Development of Local Functional Food Made of Buffalo Milk Improved with Culture Probiotics (Lactobacillus casei)

The research was conducted to develop functional food products of milk-based livestock origin (Semi hard-type cheese), with the addition of pure culture Lactobacillus casei as a probiotic agent, and citric acid and Mucor meihei as milk coagulants. The research material was semi-hard type cheese made of approximately 35 liters of buffalo milk from West Sumbawa Regency as a basic ingredient with the probiotic pure culture. The results showed that the pure culture of probiotic (Lactobacillus casei) at levels of 10% and 15% can survive and develop quite well in semi-hard cheese during aging, from 1 day, 7 days, and 14 days, respectively (3.79 – 5.92) and (4.91 – 6.31) log cfu g-1. While the 0.025% rennet of the volume of milk from Mucor miehei gives a pretty good result, it can be seen from the product recovery which can reach (34.30 + 0.32) %. During aging for 14 days, an organoleptic quality which includes aroma, color, and texture was getting better, with the criteria of a semi-hard aroma, yellowish-white color, and semi-hard texture. It can be concluded, that semi-hard type cheese can be used as one of the functional foods of probiotic carriers. To get the therapeutic effect, this probiotic should be consumed at least 100 grams per serving.

Hygrophorus aurantiosquamosus (Hygrophoraceae, Agaricales), a new species of Hygrophorus section Chrysodontes from western China

In China, the European species, Hygrophorus chrysodon, is widely reported in western and northeastern provinces. After carefully comparing H. chrysodon from China and Europe, we found that the European and Chinese materials were different lineages in molecular trees, and there were also obvious differences in morphology between the two lineages. The evidence from morphology and phylogeny indicated that the presence of H. chrysodon in China is doubtful and that the Chinese material represents an undescribed species. Thus, H. aurantiosquamosus is proposed as a new species. This new taxon is characterized by its yellowish white pileus covered with golden yellow squamules, a stipe concolorous with the pileus and covered with yellow floccules, a distinct pileipellis composed of narrow hyphae with inflated terminal elements of various shapes (clavate, cylindrical, lacrymoid to subglobose), and elongate to subcylindrical basidiospores measuring 9.5–12 × 4–5.5 μm. The new species occurs in Picea forests at high elevations of western China, such as Qinghai, Sichuan, and Tibet provinces.

INTERSTITIAL RENAL NECROSIS SYNDROM OF CALVES

Interstitial renal necrosis syndrom (IRNS) was diagnosed in calves sloughtered in Mosul sloughter house. Gross and microscopical picture of 33 affected animals were described. The lesions were identified according topathomorpholgy of tissue reaction. Two patterns of tissue rections were observed. The lesions of the first pattern showed grossly an oval, greyish white translucent nodules on the surface of kidney. Microscopically, cells like lymphocytes and few plasma cells were aggregated in the interstitial tissue. The lesions of the second pattern comprised grossly a milliary yellowish-white nodules on the surface of the kidney and on cut sectionforming extention in the depth of the renal cortex. Microscopically, the infiltrated cells were mainly neutrophils, macrophages and few lymphocytes, the possible causes of this syndrom was discussed.

Two new species of Phallus (Phallaceae) with a white indusium from China

Two new Phallus species, P. cremeo-ochraceus and P. rigidiindusiatus were discovered in southwestern and southern China, respectively. Phallus cremeo-ochraceus is morphologically characterized by its cream to ochraceous receptacle, white to very slightly pinkish indusium, white to pinkish pseudostipe and white to slightly purplish pink volva. Phallus rigidiindusiatus is characterized by a white to yellowish white receptacle, a strongly rigid indusium usually without serrated margin and smaller basidiospores than those of P. serratus. Phylogenetic positions of the two species are located in two independent lineages respectively. Detailed descriptions, color photographs, illustrations and a key to the related species are presented.

Effect of the Addition Tilapia Skin Collagen Concentration to Lotion Characteristics

The purpose of this research was to determine the best concentration of the tilapia skin collagen addition to obtain the characteristics of the lotion in accordance with the established standards. The research was carried out at the Fishery Product Processing Technology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Central Laboratory of Padjadjaran University and Faculty of Mathematics and Natural Sciences Laboratory of Padjadjaran University between March 2021 - June 2021. The method used in this research was experimental with a completely randomized design consisted of 5 treatments with 4 replications added the concentration of the tilapia skin collagen (0%, 4%, 5%, 6% and 7%) based on lotion formulations. Parameters observed included pH, viscosity, spreadability, weight loss, homogeneity and the organoleptic (appearance, color, scent, texture). The conclusion from this research was that the addition of 7% the tilapia skin collagen concentration was the best treatment with a homogeneous appearance, a slightly yellowish white color, a slightly lavender scent and a slightly thick texture. The lotion had pH value of 6.35, viscosity of 7,197 cP, 5.74 cm spreadability and a weight loss of 2.8%.

Amomum xizangense (Zingiberaceae), a new species from Xizang, China

Amomum xizangense (Zingiberaceae), a new species from Xizang, China, is described and illustrated. It is similar to A. maximum and A. subcapitatum, but differs in having purple-red leaf sheath, purple-red or reddish-green petiole, lamina with purple-red midvein, persistent bracts and bracteoles, yellowish-white calyx, yellowish corolla, yellow anther with trilobed and yellow crest and globose fruit.

Morphology and molecular phylogeny reveal two new species in Russula sect. Ingratae from China

Two new species of Russula are described and illustrated in this paper. Russula clavulus is recognised by a pale yellow pileus centre, white margin with tuberculate striation, white to pale lamellae with small pale yellow spots, white to light yellow spore print, subglobose to broadly ellipsoid spores with short or long ridges and hymenial cystidia on lamellae sides that are mainly subclavate or fusiform. Russula multilamellula is morphologically characterised by the brownish orange to hazel pileus centre and satin white to yellowish-white margin with brownish tinge, lamellulae that are usually irregular in length and often anastomosing with lamellae, subglobose to broadly ellipsoid spores with short or long ridges and clavate hymenial cystidia. The combination of morphological features and multigene phylogenetic analyses of ITS-nrLSU-RPB2-mtSSU data indicated that these two new taxa belong to Russula subg. Heterophyllidia sect. Ingratae.

Characteristics of Composite Flour (Biang fish Ilisha elongata and Sago) For the Development of Good Nutritional Food Products

Composite flour is a mixture of fish flour and sago flour to increase nutrition in food products. This study aims to increase the nutritional value of food products with sago flour as raw material. Composite flour from fish and sago flour were 0%, 2%, 4%, 6%, 8%, and 10% Biang fish to the amount of sago flour. The results of the research on fish flour Ilisha elongata showed a yellowish-white color with a whiteness degree of 75.8%. Fish flour has a yield of 28.9% with a fineness level on a 100-mesh sieve. The results show the characteristics of the composite flour of Biang fish and sago, respectively, the color of the flour was white-gray to cream color; having nutritional characteristics, respectively, the moisture content is 9.62%; 9.57%; 9.55%; 9.42%; 9.26%, and 9.20%. The protein content was 0.25%; 3.58%; 4.09%; 4.99%; 5.98%, and 6.39%. The fat content was 0.41%, 0.24%, 0.29%; 0.37%; 0.40%; 0.42%. The ash contained 0.27%, 0.34%, 0.50%, 0.57%, 0.69%, and 0.76%. The carbohydrates were 89.45%; 86.27%; 85.57%; 84.65%; 83.67%, and 83.23%. The calcium content was 1130 mg/kg; 1901 mg/kg; 2687 mg/kg; 2770 mg/kg; 2827 mg/kg and 2869 mg/kg. The addition of fish flour can increase the nutritional value of composite flour made from sago flour and has the potential to develop nutritious food products.

A new species of the Genus Pelodiscus Fitzinger, 1835 (Testudines: Trionychidae) from Huangshan, Anhui, China

A new species of the soft-shelled turtle genus Pelodiscus is described based on seven specimens from Huangshan, southern Anhui Province, China. The new species, Pelodiscus huangshanensis sp. nov., is distinguished from other species in the genus Pelodiscus by the following characteristics: (1) Small size (maximum carapace length of 101.16 mm and maximum body length of 190 mm); (2) keel high; (3) tiny yellowish-white spots on the throat; (4) no black pinstripes around the eyes; (5) white longitudinal bands on both sides of the neck in juveniles, absent in adults; (6) plastron yellowish-white, and only a dark patch on each side of the armpit; (7) many tubercles on the dorsal surface, but indistinct in the center; and (8) entoplastron “⌒” shaped. The phylogenetic relationships of the species in Pelodiscus were reconstructed using the sequences of cytochrome b (cyt b) and NADH dehydrogenase subunit 4 (ND4) genes. The new species formed a monophyletic clade with strong support. The uncorrected pairwise distances between the new species and other representatives of Pelodiscus ranged from 5.4% to 9.2% for cyt b and 4.1% to 7.6% for ND4. The new species brings the number of species of the genus Pelodiscus to six; five species are distributed in China, with three species endemic to China.

Evaluation of Native Rooster Based on Breeding Value of trait of Macroscopic and Microscopic Semen Quality

The aim of this research is to a determined the breeding value of native roosters based on macroscopic and microscopic semen. Ten native roosters were used in this research. Complete individual cage, semen collection tools and substance, macroscopic and microscopic tools, and substance were used in this research. The native rooster was rested for 2 weeks. Massage techniques were used for semen collection. Parameters observed were volume, pH, color, viscosity, smell, sperm concentration, and sperm livability. The semen collection was repeated 5 times. One-way analysis of variances was used for data analysis with the tools SPSS 16. The breeding value was estimated based on primary traits of semen and calculated for each rooster to make a rank. The results showed that significant parameters (P<0,05) are semen volume and sperm concentration, while the non-significant parameters (P?0,05) are pH semen and sperm livability. Semen color is yellowish-white, semen viscosity is medium-viscous and smell is spermin. The results of breeding value rank are rooster numbers 6, 7, 5, 3, 2, 4, 8, 9, 10, and 1. The conclusion is the breeding value of Native roosters is varied. The recommendation is Native chicken breeding need to apply selection used breeding value based on semen quality.