A Hybrid Microbial–Enzymatic Fuel Cell Cathode Overcomes Enzyme Inactivation Limits in Biological Fuel Cells

The construction of optimized biological fuel cells requires a cathode which combines the longevity of a microbial catalyst with the current density of an enzymatic catalyst. Laccase-secreting fungi were grown directly on the cathode of a biological fuel cell to facilitate the exchange of inactive enzymes with active enzymes, with the goal of extending the lifetime of laccase cathodes. Directly incorporating the laccase-producing fungus at the cathode extends the operational lifetime of laccase cathodes while eliminating the need for frequent replenishment of the electrolyte. The hybrid microbial–enzymatic cathode addresses the issue of enzyme inactivation by using the natural ability of fungi to exchange inactive laccases at the cathode with active laccases. Finally, enzyme adsorption was increased through the use of a functionally graded coating containing an optimized ratio of titanium dioxide nanoparticles and single-walled carbon nanotubes. The hybrid microbial–enzymatic fuel cell combines the higher current density of enzymatic fuel cells with the longevity of microbial fuel cells, and demonstrates the feasibility of a self-regenerating fuel cell in which inactive laccases are continuously exchanged with active laccases.

A Hybrid Microbial-Enzymatic Fuel Cell Cathode Overcomes Enzyme Inactivation Limits in Biological Fuel Cells

The construction of optimized biological fuel cells requires a cathode which combines the longevity of a microbial catalyst with the power density of an enzymatic catalyst. Laccase secreting fungi were grown directly on the cathode of a biological fuel cell to facilitate the exchange of inactive enzymes with active enzymes with the goal of extending the lifetime of laccase cathodes. Additionally, a functionally graded coating was developed to increase enzyme loading at the cathode. Directly incorporating the laccase producing fungus at the cathode extends the operational lifetime of laccase cathodes while eliminating the need for frequent replenishment of the electrolyte. Additionally, the hybrid microbial-enzymatic cathode addresses the issue of enzyme inactivation by using the natural ability of fungi to exchange inactive laccases at the cathode with active laccases. Finally, enzyme adsorption was increased through the use of a functionally graded coating containing an optimized ratio of titanium dioxide nanoparticles and single walled carbon nanotubes. The hybrid microbial-enzymatic fuel cell combines the higher power density of enzymatic fuel cells with the longevity of microbial fuel cells and demonstrates the feasibility of a self-regenerating fuel cell in which inactive laccases are continuously exchanged with active laccases.

Biological fuel cells produce bioelectricity with in-situ brackish water purification

Biological fuel cells, namely microbial desalination cells (MDCs) are a promising alternative to traditional desalination technologies, as microorganisms can convert the energy stored in wastewater directly into electricity and utilize it in situ to drive desalination, producing a high-quality reuse water. However, there are several challenges to be overcome in order to scale up from laboratory research. This study was conducted in order to better understand the performance of MDCs inoculated with marine sediments during the treatment of brackish water (5.0 g L−1 of NaCl) under three different configurations and cycles of desalination, envisaging the future treatment of saline wastewaters with conductivities lower than 10 mS cm−1. Results have shown that by increasing the desalination cycle three times, the efficiency of salt removal was improved by 3.4, 2.4 and 2.3 times for 1-MDC, 3-MDC, and 5-MDC, respectively. The same trend was observed for electrochemical data. Findings encourage further development of the MDC for sustainable brackish water and wastewater purification and future on-site utilization.

Pyrenyl-carbon nanostructures for scalable enzyme electrocatalysis and biological fuel cells

A large electrode geometric area-based pyrenyl carbon nanostructure modification for scale-up of electrocatalytic currents and power using hydrogenase anode and bilirubin oxidase cathode is demonstrated.