Molecular Modeling and in Silico Characterization of a Pathogenesis-Related Protein-10 (PR10) and its Coding Genes in some Oryza Species

Rice is a major staple food for more than two thirds of the world population. Pathogenesis-related proteins-10 (PR10) have a range of 154 to 163 amino acid with molecular weight ~ 17 kDa. They are acidic and generally intracellular and cytosolic proteins accumulate in plants in response to biotic and abiotic stresses. In the present study, a PR10 gene and its corresponding protein were characterized in O. sativa, O. barthii, O. glaberrima, O. glumipatula, O. meridionalis, O. nivara, O. rufipogon and O. punctata. The results revealed a narrow range of variation at both DNA and protein levels in all examined species except O. glumipatula. The latter showed a relatively obvious structural variation at protein level. Such variation may be beneficial against different types of stress that requires further elucidation to exploit in rice breeding programs.

Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator

A novel PR10 gene (designated as VpPR10) was isolated from Chinese wild Vitis pseudoreticulata W. T. Wang Baihe-35-1. VpPR10 encoded a 159-amino-acid polypeptide with a predicted molecular mass of 17.46 kDa. Sequence alignment showed that VpPR10 had high similarity with the PR10 proteins of other plant species. Genomic DNA gel blot analysis indicated that VpPR10 belonged to a multi-gene family in Chinese wild Vitis. Quantitative real-time PCR analysis exhibited that the transcript level of VpPR10 altered at different times after inoculation with Uncinula necator under natural field conditions. VpPR10 expression began to decrease after 24 h and reached the lowest level after 72 h. Then it began to increase and obtained the normal level after 96 h. After expression in Escherichia coli and purification, recombinant VpPR10 protein was used to immune rabbit to obtain polyclonal antibodies. Immunoblot analysis showed that the expression pattern of VpPR10 protein was similar with that of VpPR10 mRNA.