Effect of Temperature on 60nm and 100nm Nanosphere Size Standard using Dynamic Light Scattering

It is difficult to use kinetic motion as a measurement tool without temperature affecting the results. In this study, the size of a known nanosphere was used to monitor the effect of temperature towards particle size determination using dynamic light scattering principles. Temperature deviations are always picked up by size measurement using the principle of Brownian motion. The particle size of 60nm and 100nm polystyrene latex nanoparticles in 10mM NaCl solution was measured at four different temperatures set points of 20, 25, 30, 35 and 40°C using dynamic light scattering mechanism. As a result, the size of polystyrene latex nanoparticles was increased with the increases of the temperature. Therefore, for particle size analysis using dynamic light scattering mechanism the temperature of the test must be maintained at 25°C in order to obtain accurate measurement.