Rapid AC Electrokinetic Micromixer with Electrically Conductive Sidewalls

We report a quasi T-channel electrokinetics-based micromixer with electrically conductive sidewalls, where the electric field is in the transverse direction of the flow and parallel to the conductivity gradient at the interface between two fluids to be mixed. Mixing results are first compared with another widely studied micromixer configuration, where electrodes are located at the inlet and outlet of the channel with electric field parallel to bulk flow direction but orthogonal to the conductivity gradient at the interface between the two fluids to be mixed. Faster mixing is achieved in the micromixer with conductive sidewalls. Effects of Re numbers, applied AC voltage and frequency, and conductivity ratio of the two fluids to be mixed on mixing results were investigated. The results reveal that the mixing length becomes shorter with low Re number and mixing with increased voltage and decreased frequency. Higher conductivity ratio leads to stronger mixing result. It was also found that, under low conductivity ratio, compared with the case where electrodes are located at the end of the channel, the conductive sidewalls can generate fast mixing at much lower voltage, higher frequency, and lower conductivity ratio. The study of this micromixer could broaden our understanding of electrokinetic phenomena and provide new tools for sample preparation in applications such as organ-on-a-chip where fast mixing is required.

Empirical Conductivity Equation for the Simulation of the Stationary Space Charge Distribution in Polymeric HVDC Cable Insulations

Many processes are involved in the accumulation of space charges within the insulation materials of high voltage direct current (HVDC) cables, e.g., the local electric field, a conductivity gradient inside the insulation, and the injection of charges at both electrodes. An accurate description of the time dependent charge distribution needs to include these effects. Furthermore, using an explicit Euler method for the time integration of a suitably formulated transient model, low time steps are used to resolve fast charge dynamics and to satisfy the Courant–Friedrichs–Lewy (CFL) stability condition. The long lifetime of power cables makes the use of a final stationary charge distribution necessary to assess the reliability of the cable insulations. For an accurate description of the stationary space charge and electric field distribution, an empirical conductivity equation is developed. The bulk conductivity, found in literature, is extended with two sigmoid functions to represent a conductivity gradient near the electrodes. With this extended conductivity equation, accumulated bulk space charges and hetero charges are simulated. New introduced constants to specify the sigmoid functions are determined by space charge measurements, taken from the literature. The measurements indicate accumulated hetero charges in about one quarter of the insulation thickness in the vicinity of both electrodes. The simulation results conform well to published measurements and show an improvement to previously published models, i.e., the developed model shows a good approximation to simulate the stationary bulk and hetero charge distribution.

Electrokinetic Dispersion in Field Amplified Sample Stacking

In this work, we performed an experimental study of electrohydrodynamic effects on the dispersion of sample ions in field amplified sample stacking (FASS). A typical FASS experiment involves a streamwise electrical conductivity gradient collinear to the applied electric field to enhance the sample stacking. Earlier studies on FASS have focused on how the conductivity gradient sets a non-uniform electro-osmotic flow which causes the dispersion. However, the coupling of the electric field with conductivity gradient leads to a destabilizing electric body force and generates unstable flow. This work demonstrates that generated body force influences the dynamics of FASS. We present a scaling analysis to show that at high fields, electrohydrodynamic effects play a vital role in sample dispersion. To justify our scaling arguments, we performed experiments at varied electric fields which shows that at high electric fields maximum concentration enhancement is lowered significantly. To ensure the EHD effects on the dynamics of FASS, we have also performed experiments with suppressed EOF conditions.

Relative electrical conductivity measurements of the rat frontal cortex and the influence on current source density

summaryThe relative electrical conductivity gradient with depth was estimated in the frontal cortex of anaesthetized rats. Current source density (CSD) approximations of field potentials evoked by ventromedial thalamic stimulations with an assumed homogeneous electrical conductivity of the neocortical tissue were compared to those with correction for the estimated conductivity gradient. In spite of the cellular heterogeneity the electrical conductivity of the frontal cortical tissue was found to be fairly homogeneous inside the superficial (layers I through IV) or deep layers (V- VI). The relative conductivity increased twofold at the transition between superficial and deep layers. Regardless of this changes CSD analysis of the field potentials evoked by ventromedial thalamic stimulation revealed negligible differences between estimations ignoring the conductivity and those taking the conductivity into account. No sinks or sources appeared or disappeared. Both CSD approximations revealed: 1) a strong sink in layer I representing most likely summed monosynaptic EPSPs of the ventromedial thalamic afferents; 2) a strong sink in layer VI, probably representing summed disynaptic EPSPs on dendrites of layer VI pyramidal cells, generated by axons of upper layer pyramidal cells; and 3) a sink in lower layer V representing probably threesynaptic summed EPSPs on dendrites of layer V pyramidal cells.