genetically encoded sensors
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Author(s):  
Claudia Lodovichi ◽  
Gian Michele Ratto ◽  
Andrew J. Trevelyan ◽  
Daniele Arosio

Author(s):  
Crystian I. Massengill ◽  
Julian Day-Cooney ◽  
Tianyi Mao ◽  
Haining Zhong

2021 ◽  
Vol 220 (4) ◽  
Author(s):  
Carolina dos Santos Passos ◽  
Yun-Seok Choi ◽  
Christopher D. Snow ◽  
Tingting Yao ◽  
Robert E. Cohen

Histone posttranslational modifications (PTMs) are dynamic, context-dependent signals that modulate chromatin structure and function. Ubiquitin (Ub) conjugation to different lysines of histones H2A and H2B is used to regulate diverse processes such as gene silencing, transcriptional elongation, and DNA repair. Despite considerable progress made to elucidate the players and mechanisms involved in histone ubiquitination, there remains a lack of tools to monitor these PTMs, especially in live cells. To address this, we combined an avidity-based strategy with in silico approaches to design sensors for specifically ubiquitinated nucleosomes. By linking Ub-binding domains to nucleosome-binding peptides, we engineered proteins that target H2AK13/15Ub and H2BK120Ub with Kd values from 10−8 to 10−6 M; when fused to fluorescent proteins, they work as PTM sensors in cells. The H2AK13/15Ub-specific sensor, employed to monitor signaling from endogenous DNA damage through the cell cycle, identified and differentiated roles for 53BP1 and BARD1 as mediators of this histone PTM.


2021 ◽  
Author(s):  
Tsvetan Kardashliev ◽  
Alexandra Weingartner ◽  
Elvira Romero ◽  
Ulrich Schwaneberg ◽  
Marco Fraaije ◽  
...  

Genetically encoded biosensors enable efficient high-throughput screening of oxidative enzyme libraries.


Author(s):  
Li Lin ◽  
Smriti Gupta ◽  
W. Sharon Zheng ◽  
Ke Si ◽  
J. Julius Zhu

AbstractNeural communication orchestrates a variety of behaviors, yet despite impressive effort, delineating transmission properties of neuromodulatory communication remains a daunting task due to limitations of available monitoring tools. Recently developed genetically encoded neurotransmitter sensors, when combined with superresolution and deconvolution microscopic techniques, enable the first micro- and nano-scopic visualization of neuromodulatory transmission. Here we introduce this image analysis method by presenting its biophysical foundation, practical solutions, biological validation, and broad applicability. The presentation illustrates how the method resolves fundamental synaptic properties of neuromodulatory transmission, and the new data unveil unexpected fine control and precision of rodent and human neuromodulation. The findings raise the prospect of rapid advances in the understanding of neuromodulatory transmission essential for resolving the physiology or pathogenesis of various behaviors and diseases.


Nano Letters ◽  
2020 ◽  
Vol 20 (6) ◽  
pp. 4073-4083 ◽  
Author(s):  
Paula K. Zhu ◽  
W. Sharon Zheng ◽  
Peng Zhang ◽  
Miao Jing ◽  
Philip M. Borden ◽  
...  

2020 ◽  
Vol 59 (5) ◽  
pp. 1891-1896 ◽  
Author(s):  
Mengyi Xiong ◽  
Zhenglin Yang ◽  
Ryan J. Lake ◽  
Junjie Li ◽  
Shanni Hong ◽  
...  

2019 ◽  
Vol 132 (5) ◽  
pp. 1907-1912
Author(s):  
Mengyi Xiong ◽  
Zhenglin Yang ◽  
Ryan J. Lake ◽  
Junjie Li ◽  
Shanni Hong ◽  
...  

2019 ◽  
Vol 29 ◽  
pp. 55-64 ◽  
Author(s):  
Emilio P. Mottillo ◽  
Huamei Zhang ◽  
Alexander Yang ◽  
Li Zhou ◽  
James G. Granneman

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