ◽  
Yin Lu ◽  
Zheng Fan ◽  
Tiecheng Wang ◽  
...  

2019 ◽  
Vol 127 (6) ◽  
pp. 732-737 ◽  
Author(s):  
Saeme Babatabar ◽  
Seyed Morteza Zamir ◽  
Seyed Abbas Shojaosadati ◽  
Bagher Yakhchali ◽  
Ali Babaei Zarch

2019 ◽  
Vol 281 ◽  
pp. 296-302 ◽  
Author(s):  
Meng Xiao ◽  
Honglei Ma ◽  
Meng Sun ◽  
Xiangyang Yin ◽  
Qingmin Feng ◽  
...  

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