Analysis of gmo contents as a component in risk-oriented surveillance over food products safety

Control over use of genetically modified products is a vital task within a risk-oriented model for surveillance over food safety products all over the world including the Russian Federation. Our research goal was to examine domestically manufactured food products in order to determine whether they contained certain regulatory sequences typical for genetically modified organisms. We applied polymerase chain reaction with hybridization-fluorescent detection in real time mode to examine 77 food products samples; the task was to determine whether they contained DNA enhancer (E-35S) and promoter (P-35S) of S35 sequence belonging to cauliflower mosaic virus, terminator of nopaline synthase gene from Agrobacterium tumefaciens (T-NOS), 35S enhancer (E-FMV) and promoter (P-FMV) of Figwort mosaic virus, as well as vegetable DNA inducing soya DNA. When analyzing the extracted DNA, we didn’t detect transgenic elements in any samples; however, there were vegetable components reveled in them including 68.8% samples with soya DNA. We established that some sausages were falsified as they contained vegetable elements. In 15.6% cases data on a product structure turned out to be false because soya DNA was not listed on consumer package. Our research on determining soya DNA and transgenic elements in food products indicates that soya ingredients have been added into food products in spite of their absence in relevant documents as recipe components. All the obtained results taken into account, we assume it is necessary to improve control procedures for detecting genetically modified and vegetable components used as ingredients in food products as their falsification can make for changes not only in their consumer properties but also damage consumers’ health.

Analysis of gmo contents as a component in risk-oriented surveillance over food products safety

Control over use of genetically modified products is a vital task within a risk-oriented model for surveillance over food safety products all over the world including the Russian Federation. Our research goal was to examine domestically manufactured food products in order to determine whether they contained certain regulatory sequences typical for genetically modified organisms. We applied polymerase chain reaction with hybridization-fluorescent detection in real time mode to examine 77 food products samples; the task was to determine whether they contained DNA enhancer (E-35S) and promoter (P-35S) of S35 sequence belonging to cauliflower mosaic virus, terminator of nopaline synthase gene from Agrobacterium tumefaciens (T-NOS), 35S enhancer (E-FMV) and promoter (P-FMV) of Figwort mosaic virus, as well as vegetable DNA inducing soya DNA. When analyzing the extracted DNA, we didn’t detect transgenic elements in any samples; however, there were vegetable components reveled in them including 68.8% samples with soya DNA. We established that some sausages were falsified as they contained vegetable elements. In 15.6% cases data on a product structure turned out to be false because soya DNA was not listed on consumer package. Our research on determining soya DNA and transgenic elements in food products indicates that soya ingredients have been added into food products in spite of their absence in relevant documents as recipe components. All the obtained results taken into account, we assume it is necessary to improve control procedures for detecting genetically modified and vegetable components used as ingredients in food products as their falsification can make for changes not only in their consumer properties but also damage consumers’ health.

Rapid Delivery of Foreign Genes into Plants by Direct Rub-Inoculation with Intact Plasmid DNA of a Tomato Bushy Stunt Virus Gene Vector

ABSTRACT Tomato bushy stunt virus (TBSV) cDNA, positioned between a modified cauliflower mosaic virus 35S promoter and the hepatitis delta virus antigenomic ribozyme with a downstream nopaline synthase gene polyadenylation signal, established infections upon rub-inoculation of plants with intact plasmids. Application of this methodology produced a TBSV DNA-based gene vector which yielded readily detectable levels of localized foreign gene expression in inoculated leaves. This is the first demonstration of an infectious DNA from a member of theTombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-inoculation of miniprep DNA onto a variety of plant species.

Induction of Tumors in Anthurium andraeanum by Agrobactemium tumefaciens

A method was devised for infecting Anthurium andraeanum Linden ex André, an economically important ornamental monocot, with Agrobacterium tumefaciens. Tumors were obtained on plant stems 7 to 10 weeks after inoculation with oncogenic A. tumefaciens strains C58 and A281 cultured previously in an induction medium containing 200 μm acetosyringone at pH 5.5. A higher percentage of tumors were formed in vitro on etiolated internodes (32%) than on green leaf (2%) or petiole explants (3%) 4 weeks after inoculation with induced C58. All explants treated with nontumorigenic A. radiobacter or with induction medium alone failed to produce tumors. Chromatograms showed an accumulation of nopaline in internode explant tumors induced with C58. DNA amplification and hybridization studies showed that the DNA from these tumors, but not from noninoculated anthurium tissue, contained sequences homologous to the nopaline synthase gene of A. tumefaciens T-DNA. Chemical names used 3,5-dimethoxy 4-hydroxyacetophenone (acetosyringone).