Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi

This is the first study in an aquatic ectotherm to combine a stoichiometric bioenergetic approach with an endpoint stochastic model to explore dietary macronutrient content. The combination of measuring respiratory gas (O2 and CO2) exchange, nitrogenous (ammonia and urea) excretion, specific dynamic action (SDA), metabolic energy substrate use, and whole-body protein synthesis in spiny lobster, Sagmariasus verreauxi, was examined in relation to dietary protein. Three isoenergetic feeds were formulated with varying crude protein: 40%, 50% and 60%, corresponding to CP40, CP50 and CP60 treatments, respectively. Total CO2 and ammonia excretion, SDA magnitude and coefficient, and protein synthesis in the CP60 treatment were higher compared to the CP40 treatment. These differences demonstrate dietary protein influences post-prandial energy metabolism. Metabolic use of each major energy substrate varied at different post-prandial times, indicating suitable amounts of high-quality protein with major non-protein energy-yielding nutrients, lipid and carbohydrate, are critical for lobsters. The average contribution of protein oxidation was lowest in the CP50 treatment, suggesting mechanisms underlying the most efficient retention of dietary protein and suitable dietary inclusion. This study advances understanding of how deficient and surplus dietary protein affects energy metabolism and provides approaches for fine-scale feed evaluation to support sustainable aquaculture.

Mismatch of thermal optima between performance measures, life stages and species of spiny lobster

In an ocean warming hotspot off south-east Australia, many species have expanded their ranges polewards, including the eastern rock lobster, Sagmariasus verreauxi. This species is likely extending its range via larval advection into Tasmanian coastal waters, which are occupied by the more commercially important southern rock lobster, Jasus edwardsii. Here, thermal tolerances of these lobster species at two life stages were investigated to assess how they may respond to warming ocean temperatures. We found that the pattern, optimum and magnitude of thermal responses differed between performance measures, life stages and species. Sagmariasus verreauxi had a warmer optimal temperature for aerobic scope and escape speed than J. edwardsii. However, J. edwardsii had a higher magnitude of escape speed, indicating higher capacity for escape performance. There were also differences between life stages within species, with the larval stage having higher variation in optimal temperatures between measures than juveniles. This inconsistency in performance optima and magnitude indicates that single performance measures at single life stages are unlikely to accurately predict whole animal performance in terms of life-time survival and fitness. However, combined results of this study suggest that with continued ocean warming, S. verreauxi is likely to continue to extend its distribution polewards and increase in abundance in Tasmania.

Molecular characterization and expression analysis of the inverbrate Dmrt1 homologs in the swimming crab, Portunus trituberculatus (Miers, 1876) (Decapoda, Portunidae)

The doublesex/mab-3 related transcription factor (Dmrt) gene family plays a conserved sexual regulatory role in different phyla, thus providing an important clue for studying the sex mechanism of crustaceans. In this study, cDNAs of three Dmrt homologs were cloned and characterized in the swimming crab, Portunus trituberculatus (Miers, 1876). Sequence analysis indicated the obtained Dmrts might be orthologs of the invertebrate Dmrt1 (iDmrt1) described in the spiny lobster, Sagmariasus verreauxi (H. Milne-Edwards, 1851). Real-time quantitative PCR revealed that the PtiDmrt1 transcripts were highly expressed in testis, muscle, Y-organ, and gill, and in the C2 stage during the developmental process. In addition, RNAi of PtiDmrt1 showed that the gene expression of insulin-like androgenic gland hormone (IAG) decreased remarkably at 24 h after injection, but increased to a higher level than in the control group at 48 h post injection. The results suggested that the PtiDmrt1 is a potential regulator of IAG, although the underlying mechanism may be complicated.

Gonadulins, the fourth type of Insulin-related peptides in Decapods

Insulin and related peptides play important roles in the regulation of growth and reproduction. Until recently three different types of insulin-related peptides had been identified from decapod crustaceans. The identification of two novel insulin-related peptides from Sagmariasus verreauxi and Cherax quadricarinatus suggested that there might a fourth type. Publicly available short read archives show that orthologs of these peptides are commonly present in these animals. Most decapods have two genes coding such peptides, but Penaeus species have likely only one and some palaemonids have three. Interestingly, expression levels can vary more than thousand-fold in the gonads of Portunus trituberculatus, where gonadulin 1 is expressed by the testis and gonadulin 2 by the ovary. Although these peptides are also expressed in other tissues, the occasionally very high expression in the gonads led to them being called gonadulins.