human bcl6
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2021 ◽  
Vol 22 (22) ◽  
pp. 12400
Author(s):  
Marat Sabirov ◽  
Anastasia Popovich ◽  
Konstantin Boyko ◽  
Alena Nikolaeva ◽  
Olga Kyrchanova ◽  
...  

Most of the known Drosophila architectural proteins interact with an important cofactor, CP190, that contains three domains (BTB, M, and D) that are involved in protein–protein interactions. The highly conserved N-terminal CP190 BTB domain forms a stable homodimer that interacts with unstructured regions in the three best-characterized architectural proteins: dCTCF, Su(Hw), and Pita. Here, we identified two new CP190 partners, CG4730 and CG31365, that interact with the BTB domain. The CP190 BTB resembles the previously characterized human BCL6 BTB domain, which uses its hydrophobic groove to specifically associate with unstructured regions of several transcriptional repressors. Using GST pull-down and yeast two-hybrid assays, we demonstrated that mutations in the hydrophobic groove strongly affect the affinity of CP190 BTB for the architectural proteins. In the yeast two-hybrid assay, we found that architectural proteins use various mechanisms to improve the efficiency of interaction with CP190. Pita and Su(Hw) have two unstructured regions that appear to simultaneously interact with hydrophobic grooves in the BTB dimer. In dCTCF and CG31365, two adjacent regions interact simultaneously with the hydrophobic groove of the BTB and the M domain of CP190. Finally, CG4730 interacts with the BTB, M, and D domains of CP190 simultaneously. These results suggest that architectural proteins use different mechanisms to increase the efficiency of interaction with CP190.


PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0216470
Author(s):  
Kunzhi Jia ◽  
Danping Zhang ◽  
Yanghai Wang ◽  
Yaju Liu ◽  
Xiangzhu Kong ◽  
...  

2014 ◽  
Vol 52 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Beverly W. Baron ◽  
John Anastasi ◽  
Juraj Bies ◽  
Poluru L. Reddy ◽  
Loren Joseph ◽  
...  
Keyword(s):  

2012 ◽  
Vol 109 (15) ◽  
pp. 5735-5739 ◽  
Author(s):  
B. W. Baron ◽  
J. Anastasi ◽  
E. M. Hyjek ◽  
J. Bies ◽  
P. L. Reddy ◽  
...  
Keyword(s):  

Author(s):  
Mark A. Stead ◽  
Gareth O. Rosbrook ◽  
Jonathan M. Hadden ◽  
Chi H. Trinh ◽  
Stephen B. Carr ◽  
...  
Keyword(s):  

2004 ◽  
Vol 101 (39) ◽  
pp. 14198-14203 ◽  
Author(s):  
B. W. Baron ◽  
J. Anastasi ◽  
A. Montag ◽  
D. Huo ◽  
R. M. Baron ◽  
...  
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