Thapsigargin, a new inotropic agent, antagonizes action of endothelin-1 in rat atrial cells

In isolated newborn rat atrial cells, thapsigargin induced a slow rise in cytosolic free Ca2+ concentration ([Ca2+]i) (half-maximum effective concentration = 1 microM) that was independent of the presence of external Ca2+. A 5-min treatment of atrial cells with 5 mM caffeine reduced but did not abolish the action of thapsigargin on [Ca2+]i. A first treatment of atrial cells with 10 microM thapsigargin reduced the action of ionomycin on [Ca2+]i. It also antagonized in a noncompetitive manner the Ca(2+)-mobilizing action of 100 nM endothelin-1 (ET-1). The half-maximum concentration for the inhibition by thapsigargin of ET-1 action was 0.2 microM. Thapsigargin had no action on the basal or ET-1 (100 nM)-stimulated production of inositol phosphates. These results suggest that thapsigargin discharges an inositol 1,4,5-trisphosphate-sensitive and caffeine-insensitive intracellular Ca2+ pool distinct from the sarcoplasmic reticulum. In isolated rat left atria, paced at 1 Hz, thapsigargin (10 microM) produced a transient 48% increase in contractility. It did not alter the contractile responses to 1 microM isoproterenol or to 5 mM caffeine. It had no action on postrest potentiation. Thapsigargin (10 microM) almost completely suppressed the positive inotropic action of 100 nM ET-1. It had no action on the transient negative inotropic response to ET-1. These results suggest that most of the positive inotropic effect of ET-1 is linked to its capacity to mobilize an inositol 1,4,5-trisphosphate-sensitive intracellular Ca2+ pool distinct from the sarcoplasmic reticulum.