New Recommendations for Robertsonian Translocation Management After Preimplantation Genetic Testing of Structural Rearrangement (PGT-SR) Attempts: What Should Be Done With Mosaic Embryos?

Robertsonian translocation (RT) carriers are phenotypically normal, but they are known to be at increased risk of repeated miscarriages compared with the general population estimated at about 15% of pregnancies, and also resulting in the birth of a child with a mental retardation or congenital anomalies. Preimplantation Genetic Testing (PGT) is therefore a solution for RT carriers. An appropriate probe strategy allows to differentiate balanced embryos, unbalanced embryos, and mosaic embryos. We performed the first comparative analysis between two or three probes FISH strategies to analyze if a probe strategy choice for PGT-SR studies of Robertsonian translocations (RT) influences the fate of embryos? Our investigations present 13 years of experience of PGT for Robertsonian translocation carriers to improve the accuracy of abnormality detections. A deeper analysis of 283 PGT-SR attempts by comparing two strategies of probes highlighted the irrelevance of using a third probe for FISH diagnosis and above all a significant difference of mosaic embryo rates between probe strategies. These findings could be used as new recommendations of Robertsonian translocation management in many laboratories to improve their practices. It could be readily run, less expensive, reliable and accurate. Furthermore, the propounded strategy of mosaic embryo transfer should be considered after a detailed genetic counseling.

A proteomic profile of postpartum cervical repair in mice

A timely and complete uterine cervical tissue repair postpartum is of necessity to prevent obstetrical complications, such as cervicitis, ectropion, hemorrhage, repeated miscarriages or abortions and possibly preterm labor and malignancies. We recently characterized the morphological alterations, as well as changes in angiogenic expression profile in a mice uterine cervix during the immediate postpartum period. Here, we build on this previous study using a proteomic analysis to profile postpartum tissue changes in mice cervix during the same period, the first 48 h of postpartum. The current proteomics data reveal a variable expression of several intermediate filaments, cytoskeletal modulators and proteins with immune and/or wound-healing properties. We conclude that postpartum cervical repair involves a rapid and tightly regulated balance between a host of biological factors, notably between anti- and pro-inflammatory factors, executed by the M1 and M2 macrophage cells, as revealed by proteomics and verified by confocal immunofluorescence. Future studies will assess the suitability of some of the key proteins identified in this study as potential markers for determining the phase of postpartum cervical repair in obstetrical complications, such as cervical lacerations.