Spatial and temporal evolution of imidazolinone-resitant red rice in 'Clearfield' rice cultivations

The objective of this work was to evaluate the distribution of imidazolinone-resistant (IMI-R) red rice (Oryza sativa) populations, the frequency of alleles conferring resistance to IMI, and the adoption of agronomic practices applied to red rice control, across growing seasons and production regions of the state of Rio Grande do Sul (RS), Brazil. In the experiment, 1,008 red rice populations were screened for resistance to IMI, 760 IMI-R red rice plants were genotyped for the acetolactate synthase (ALS) alleles conferring resistance to IMI, and 40 'Clearfield' rice growers were surveyed. IMI-R red rice populations were widespread throughout RS since the 2006/2007 growing season, with a higher initial frequency in the Depressão Central and Fronteira Sul production regions. The occurrence of IMI-R red rice ranged from 1.6 to 3.5 years after 'Clearfield' rice release. Gly654Glu was the most frequent ALS mutation in IMI-R red rice populations, which shows a gene flow from the most used 'Clearfield' rice cultivars to the red rice plants. Crop rotation systems and certified seed were used by only 30% of the surveyed growers of 'Clearfield' rice, with lower percentages in the production regions where IMI-R red rice appeared faster.

Impact of Off-Site Deposition of Glufosinate to Non-Clearfield Rice

Field studies were conducted near Crowley, LA to evaluate the effects of simulated herbicide drift on ‘Cocodrie' rice. Each treatment was made with the spray volume varying proportionally to herbicide dosage based on a spray volume of 234 L ha−1and a glufosinate rate of 493 g ai ha−1. The 6.3%, 31 g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1and the 12.5%, 62 g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. Glufosinate applied at one-tiller, panicle differentiation (PD) growth stage, and boot resulted in crop injury at 7 and 14 d after treatment. At 21 and 28 d after treatment, crop injury was still evident but was less than 10%. Glufosinate applied at one-tiller resulted in plant height reductions of 4 to 6%; however, at harvest, height reductions were 1% or less. Glufosinate applied to rice in the boot stage had lower rice yield in the primary crop, but no difference was observed in the ratoon crop. Harvested seed from the primary crop germinated 7 to 11% less than the nontreated when rice was treated with 31 and 62 g ha−1of glufosinate. Seedling vigor was reduced when treated with 31 and 62 g ha−1of glufosinate.

Utilization of Saflufenacil in a Clearfield® Rice (Oryza sativa) System

Research was conducted in Mississippi in 2012 and 2013 to compare the efficacy of saflufenacil to other broadleaf herbicides applied in mixtures with imazethapyr in a Clearfield rice system. Saflufenacil at 50 g ai ha−1, carfentrazone at 35 g ai ha−1, a prepackaged mixture of halosulfuron plus thifensulfuron at 35 plus 4 g ai ha−1, and a prepackaged mixture of propanil plus thiobencarb at 2,240 plus 2,240 g ai ha−1 were applied in mixture with imazethapyr at 70 g ai ha−1 early-POST (EPOST) to rice in the one- to two-leaf stage or late-POST (LPOST) to rice in the four-leaf to one-tiller stage. No differences in injury among the broadleaf herbicides or between application timings were detected at any evaluation. Imazethapyr combined with propanil plus thiobencarb or saflufenacil provided the greatest control of barnyardgrass 7 and 14 d after treatment (DAT). Hemp sesbania, ivyleaf morningglory, and Palmer amaranth control was greatest and similar for imazethapyr combined with carfentrazone, propanil plus thiobencarb, and saflufenacil; however, rough rice yield was greatest for imazethapyr combined with propanil plus thiobencarb or saflufenacil. Propanil plus thiobencarb or saflufenacil can be used in a Clearfield rice system to achieve optimum weed control and highest rice yields.

Modeling the Simultaneous Evolution of Resistance to ALS- and ACCase-Inhibiting Herbicides in Barnyardgrass (Echinochloa crus-galli) in Clearfield®Rice

Herbicide-resistant barnyardgrass has become widespread in the rice production systems of the midsouthern United States, leaving few effective herbicide options for controlling this weed. The acetolactate synthase (ALS)- and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides remain largely effective in Clearfield®rice production, but strategies need to be developed to protect the long-term utility of these options. A two-trait model was developed to understand simultaneous evolution of resistance in barnyardgrass to the ALS- and ACCase-inhibiting herbicides in Clearfield rice. The model was used to predict resistance under a number of common weed management scenarios across 1,000 hypothetical rice fields in the Mississippi Delta region and answer some key management questions. Under an ALS inhibitor–only program consisting of three annual applications of imidazolinone herbicides (imazethapyr or imazamox) in continuous Clearfield rice, resistance was predicted within 4 yr with 80% risk by year 30. Weed management programs that consisted of ALS- and ACCase-inhibiting herbicides such as fenoxaprop and cyhalofop greatly reduced the risk of ALS-inhibiting herbicide resistance (12% risk by year 30), but there was a considerable risk for ACCase resistance (evolving by year 14 with 13% risk by year 30) and multiple resistance (evolving by year 16 with 11% risk by year 30) to both of these mechanisms of action. A unique insight was that failure to stop using a herbicide soon after resistance evolution can accelerate resistance to the subsequent herbicide option. Further, a strong emphasis on minimizing seedbank size is vital for any successful weed management strategy. Results also demonstrated that diversifying management options is not just adequate, but diversity combined with timely herbicide applications aimed at achieving high efficacy levels possible is imperative.

Reduction of seed production of red rice escapes in clearfield rice

Roguing is a practice used to reduce the seed source of red rice escapes to control in Clearfield-rice areas. However, there is great difficulty in performing it in large and heavily infested rice fields. This objective of this work was to evaluate the effects of the use of imazamox herbicide, applied in different rates and times, on plants of Clearfield-rice and red rice. Four experiments were conducted during the 2007/08 and 2008/09 growing seasons, in completely randomized block design and treatments arranged in factorial design, using three replications per treatment. The treatments had increasing rates of imazamox, application times and rice cultivars. The rice cultivars tested were IRGA 417, IRGA 422 CL, Avaxi CL and Puitá INTA CL. The variables evaluated were the number of panicles m-2, number of grains panicle-1, spikelet sterility in rice and red rice; and, rice grain yield and its components. The imazamox reduced the seed production of red rice escapes in a simulated situation of commercial Clearfield-rice area. The greater percentage reductions were obtained when this herbicide was applied at final formation of the panicle or panicle exertion of the red rice plant escapes to control. The Puitá INTA CL cultivar has high level of resistance to imazamox, independent of rate and application times tested, becoming the only alternative to the use of this practice.

Impact of Drift Rates of Imazethapyr and Low Carrier Volume on Non-Clearfield Rice

Field studies were conducted near Crowley, LA, in 2005 through 2007 to evaluate the effects of simulated herbicide drift on ‘Cocodrie’ rice. Each application was made with the spray volume varying proportionally to herbicide dosage based on a constant spray volume of 234 L ha−1and an imazethapyr rate of 70 g ai ha−1. The 6.3%, 4.4 g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1and the 12.5%, 8.7 g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. An application of imazethapyr at one-tiller, panicle differentiation (PD), and boot resulted in increased crop injury compared with the nontreated rice. The most injury observed occurred on rice treated at the one-tiller timing. Imazethapyr at one-tiller, PD, and boot reduced plant height at harvest and primary and total (primary plus ratoon) crop yield, with the greatest reduction in primary crop yield resulting from imazethapyr applied at boot. Imazethapyr did not affect rice treated at primary crop maturity.

Differential Tolerance of Clearfield Rice Cultivars to Imazamox

Field studies were conducted to compare the response of one inbred (‘CL161’) and two hybrid (‘CLXL729’ and ‘CLXL745’) Clearfield (CL) rice cultivars to imazamox. Imazamox was applied at 44 and 88 g ai ha−1to rice in the panicle initiation (PI) and PI plus 14 d (PI + 14) growth stages and at 44 g ha−1to rice in the midboot growth stage. Maturity of hybrid CL cultivars was delayed following imazamox at 44 g ha−1applied at PI + 14 and midboot. Furthermore, imazamox at 44 g ha−1, applied at midboot, delayed maturity of CLXL745 more than CLXL729. Expressed as a percentage of the weed-free control plots, rough rice yields for CLXL729 were 91% following imazamox at 44 g ha−1applied at PI + 14, 78% following imazamox at 44 g ha−1applied at midboot, and 77% for imazamox at 88 g ha−1applied at PI + 14. Rough rice yield for CLXL745 was 77 to 92% of the control following all imazamox treatments. All imazamox treatments reduced CLXL745 rough rice yield compared with CL161. Rough rice yield, pooled across CL cultivar, varied with imazamox treatment between years, and these differences may have been a consequence of lower temperatures and solar radiation in the first year. Hybrid CL cultivars CLXL729 and CLXL745 were less tolerant than was CL161 when imazamox was applied at nonlabeled rates (88 g ha−1) and/or timings (PI + 14 or midboot). Because of variability in rice growth stages and irregularities in imazamox application in commercial fields, inbred CL cultivars should be planted where an imazamox application will likely be required.