Genetic variation, phylogenetic relationship and spatial distribution of ‘Candidatus Phytoplasma ulmi’ strains in Germany

A recent survey in Germany revealed the wide presence of ‘Candidatus Phytoplasma ulmi’ in native elm stands. Accessions were studied for their genetic variability and phylogenetic relationship based on the conserved groEL and the variable imp gene. While the groEL sequences revealed a high intraspecific homology of more than 99%, the homology of the imp gene dropped to 71% between distantly related sequences. Twenty-nine groEL and 74 imp genotypes were distinguished based on polymorphic sites. Phylogenetic analysis of the groEL gene clustered all ‘Ca. P. ulmi’ strains and separated them from related phytoplasmas of the 16SrV group. The inferred phylogeny of the imp gene resulted in a different tree topology and separated the ‘Ca. P. ulmi’ genotypes into two clusters, one closely related to the flavescence dorée phytoplasma strain FD-D (16SrV-D), the other affiliated with the flavescence dorée phytoplasma strains FD-C and FD70 and the alder yellows phytoplasma (16SrV-C). In both phylograms, ‘Ca. P. ulmi’ genotypes from Scots elm trees formed a coherent cluster, while genotypes from European white elms and field elms grouped less strictly. The regional distribution pattern was congruent for some of the groEL and imp genotypes, but a strict linkage for all genotypes was not apparent.

MAIN FACTORS THAT CONTROL THE DISTRIBUTION OF THE BASIC TYPES OF SECONDARY CHANGES OF RESERVOIR ROCKS

Secondary changes in reservoir rocks in the territory of Western Siberia have a regional distribution pattern, and the amount of oil and gas resources, that are in these zones, reaches 30 % of their total number. Part of the objects where the secondary changes are most clearly manifested, has not yet been introduced into the development, what requires a more detailed approach to planning and conducting geological exploration work in perspective areas, where pore space is modified by secondary processes. In this work the main factors that control the distribution of the main types of secondary changes in reservoir rocks were identified: lithofacies, chemical and tectonic.