alkaloid metabolism
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2021 ◽  
Author(s):  
Manish L. Raorane ◽  
Christina Manz ◽  
Sarah Hildebrandt ◽  
Marion Mielke ◽  
Marc Thieme ◽  
...  

Abstract Since the discovery of the anticancer drugs vinblastine and vincristine, Catharanthus roseus has been intensively studied for biosynthesis of several terpene indole alkaloids (TIAs). Due to their low abundance in plant tissues at a simultaneously high demand, modes of production alternative to conventional extraction are mandatory. Plant cell fermentation might become one of these alternatives, yet decades of research have shown limited success to certain product classes, leading to the question, how to preserve the intrinsic ability to produce TIAs (metabolic competence) in cell culture? We used the strategy to use the developmental potency of mature embryos to generate such strains. Two cell strains (C1and C4) from seeds of Catharanthus roseus were found not only to differ morphologically, but also in their metabolic competence. This differential competence became manifest under phytohormone elicitation, but also upon feeding with alkaloid pathway precursors. The more active strain C4 formed larger cell aggregates and was endowed with longer mitochondria. These cellular features were accompanied by higher alkaloid accumulation in response to methyl jasmonate (MeJA) elicitation. The levels of catharanthine could be increased significantly, while the concurrent vindoline branch of the pathway was blocked, such that no bisindole alkaloids were detectable. By feeding vindoline to MeJA elicited C4 cells, vincristine became detectable; however, only to marginal amounts. In conclusion, these results show that cultured cells are not just “de-differentiated”, but can differ in metabolic competence. In addition to elicitation, and precursor feeding, the cellular properties of the “biomatter” are highly relevant for the success of plant cell fermentation.


2021 ◽  
Vol 12 ◽  
Author(s):  
Trinh-Don Nguyen ◽  
Thu-Thuy T. Dang

Plants produce more than 20,000 nitrogen-containing heterocyclic metabolites called alkaloids. These chemicals serve numerous eco-physiological functions in the plants as well as medicines and psychedelic drugs for human for thousands of years, with the anti-cancer agent vinblastine and the painkiller morphine as the best-known examples. Cytochrome P450 monooxygenases (P450s) play a key role in generating the structural variety that underlies this functional diversity of alkaloids. Most alkaloid molecules are heavily oxygenated thanks to P450 enzymes’ activities. Moreover, the formation and re-arrangement of alkaloid scaffolds such as ring formation, expansion, and breakage that contribute to their structural diversity and bioactivity are mainly catalyzed by P450s. The fast-expanding genomics and transcriptomics databases of plants have accelerated the investigation of alkaloid metabolism and many players behind the complexity and uniqueness of alkaloid biosynthetic pathways. Here we discuss recent discoveries of P450s involved in the chemical diversification of alkaloids and how these inform our approaches in understanding plant evolution and producing plant-derived drugs.


Plant Science ◽  
2018 ◽  
Vol 277 ◽  
pp. 166-176 ◽  
Author(s):  
Thi Dao Vu ◽  
Cyril Jousse ◽  
Nathalie Pawlicki-Jullian ◽  
Séverine Schiltz ◽  
Thi Kieu Oanh Nguyen ◽  
...  

2018 ◽  
Vol 74 (a1) ◽  
pp. a272-a272
Author(s):  
Dean E. Lang ◽  
Julien M. Lancaster ◽  
Miguel A. Torres ◽  
Jeremy S. Morris ◽  
Peter J. Facchini ◽  
...  

2016 ◽  
Vol 171 (4) ◽  
pp. 2371-2378
Author(s):  
Inês Carqueijeiro ◽  
Ana Luísa Guimarães ◽  
Sara Bettencourt ◽  
Teresa Martínez-Cortés ◽  
Joana G. Guedes ◽  
...  

2015 ◽  
Vol 10 (5) ◽  
pp. 1934578X1501000
Author(s):  
Yuling Yin ◽  
Riko Katahira ◽  
Hiroshi Ashihara

Accumulation and metabolism of purine alkaloids in leaves of maté ( Ilex paraguariensis) were investigated. In winter, leaves accumulated caffeine but not theobromine, indicating that caffeine is the end product of purine alkaloid synthesis in maté. To elucidate the purine alkaloid metabolism in maté leaves, the metabolic fate of [8-14C]theobromine, [8-14C]theophylline, [8-14C]caffeine and [8-14C] xanthine was investigated in the leaf disks of young and mature leaves. In young maté leaves, significant amounts of theobromine and theophylline were utilized for caffeine biosynthesis, but the conversion was not observed in mature leaves. A small amount of theophylline was converted to theobromine. Practically no caffeine catabolism was detected in maté leaves during a 24 h-incubation. Catabolism of theobromine and theophylline via 3-methylxanthine was observed mainly in mature leaves. Xanthine was catabolised extensively via ureides in both young and mature leaves, but limited amounts are also utilized for the synthesis of theobromine, theophylline and caffeine. Possible pathways for the metabolism of purine alkaloids in maté leaves are discussed.


2015 ◽  
Vol 113 ◽  
pp. 24-32 ◽  
Author(s):  
P.D. Cárdenas ◽  
P.D. Sonawane ◽  
U. Heinig ◽  
S.E. Bocobza ◽  
S. Burdman ◽  
...  

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