Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study

The present study was focused on the identification of multi-resistant bacteria from the WHO priority pathogens list in the samples taken from different stages of the full-scale municipal wastewater treatment plant and receiving water. Additionally, the seasonal variations of the selected multi-resistant pathogens were analyzed in the samples. In order to the aim of the study, the metagenomic DNA from the collected samples was isolated and sequenced. The samples were collected in three campaigns (spring, summer, autumn). Metagenomic DNA was isolated by the commercial kits, according to the manufacturer’s instruction. Illumina sequencing system was employed, and the R program was used to metagenomic analysis. It was found that the wastewater samples and receiving water contained the multi-resistant bacteria from the WHO priority pathogens list. The seasonal and technological variations affected the distribution of the pathogens in the wastewater. No effect of the effluent on the pathogens in the receiving water was observed. The results indicated that antibiotic-resistant “priority pathogens” from the WHO list are there in the waste- and receiving water. Technological process and seasons effected their distribution in the environment. Metagenomic analysis can be used as sufficient tool in microbiological and human health risk assessment.

Investigating the Development of Individuals with Mental and Physical Problems Receiving Water Therapy in a Special Education and Rehabilitation Center

Investigation of the physical and psychological development challenged individuals receiving water therapy in a private education rehabilitation center. The aim of this study is to investigate the physical and psychological development challenged individuals receiving water therapy in a private education rehabilitation center.5 children with mentally and physically problems who took water therapy in the Special Education Rehabilitation Center participated in the study. Children aged between 3-8. Children who have mentally problematic, who have participated in the research, have severe, moderate and mild mental problems and who have problems physically also have placenta, quatri plaque, spina bifida etc. children with orthopedic insufficiency.Special mobility training program was prepared for the children to be able to make joint problems for children with mental problems and physical problems. While the program was being prepared, support was received from special educator, water therapist and repertoire people.The study was administered 4 months, 2 days a week and 60-90 minutes. At the beginning of the research, the children who had mental problems were afraid of entering the water, they did not comply with the commands, they did not want to be separated from the mother and father.At the end of the research process, children with mental problems enter the water comfortably and even hurry to enter, they are not afraid of water, they are more adaptable to the commands, they continue to work separately from their parents, they are more willing to enter the water in children who have problems with orthopedic problems. It was observed that most of the children who wanted to move in the water, they were mostly able to perform the movements, and the majority of the children (90%) who had walking pants had walking exercises.

Process evaluation of urban river replenished with reclaimed water from a wastewater treatment plant based on the risk of algal bloom and comprehensive acute toxicity

Municipal wastewater reuse has an important role to play with scarce water resources and serious water pollution. However, the impact of reclaimed water on the aquatic ecology and organisms of the receiving water needs to be assessed. This study investigated one ecological restoration project of an urban river replenished with reclaimed water, and evaluated the risk of algal bloom and acute biological toxicity in the river. Results showed that the concentrations of permanganate index and ammonia nitrogen in the river could stably remain below the standard values, the concentrations of total phosphorus were high and most of the monitoring values were between 0.42 and 0.86 mg/L. The content of chlorophyll a was relatively lower, ranging from 0.06 to 0.10 mg/m3. The maximum value of Fv/Fm was 0.42, which was lower than the algal bloom prediction threshold of 0.63. Moreover, the results of luminescence inhibition rate on luminescent bacteria showed that the reclaimed water did not cause significant biological toxicity to the aquatic ecology. The study suggested that implementing urban reclaimed water reuse projects requires a series of ecological purification and restoration technologies in the receiving water, which can effectively guarantee the stability of water quality and the safety of water ecological environment.

Zero-Emission of Palm Oil Mill Effluent Final Discharge Promoted Bacterial Biodiversity Rebound in the Receiving Water System

Zero-emission technology for palm oil mill effluent (POME) has led to a breakthrough in the palm oil industry in relation to the goal of sustainable development. However, there are limited resources on how this technology has affected the bacterial community in the receiving river that has previously been polluted with POME final discharge. Thus, the current study assessed the recoverability of the unexplored bacterial community in the receiving water of a constructed river water system post-zero emission of POME final discharge. An artificial river water system was constructed in this study, where the viability status and the composition of the bacterial community were assessed for 15 days using a flow cytometry-based assay and high-throughput sequencing by Illumina MiSeq, respectively. The zero-emission of POME final discharge reduced not only the physicochemical properties and nutrient contents of the receiving water, but also the bacterial cells’ viability from 40.3% to 24.5% and shifted the high nucleic acid (HNA) to low nucleic acid (LNA) content (38.7% to 34.5%). The proposed POME bacterial indicators, Alcaligenaceae and Chromatiaceae were not detectable in the rainwater (control) but were detected in the artificial river water system after the introduction of POME final discharge at the compositions of 1.0–1.3% and 2.2–5.1%, respectively. The implementation of a zero-emission system decreased the composition of Chromatiaceae from 2.2% on day 8 until it was undetectable on day 15, while Alcaligenaceae was continuously reduced from 1.2% to 0.9% within that similar time frame. As indicated by principal coordinate (PCO) analysis, the reductions in biological oxygen demand (BOD5) would further diminish the compositions of these bioindicators. The zero-emission of POME final discharge has demonstrated its efficacy, not only in reducing the polluting properties, but also in the bacterial biodiversity rebound in the affected water system.

Mutagenicity evaluation to UV filters of benzophenone-6, benzophenone-8, and 4-methylbenzylidene camphor by Ames test

Benzophenone (BPs) and 4-Methylbenzylidene Camphor are used as ultraviolet (UV) filters to protect the skin and hair in personal care products. The discharging of the three chemicals may endanger the receiving water ecosystem. In the present study, the mutagenicity of BP-6, BP-8, and 4-Methylbenzylidene Camphor was tested using the Salmonella typhimurium reverse mutation test (Ames test) in the system with and without rat liver microsomal preparations (S9). Four S.typhimurium strains, TA97, TA98, TA100, and TA102 were employed in the Ames tests. The mutagenicity was detected from all three chemicals. The addition of S9 increased the mutation ratios of three chemicals to four strains, except BP-6 to TA100 strain and 4-MBC to TA97 and TA98 strain. In the mixed experiment, all positive effects were detected in the absence of S9. However, the results all became negative in the presence of S9. For the mixture of BP-6 and 4-MBC, positive results were detected on four tester strains except for the TA100 strain. For the mixture of BP-6, BP-8, and 4-MBC, positive results were detected on four strains. The mixture test results showed antagonism in mutagenicity for the mixture of BP-6 and 4-MBC to TA98 and TA100 strains and the mixture of BP-6, BP-8, and 4-MBC to TA100 and TA102 strains.

Modeling Tidal Characteristics in a Creek-Marsh Drainage System: Implications for Stormwater Management

The traditional goal of stormwater management is to reduce the threat of flooding to life and property, and so most landscapes are engineered to maximize the speed at which the unwanted water leaves the watershed. This has been effective in landscapes with some topographic gradient. This often involves the installation of drainage ditches that disperse runoff from urban areas to receiving water bodies; in coastal areas this means a tidal creek, estuary, bay, sounds, or the coastal ocean. This practice reduces flood hazards in some cases but results in unintended effects on the natural hydrology in the watershed and downstream tidal dynamics. For low-gradient watersheds in humid climates, ditch systems also lower the water table of an area, increasing infiltration to recharge and groundwater discharge to streams (baseflow), and larger volume of freshwater delivered downstream yearround. Ditches also create unintentional avenues for the incoming tide from a tidal creek or tidally-influenced waterway to reach further inland, thus reducing the hydraulic gradient between the inland areas and the receiving water body. The combination of these effects can exacerbate compound flooding events, increasing the flood probability if high tide and storm events coincide. Additionally, coastal communities face the challenge of mitigating more complicated flood hazards while land development increases to meet the needs of a growing population. This study analyzed the tidal influence within an inland drainage ditch in the central coast of South Carolina USA that is representative of thousands of artificially-drained coastal watersheds. The ditch-creek system investigated here is 12 km long in a 753-hectare (1860-acre) watershed of Church Flats Creek, a first-order tidal system. We monitored for 13 months a 0.75-km reach of the lower ditch portion of the system, just above the relatively undisturbed tidal creek and marsh. Prior to ditching in the 1960s this system had a wetland-rich floodplain but is now partially tidal. Field data collected were stream stage (depth), discharge, tidal range, tidal volume, incoming (flood) and outgoing (ebb) tidal durations, and water table hydrograph at a location about 50 m of mid-reach of the ditch. Multiple linear regressions were performed to best predict the flood and ebb tidal durations of the system based on tidal characteristics within the ditch. The mean values were 229 ± 2.5 and 182 ± 2.1 minutes for flood and ebb tide durations, respectively and the models explained 84% (residual standard error (RSE) of 25 minutes) and 80% (RSE of 23 minutes) for the flood and ebb conditions, respectively. The models were simulated for sea levels in 1993 and 2050, and results indicate that the flood tide within the drainage ditch is predicted to increase an average of 66 minutes and the total tidal duration (flood and ebb) an average of 139 minutes by 2050. These results suggest a loss in drainage functionality as sea level rises. Increases in the duration of tidal influence will induce a lower capacity for stormwater volume than the drainage infrastructure was constructed to manage, therefore resulting in an increased frequency of compound flooding events because of the lower storage volume and decreased hydraulic gradient in the system. This study fills a knowledge gap of tidal dynamics within coastal ditch-creek systems and we urge stormwater managers to consider the unintended consequences of using traditional stormwater methods in a region that does not benefit from gravity drainage practices like in other regions.

Deltas: a new classification expanding Bates’s concepts

Deltas constitute complex depositional systems formed when a land-derived gravity-flow (carrying water and sediments) discharges into a marine or lacustrine standing body of water. However, the complexity of deltaic sedimentary environments has been oversimplified by geoscientists over the years, considering just littoral deltas as the unique possible type of delta in natural systems. Nevertheless, a rational analysis suggests that deltas can be much more complex. In fact, the characteristics of deltaic deposits will depend on a complex interplay between the bulk density of the incoming flow and the salinity of the receiving water body. This paper explores the natural conditions of deltaic sedimentation according to different density contrasts. The rational analysis of deltaic systems allows to recognize three main fields for deltaic sedimentation, corresponding to (1) hypopycnal (2) homopycnal and (3) hyperpycnal delta settings. The hypopycnal delta field represents the situation when the bulk density of the incoming flow is lower than the density of the water in the basin. According to the salinity of the receiving water body, three different types of hypopycnal littoral deltas are recognized: hypersaline littoral deltas (HSLD), marine littoral deltas (MLD), and brackish littoral deltas (BLD). The basin salinity will determine the capacity of the delta for producing effective buoyant plumes, and consequently the characteristics and extension of prodelta deposits. Homopycnal littoral deltas (HOLD) form when the density of the incoming flow is roughly similar to the density of the water in the receiving basin. This situation is typical of clean bedload-dominated rivers entering freshwater lakes. Delta front deposits are dominated by sediment avalanches. Typical fallout prodelta deposits are absent or poorly developed since no buoyant plumes are generated. Hyperpycnal deltas form when the bulk density of the incoming flow is higher than the density of the water in the receiving basin. The interaction between flow type, flow density (due to the concentration of suspended sediments) and basin salinity defines three types of deltas, corresponding to hyperpycnal littoral deltas (HLD), hyperpycnal subaqueous deltas (HSD), and hyperpycnal fan deltas (HFD). Hyperpycnal littoral deltas are low-gradient shallow-water deltas formed when dirty rivers enter into brackish or normal-salinity marine basins, typically in wave or tide-dominated epicontinental seas or brackish lakes. Hyperpycnal subaqueous deltas represent the most common type of hyperpycnal delta, with channels and lobes generated in marine and lacustrine settings during long-lasting sediment-laden river-flood discharges. Finally, hyperpycnal fan deltas are subaqueous delta systems generated on high-gradient lacustrine or marine settings by episodic high-density fluvial discharges.