Co-Fermentation of Food Waste and Municipal Sludge from the Saudi Arabian Environment to Improve Lactic Acid Production by Lactobacillus rhamnosus AW3 Isolated from Date Processing Waste

Food waste and municipal sludge were used as the substrates for the biosynthesis of lactic acid in a batch fermentor. The probiotic bacterial strain Lactobacillus rhamnosus AW3 isolated from date processing waste was used to produce lactic acid in a batch fermentor. Co-fermentation enhanced the biosynthesis of lactic acid and decreased substrate inhibition more than mono-substrate fermentation. A maximum yield of 28.4 ± 0.87 g/L of lactic acid was obtained through co-fermentation of food waste and municipal sludge at an optimized ratio of 2:0.5. Lactic acid production was improved by the supplementation of fructose, peptone, and sodium dihydrogen phosphate at pH 5.5 after 48 h fermentation. This production was approximately three-fold higher than that during mono-fermentation of food waste. The tested bacterial strains were obtained from the Microbial Type Culture Collection (MTCC). Lactic acid showed potent antimicrobial activity against pathogenic organisms, such as Bacillus subtilis MTCC 5981 (14 mm), Staphylococcus aureus MTCC 737 (20 mm), Pseudomonas aeruginosa MTCC 424 (24 mm), Enterobacter aerogenes MTCC111 (19 mm), Escherichia coli MTCC 443 (18 mm), Penicillium chrysogenum MTCC 5108 (19 mm), and Aspergillus niger MTCC 282 (19 mm). The antimicrobial properties of lactic acid have significant potential to inhibit the growth of pathogenic bacteria and fungi and improve probiotic properties. The lactic acid extracted from L. rhamnosus AW3 decreased the pH value of soil (p < 0.01) and increased the availability of soil phosphorus (p < 0.01). These findings demonstrate the bioconversion of food waste and municipal sludge into lactic acid, and the recycling of food wastes in urban areas to enhance soil nutrients.

PENGARUH TERAPI SOCIAL SKILLS TRAINING (SST) DENGAN DAN TANPA DIET CASEIN FREE GLUTEN FREE (CFGF) TERHADAP KEMAMPUAN SOSIALISASI ANAK AUTISME

ABSTRAK Jumlah penyandang autis di dunia sekitar 35 juta orang, kisaran 60.000 – 150.000 autis terjadi pada anak usia dibawah 15 tahun yang ditandai dengan adanya gangguan dan keterlambatan dalam bidang kognitif, bahasa, perilaku, komunikasi dan interaksi sosial. Terapi yang diberikan untuk peningkatan kemampuan sosial pada anak autis dilakukan terapi Social Skill Training (SST). Tujuan penelitian ini adalah mengetahui Pengaruh SST dengan dan tanpa diet CFGF terhadap Kemampuan Sosialisasi Anak Autisme (6 -12 Tahun) di SLB Autis YPPA Padang Tahun 2018. Desain penelitian quasi experimental one - group pre - post test. Populasi dalam penelitian ini seluruh anak autis yang berusia 6-12 Tahun dan sampel diambil dengan convenience sampling yang berjumlah 20 orang dengan dan tanpa diet CFGF. Teknik pengolahan data univariat distribusi frekuensi dan central tedensy dan data bivariat dengan Paired T - test. Hasil penelitian terdapat pengaruh SST dengan dan tanpa Diet CFGF terhadap kemampuan sosialisasi anak autis sebelum dan sesudah diberikan terapi. Saran yang dapat disampaikan pada penelitian ini keperawatan agar dapat mendeteksi secara dini tanda gejala yang dialami oleh anak autis sehingga tidak terjadi tanda gejala berat dan melatih kemampuan sosialisasi anak autis dengan terapi SST. Kata Kunci : Kemampuan Sosialisasi, Social Skill Training (SST), CFGF, Anak Autisme EFFECT OF SOCIAL SKILLS TRAINING (SST) TREATMENT WITH AND WITHOUT DIET CFGF ABILITY TO SOCIALIZATION AUTISM CHILDREN ABSTRACT Number of persons with autism in the world range of 60.000 - 150.000 autis moccurredin children aged below 15 years. Therapy was given to improvement of social skills in autistic children Social Skills Training Therapy (SST). The purpose of this study was to determine the ability of Socialization Effects of SST with and without diet CFGF for Autism Children in SLB Autism YPPA Padang 2018. Quasi – experimental design with one - group pre - post test. The population in this study all children with autism aged 6 – 12 year, and samples were taken by convenience sampling 20 people with and without diet CFGF. Univariate date processing techniques while the frequency distribution and central tedensy and Bivariate date processing techniques with paired T - test. Results of research abilities children with autism after therapy SST with and without diet CFGF with social skillsof children with autism. Suggestions can be submittedto the study of nursing in order to detect early signs of the symptoms experienced by children with autism so there is no sign of sever symptoms and train the socialization skills of children with autism with SST therapy. Key Words : Ability socialization, Social Skills Training, CFGF, autistic children

Bivariate Analysis on Autism Therapy using Social Skills Training in SLB YPPA Padang

Number of persons with autism in the world range of 60.000 - 150.000 autism occurred in children aged below 15 years. Therapy was given to improvement of social skills in autistic children Social Skills Training Therapy (SST). The purpose of this study was to determine the ability of Socialization Effects of SST for Autism Children (6 -12 years) in SLB Autism YPPA Padang 2015. Quasi - experimental design with one - group pre - post test. The population in this study all children with autism aged 6-12 year, and samples were taken by convenience sampling 30 people. Univariate date processing techniques while the frequency distribution and central tedensy and Bivariate date processing techniques with paired T - test. Results of research abilities children with autism after therapy SST, and light weight characteristic factors of symptoms associated with social skills of children with autism. Suggestions can be submitted to the study of nursing in order to detect early signs of the symptoms experienced by children with autism so there is no sign of severe symptoms and train the socialization skills of children with autism with SST therapy.