MATHEMATICAL MODELING OF THE PROCESS OF FUMIGATION OF LEGUMINOUS SEEDS IN A DRUM-TYPE INSTALLATION

Intensification of post-harvest processing of leguminous crops is an urgent task for the enterprises of the agro-industrial complex. The improvement of the known methods of ensuring reliable pro-tection of leguminous crops from the effects of pathogenic microflora, quarantine insect pests is of particular interest for research. The most common method of disinfection of products is con-sidered fumigation - the elimination of insect pests and pathogens with a gas or vaporous toxic chemical. The article discusses the process of fumigation of leguminous crops by an ionized flow of an aerodisperse mixture in a drum-type installation, and also presents the results of mathemati-cal modeling of this process. The mathematical description of the proposed model of the fumiga-tion process was carried out using the Mathcad application package. The analytical study of the obtained mathematical model allowed us to establish a number of dependencies and patterns char-acterizing the course of the process of electrostatic fumigation of leguminous crops in the mode of constant mixing.

Leguminous Seeds Powder Diet Reduces the Survival and Development of the Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae)

Chemical storage pest control is interlinked with many challenges such as environmental pollution and toxicity to humans and animals. Alternative tools are thus being increasingly researched and applied to supplement and/or substitute old-fashioned chemical means. Entomotoxic proteins, such as the lectins of leguminous seeds, have been shown to be effective alternative control agents against many serious insect pests. The objective of this work was to evaluate the effect of the flour of three leguminous seeds, Phaseolus vulgaris L. (Fabaceae) (the common bean), Vicia faba L. (Fabaceae) (the broad bean) and Glycine max L. (Fabaceae) (the soya bean), against 4th instar larvae of Trogoderma granarium Everts (Coleoptera: Curculionidae). The flours were tested at different concentrations. They all demonstrated significant effects on larval mortality, as well as they all induced a decrease in the number of larvae reaching the pupal stage. The flours of P. vulgaris and V. faba were highly insecticidal against T. granarium larvae, especially at the highest concentrations (86.7% for PV100 and 90% for VF100). Our results enrich previous findings on the entomotoxic effect of leguminous plant lectins and highlight P. vulgaris and V. faba lectins as potential alternative control agents against T. granarium.

Acceptability and Satiety Evaluation of a Newly Developed Nutritionally Balanced School Meal

Five nutritious school meals were developed in a form of pastes based on leguminous seeds of Lentil, Chick pea, and Soya; and some dairy sources, all formulas contain groundnut, but few in crushed formed are used to test acceptance. The products were fortified with essential minerals and vitamins. Developed products were served as a breakfast meal to ~200 students (6-9 years juniors; 10-14 years seniors) in one of the Sudanese school where school feeding is in place (Omdurman City) for 21 days to substitute a traditional breakfast meal based on boiled Pigeon pea colloquially called “Balila” served routinely to the students. This paper covers acceptability of novel favorable products behavioral responses of senior and junior students to four meal/ subject interactions covering temptation to finishing the whole meal, reason(s) for not finishing the whole meal, time needed to finish the meal, and degree of satiety the students feel during class room hours.Almost 95% of the senior students interacted positively with pastes based on lentil or chick pea finishing or stop taking the meal in ? 10 minutes. All past products provide satiety; senior students judged on lentil based paste(s) as the meal that secures full satiety during school hours. Unlike seniors, more than 95% of junior students finished more of the soy based meal, one third of them took more time to finish lentil based meal, yet reflected satisfaction by taking less quantities from all products offered.. Degree of satiety feeling among junior students fluctuated where >90% of them showed satisfactory degree of satiety with all products offered.

Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations

Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk. (petai), and Archidendron jiringa (Jack) I.C. Nielsen (jengkol) seeds extracts contain sulfhydryl compounds and have various therapeutic properties. The main objective of this research was to compare the effect of various ethanol concentration on the yield of extract and levels of sulfhydryl compounds from leguminous seeds. Dried seeds were macerated at room temperature (25°C) with a ratio of solids and solvents was 1:10. The solvent concentrations used were 30%, 50%, 70% and 96% ethanol. The yield of extract was expressed as the ratio of the weight of extract to the weight of dried seeds, the level of sulfhydryl compound was expressed as reduced L-glutathione (GSH) equivalent. The level of sulfhydryl compounds was determined by Ellman reagent and further analyzed using a spectrophotometer at 411 nm wavelength. Stink bean produced the highest yield of extract, while the highest level of sulfhydryl compounds was obtained from petai cina seed extract. The concentration of ethanol gave a significant difference to the yield of extract and the level of sulfhydryl compounds. The results showed that ethanol concentration affected the efficiency of the extraction of total sulfhydryl compounds and the yield of the extract on leguminous seeds.

Studies on Effect of pH, Temperature & Light on Protease Production in Leguminous seeds.

Leguminous seeds are one of the important dilatory food of human being. It is the major cultivated food crop in Maharashtra and India. Ten species were isolated from ten different seeds of Legume, out of these Fusarium species only three Fusarium species were tested for the study of the effect of PH, Temperature and Light on Protease Production. The results show that the pH 5.5 and 6.5 is more favourable for Protease activity in F. moniliforme and F. oxysporium. Whereas, F. roseum shows poor activity for Protease Production. The Performance of Temperature on Protease Production was found to be very good at 20c in tested Fusarium species and for Light, the Continuous light showed strong support in F. moniliforme, F. oxysporum and F. roseum. The detail results are shown in the paper.

Recent advances in the development of legumain-selective chemical probes and peptide prodrugs

Legumain, which is also known as vacuolar processing enzyme (VPE) or asparaginyl endopeptidase (AEP), is a cysteine protease that was first discovered and characterized in the leguminous seeds of the moth bean in the early 1990s. Later, this enzyme was also detected in higher organisms, including eukaryotes. This pH-dependent protease displays the highest activity in acidic endolysosomal compartments; however, legumain also displays nuclear, cytosolic and extracellular activity when stabilized by other proteins or intramolecular complexes. Based on the results from over 25 years of research, this protease is involved in multiple cellular events, including protein degradation and antigen presentation. Moreover, when dysregulated, this protease contributes to the progression of several diseases, with cancer being the well-studied example. Research on legumain biology was undoubtedly facilitated by the use of small molecule chemical tools. Therefore, in this review, I present the historical perspectives and most current strategies for the development of small molecule substrates, inhibitors and activity-based probes for legumain. These tools are of paramount importance in elucidating the roles of legumain in multiple biological processes. Finally, as this enzyme appears to be a promising molecular target for anticancer therapies, the development of legumain-activated prodrugs is also described.