Efficiency of protein concentrate application in the rations of highly productive cows

The association between science and production is gradually developing due to the arrival of new information and discoveries about the composition of feed and the transformation of nutrients in the body of cows. One of the most important links in the formation of animal productivity during lactation is the use of new feed products and feed additives in feeding. The results of scientific and economic experiment on the use of protein concentrate “Agro-Matic” in feeding of highly productive lactating cows of Ayrshire breed are contained the article. The inclusion of protein concentrate “AgroMatic” in the daily rations of lactating cows in the amount of 1,0 and 1,5 kg/head/day increases the level of indigestible protein by 6,1 and 8,9 %, respectively. The use of protein concentrate in the amount of 1,5 kg in cows feeding helps to increase the digestibility of nutrients in rations the quality of milk and the productivity of cows. When feeding protein concentrate in the amount of 1,5 kg the milk productivity of cows during the period of increasing the milk yield was 4297,5 kg of milk, the yield of essential and dispensable amino acids with milk was significantly higher. The yield of milk protein in cows that received protein concentrate in the ration in the amount of 1,0 kg was 137,7 kg, and when using 1,5 kg – 141,4 kg, which was signifi cantly higher than in the control group by 10,2 and 13,6 kg, respectively. The use of protein concentrate “Agro-Matic” in the ration had a benefi cial eff ect on the digestibility of protein. The digestibility of raw protein of the feed mixture with the inclusion of 1,5 kg of protein concentrate was 71,1 % compared to 68,0 % in the basic ration, which are used at the Maysky complex in the “Plemzavod Maysky”

CONTENT OF AMINO ACIDS IN WINTER WHEAT GRAIN DEPENDING ON THE VARIETY

The amino acid content of soft wheat grain was analyzed depending on the variety. It was found that the content of lysine, leucine, phenylalanine, tyrosine and cystine varies the most from the weather conditions of growing season. The total weight of amino acids varies from 14,01 to 17,46 % of grain depending on the variety of soft wheat. The share of indispensable amino acids is 27–29 % of their total mass. However, the sum content of indispensable amino acids varies significantly depending on the variety and line – from 3,81 to 5,10 %. The highest content of phenylalanine (0.55 %), lysine (0.51 %) and leucine (0.47 %) in the grain of soft wheat Panonicus (st) variety was found. The lowest methionine content is 0.1 %. The remaining amino acids ranged from 0,39 to 0,46 %. Among the dispensable amino acids, there is the highest content of glutamine (3,54 %) and proline (1.11 %), and the lowest content of cystine (0.1 %). The variation coefficient of the samples over the years of research was average for the amino acids serine, glutamine, glycine and arginine – low, histidine, asparagine and methionine – medium, and for the rest - high,. It is obvious that the content of amino acids also varies significantly depending on the weather conditions of the growing season of winter wheat. The content of indispensable amino acids in the grain of Kulundinka soft wheat varied from 0.16 to 0.95 %. The content of threonine (0,78 %) and leucine(0,95 %) was the highest, the content of methionine was the lowest – 0.16 %. The content of other indispensable amino acids ranged from 0,50 to 0,74 %. The content of dispensable amino acids ranged 0,22–4,03 %. The highest content was of proline (1,67 %) and glutamine (4,03 %). The content of other dispensable amino acids varied from 0,80 до 1,12 %. It was found that the content of basic amino acids in wheat grain of P 7 soft line varied from 0,09 to 3,92 %. Thus, the content of indispensable amino acids is dominated by threonine, lysine, valine and leucine (0,54–0,78 %). Among the indispensable are proline, asparagine, and glutamine (1,07–3,92 %). The lowest content among indispensable amino acids was characterized by methionine (0.09 %), among dispensable – cystine (0,27 %). The grain of Kulundinka wheat variety and P 7 line obtained by hybridization of Triticum aestivum L. / Triticum spelta L. has the highest number of indispensable amino acids, which are recommended to be used to obtain high-quality grain.

Dispensable Amino Acids, except Glutamine and Proline, Are Ideal Nitrogen Sources for Protein Synthesis in the Presence of Adequate Indispensable Amino Acids in Adult Men

ABSTRACT Background Nutritionally, there is a dietary requirement for indispensable amino acids (IAAs) but also a requirement for nitrogen (N) intake for the de novo synthesis of the dispensable amino acids (DAAs). It has been suggested that there might be a dietary requirement for specific DAAs. Objectives Experiment 1 tested whether 9 of the DAAs (Ala, Arg, Asn, Asp, Gln, Glu, Gly, Pro, Ser) are ideal N sources using the indicator amino acid oxidation (IAAO) technique. Experiment 2 examined whether there is a dietary requirement for Glu in adult men. Methods Seven healthy men (aged 20–24 y) participated in 11 or 2 test diet intakes, in experiment 1 and 2, respectively, in a repeated measures design. In experiment 1, a base diet consisting of the IAA provided at the RDA was compared with test intakes with the base diet plus addition of individual DAAs to meet a 50:50 ratio of IAA:DAA on an N basis. In experiment 2, the diets corresponded to the amino acid pattern present in egg protein, in which all Glu and Gln was present as Glu, or removed, with Ser used to make the diets isonitrogenous. On each study day the IAAO protocol with l-[1-13C]phenylalanine was used to measure whole-body protein synthesis. Results In experiment 1, repeated measures ANOVA with post hoc multiple comparisons showed that 7 of the 9 DAAs (Ala, Arg, Asn, Asp, Glu, Gly, Ser) decreased IAAO significantly (P < 0.05) compared with base IAA diet, the exceptions being Gln and Pro. In experiment 2, a paired t test did not find significant (P > 0.05) differences in the IAAO in response to removal and replacement of Glu intake. Conclusions The results suggest that in healthy men most DAAs are ideal N sources for protein synthesis, in the presence of adequate IAAs, and that endogenous synthesis of Glu is sufficient. Registered clinicaltrials.gov identifier: NCT02009917.

Effects of Industrial Boiling on the Nutritional Profile of Common Octopus (Octopus vulgaris)

Industrial cooking of common octopus (Octopus vulgaris) under well-established procedures is advantageous for current consumers, which demand healthy and convenient food. This work aimed to evaluate the effect of industrial water boiling, without the addition of salt, on the nutritional profile of common octopus. True retentions (TRs) were calculated for essential nutrients and toxic elements. After boiling, the moisture content decreased, resulting in a concentration of other constituents (protein, fat, fatty acids, majority of amino acids, phosphorus, zinc, and iodine). High TRs were obtained for some essential nutrients: 90.2% (eicosapentaenoic acid, EPA), 89.1% (docosahexaenoic acid, DHA), ≥74.6% (indispensable amino acids, IAA), and 86.8% (iodine). In both raw and boiled octopus, polyunsaturated fatty acids (252.2 and 425.1 mg/100 g), leucine (940.1 and 1613.4 mg/100 g), glutamate (1971.5 and 3257.1 mg/100 g), sodium (393.3 and 332.5 mg/100 g), and zinc (12.6 and 16.6 mg/kg) were, respectively, the most abundant fatty acids, IAA, dispensable amino acids, macro, and micro elements. Cadmium, lead, and mercury levels found in boiled octopus were 0.02, 0.10, and 0.08 mg/kg, respectively. The consumption of 150 g (usual portion) of boiled octopus is advisable because it contributes to significant daily intakes of EPA+DHA (>100%), selenium (75.6%), and iodine (12.4%), and 25% of the daily adequate intake of sodium for adults.

DETERMINATION OF AMINO ACID COMPOSITION OF BROСCOLI CABBAGE PROTEIN

A wide range of fresh fruits and vegetables grown in different geographical areas and represented in international trade networks has changed the consumers' approaches to their choice. The determining criterion of choice is the biological value of plant raw materials, is determined by the content of amino acids, vitamins, mineral elements, β-carotene, which play a significant role in ensuring the functioning of the human body. The chemical composition of fruits and vegetables is highly variable and depends on the type, variety of vegetables, agro climatic conditions of their cultivation, storage characteristics and requires study. The study of the amino acid composition and biological value of the protein of the broccoli varieties of French and Dutch selections, which are regionalized in Ukraine, will determine the most promising varieties in order to provide the population with full-fledged proteins of plant origin. The amino acid composition of the protein of the broccoli Parthenon, Belstar F1, Quinta F1, Monaco F1 is regionalized and suitable for cultivation in different geographical regions of Ukraine. Calculation of the amino acidic score and biological value of the broccoli cabbage protein on which the varieties were ranked. 8 indispensable and 8 dispensable amino acids are identified and quantified. It is found that the greatest content of indispensable amino acids is characteristic for cabbage varieties Parthenon (41.95 mg/100 mg protein) and Belstar F1 (42.26 mg/100 mg protein). All the investigated varieties of broccoli cabbage don’t differ significantly in this indicator. A high content of glutamic acid (from 16.27 to 18.43 mg/100 mg protein), aspartic acid (from 9.54 to 10.56 mg/100 mg protein), arginine (from 6.02 to 6.90 mg) is found in the dispensable amino acids in broccoli cabbage varieties/100 mg). The calculated amino acid scores show that the broccoli cabbage protein is a valuable source of isoleucine, methionine, phenylalanine and tryptophan. The score of methionine, tryptophan and isoleucine ranges from 165.1 % to 183.1 %; from 121.0 to 156.0 % and from 115.3 to 127.8 % respectively. Species of broccoli cabbage are identified, the protein of which has the highest biological value. The performed calculations confirm that the highest biological value is possessed by the broccoli cabbage protein of Quinta F1 variety (64.2 %). This variety is the most balanced in its amino acid composition compared to other varieties. The biological value of the protein of the varieties of Monaco F1, the Parthenon is different and amounted to 63.2 % and 63.1 %, respectively. The lowest biological value is found for the protein Belstar F1 – 60.5 %. The research results of the amino acid composition and biological value of the broccoli cabbage protein of varieties bred and grown in different countries will contribute to the expansion of the scientific database on the effect of climatic growing conditions on the biological value of the broccoli cabbage protein. The obtained results can also be useful for specialists in the agrarian sector and the fruit and vegetable industry.