The Potency Of Actinomycetes From Deepsea Sediment Of Makassar Strait For Producing Antimicrobial Substances

A research on antimicrobial screening of 36 actinomycetes isolated from marine sediment of Makassar Strait through Widya Nusantara Expedition (EWIN) in 2013 and 2014, enzymatic characterization of potential isolates, morphological characterization and metabolite profiling of selected isolates has been conducted. The antimicrobial screening was conducted using paper disc, enzymatic characterization was using APIZYM Kit, morphological characterization was using Scanning Electron Microscope. And metabolite profiling was using TLC and GCMS. Five isolates retrieved from this research had ability to inhibit the growth of the four microbial testing, Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans, and the highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result showed that in GCMS, the active fraction was contained 4-amino-5-cyano-6-(4'methoxyphenyl)-1-methyl-2.3-dihydropyrrolo [2,3-B] pyridine which commercially used for bactericides and antihistamine.