intercellular connection
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2021 ◽  
Vol 2021 ◽  
pp. 1-21
Author(s):  
Qiang Xie ◽  
Jun Zeng ◽  
Yongtao Zheng ◽  
Tianwen Li ◽  
Junwei Ren ◽  
...  

Background. Mitochondrial dysfunctions play a pivotal role in cerebral ischemia-reperfusion (I/R) injury. Although mitochondrial transplantation has been recently explored for the treatment of cerebral I/R injury, the underlying mechanisms and fate of transplanted mitochondria are still poorly understood. Methods. Mitochondrial morphology and function were assessed by fluorescent staining, electron microscopy, JC-1, PCR, mitochondrial stress testing, and metabolomics. Therapeutic effects of mitochondria were evaluated by cell viability, reactive oxygen species (ROS), and apoptosis levels in a cellular hypoxia-reoxygenation model. Rat middle cerebral artery occlusion model was applied to assess the mitochondrial therapy in vivo. Transcriptomics was performed to explore the underlying mechanisms. Mitochondrial fate tracking was implemented by a variety of fluorescent labeling methods. Results. Neuro-2a (N2a) cell-derived mitochondria had higher mitochondrial membrane potential, more active oxidative respiration capacity, and less mitochondrial DNA copy number. Exogenous mitochondrial transplantation increased cellular viability in an oxygen-dependent manner, decreased ROS and apoptosis levels, improved neurobehavioral deficits, and reduced infarct size. Transcriptomic data showed that the differential gene enrichment pathways are associated with metabolism, especially lipid metabolism. Mitochondrial tracking indicated specific parts of the exogenous mitochondria fused with the mitochondria of the host cell, and others were incorporated into lysosomes. This process occurred at the beginning of internalization and its efficiency is related to intercellular connection. Conclusions. Mitochondrial transplantation may attenuate cerebral I/R injury. The mechanism may be related to mitochondrial component separation, altering cellular metabolism, reducing ROS, and apoptosis in an oxygen-dependent manner. The way of isolated mitochondrial transfer into the cell may be related to intercellular connection.


Materials ◽  
2014 ◽  
Vol 7 (6) ◽  
pp. 4628-4639 ◽  
Author(s):  
Renchuan You ◽  
Xiufang Li ◽  
Yamei Xu ◽  
Yu Liu ◽  
Shenzhou Lu ◽  
...  

Cell Research ◽  
1993 ◽  
Vol 3 (2) ◽  
pp. 131-139
Author(s):  
Xiangyun Wei ◽  
Lingcheng Jian

1976 ◽  
Vol 39 (12) ◽  
pp. 1321-1328 ◽  
Author(s):  
SHUNKI YONEDA ◽  
KAZUHIKO TANAKA ◽  
HIROTAKA AKAGAMI ◽  
NOBUHIKO SHIBATA ◽  
SEIICHI TOYAMA

Science ◽  
1962 ◽  
Vol 137 (3531) ◽  
pp. 670-672
Author(s):  
Maynard M. Dewey ◽  
Lloyd Barr

Science ◽  
1962 ◽  
Vol 137 (3531) ◽  
pp. 670-672
Author(s):  
Maynard M. Dewey ◽  
Lloyd Barr

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