Genetic factors of functional traits

Selection of functional traits is a challenge for researchers, but an increasingly necessary objective due to the growing concern regarding animal welfare and overcoming the problems of reducing antibiotic use in rabbit production without undermining the animals’ productivity. The aim of this review is to discuss the genetic control of resistance to diseases, longevity and variability of birth weight within a litter, or litter size variability at birth within doe, describing the selection programmes and the first results from a multi-omics analysis of resistance/susceptibility to diseases. The heritability is around 0.13 for longevity, 0.01 for uniformity in birth weight, 0.09 for litter size variability and around 0.11 for disease resistance. Genetic correlations between functional traits and production traits are mostly no different from zero, or are moderately favourable in some cases. Six selection programmes developed in three countries are reviewed. Line foundation with high pressure for selection or divergent selection experiments are different methodologies used, and favourable responses to selection have been achieved. Genomics studies have revealed associations in regions related to immune system functionality and stress in lines selected for litter size variability. Knowledge of the role of gut microbiota in the rabbit’s immune response is very limited. A multi-omics approach can help determine the microbial mechanisms in regulation immunity genes of the host.

Global Characterization of Fungal Mitogenomes: New Insights on Genomic Diversity and Dynamism of Coding Genes and Accessory Elements

Fungi comprise a great diversity of species with distinct ecological functions and lifestyles. Similar to other eukaryotes, fungi rely on interactions with prokaryotes and one of the most important symbiotic events was the acquisition of mitochondria. Mitochondria are organelles found in eukaryotic cells whose main function is to generate energy through aerobic respiration. Mitogenomes (mtDNAs) are double-stranded circular or linear DNA from mitochondria that may contain core genes and accessory elements that can be replicated, transcribed, and independently translated from the nuclear genome. Despite their importance, investigative studies on the diversity of fungal mitogenomes are scarce. Herein, we have evaluated 788 curated fungal mitogenomes available at NCBI database to assess discrepancies and similarities among them and to better understand the mechanisms involved in fungal mtDNAs variability. From a total of 12 fungal phyla, four do not have any representative with available mitogenomes, which highlights the underrepresentation of some groups in the current available data. We selected representative and non-redundant mitogenomes based on the threshold of 90% similarity, eliminating 81 mtDNAs. Comparative analyses revealed considerable size variability of mtDNAs with a difference of up to 260 kb in length. Furthermore, variation in mitogenome length and genomic composition are generally related to the number and length of accessory elements (introns, HEGs, and uORFs). We identified an overall average of 8.0 (0–39) introns, 8.0 (0–100) HEGs, and 8.2 (0–102) uORFs per genome, with high variation among phyla. Even though the length of the core protein-coding genes is considerably conserved, approximately 36.3% of the mitogenomes evaluated have at least one of the 14 core coding genes absent. Also, our results revealed that there is not even a single gene shared among all mitogenomes. Other unusual genes in mitogenomes were also detected in many mitogenomes, such as dpo and rpo, and displayed diverse evolutionary histories. Altogether, the results presented in this study suggest that fungal mitogenomes are diverse, contain accessory elements and are absent of a conserved gene that can be used for the taxonomic classification of the Kingdom Fungi.

The evolution of body size in termites

Termites are social cockroaches. Because non-termite cockroaches are larger than basal termite lineages, which themselves include large termite species, it has been proposed that termites experienced a unidirectional body size reduction since they evolved eusociality. However, the validity of this hypothesis remains untested in a phylogenetic framework. Here, we reconstructed termite body size evolution using head width measurements of 1638 modern and fossil termite species. We found that the unidirectional body size reduction model was only supported by analyses excluding fossil species. Analyses including fossil species suggested that body size diversified along with speciation events and estimated that the size of the common ancestor of modern termites was comparable to that of modern species. Our analyses further revealed that body size variability among species, but not body size reduction, is associated with features attributed to advanced termite societies. Our results suggest that miniaturization took place at the origin of termites, while subsequent complexification of termite societies did not lead to further body size reduction.

Ontogeny, evolution and palaeogeographic distribution of the world’s largest ammonite Parapuzosia (P.) seppenradensis (Landois, 1895)

The world’s largest ammonite, Parapuzosia (P.) seppenradensis (Landois, 1895), fascinated the world ever since the discovery, in 1895, of a specimen of 1.74 metres (m) diameter near Seppenrade in Westfalia, Germany, but subsequent findings of the taxon are exceedingly rare and its systematic position remains enigmatic. Here we revise the historical specimens and document abundant new material from England and Mexico. Our study comprises 154 specimens of large (< 1 m diameter) to giant (> 1m diameter) Parapuzosia from the Santonian and lower Campanian, mostly with stratigraphic information. High-resolution integrated stratigraphy allows for precise cross-Atlantic correlation of the occurrences. Our specimens were analysed regarding morphometry, growth stages and stratigraphic occurrence wherever possible. Our analysis provides insight into the ontogeny of Parapuzosia (P.) seppenradensis and into the evolution of this species from its potential ancestor P. (P.) leptophylla Sharpe, 1857. The latter grew to shell diameters of about 1 m and was restricted to Europe in the early Santonian, but it reached the Gulf of Mexico during the late Santonian. P. (P.) seppenradensis first appears in the uppermost Santonian- earliest Campanian on both sides of the Atlantic. Initially, it also reached diameters of about 1 m, but gradual evolutionary increase in size is seen in the middle early Campanian to diameters of 1.5 to 1.8 m. P. (P.) seppenradensis is characterized by five ontogenetic growth stages and by size dimorphism. We therefore here include the many historic species names used in the past to describe the morphological and size variability of the taxon. The concentration of adult shells in small geographic areas and scarcity of Parapuzosia in nearby coeval outcrop regions may point to a monocyclic, possibly even semelparous reproduction strategy in this giant cephalopod. Its gigantism exceeds a general trend of size increase in late Cretaceous cephalopods. Whether the coeval increase in size of mosasaurs, the top predators in Cretaceous seas, caused ecological pressure on Parapuzosia towards larger diameters remains unclear.

Density and Abundance Estimation of Amazonian River Dolphins: Understanding Population Size Variability

The dolphins Inia geoffrensis—boto and Sotalia fluviatilis—tucuxi are threatened cetaceans inhabiting river ecosystems in South America; population numbers are still lacking for many areas. This paper provides density and abundance estimations of boto and tucuxi in 15 rivers sampled during the past nine years as part of a multinational research alliance. Visual boat-survey data collection protocols and analyses have been developed since 2012 (based on Distance Sampling methods) and recently reviewed (2019) to improve robustness and comparability. Differences across the sampled rivers and the analyzed river basins (Amazon and Orinoco) pointed to a density/population size gradient with lower densities and abundances observed in the Orinoco basin (0.9–1.5 ind./km²), passing through the eastern Amazon basin (2–5 ind./km²), and the largest numbers found at the central Brazilian Amazon (lower Purus River—2012 (14.5 boto/km², N = 7672; 17.1 tucuxi/km², N = 9238)). However, in other parts of the central Amazon, the density of dolphins was smaller than expected for high productive whitewater rivers (1–1.7 ind./km² in the Japurá and Solimões rivers). We attributed these differences to specific features of the basin (e.g., hydro-geomorphology) as well as to the cumulative effects of anthropogenic activities.

Residual Strength and Drying Behavior of Concrete Reinforced with Recycled Steel Fiber from Tires

Fiber reinforcement of concrete is an effective technique of providing ductility to concrete, increasing its flexural residual strength while reducing its potential for cracking due to drying shrinkage. There are currently a wide variety of industrial fibers on the market. Recycled steel fibers (RSF) from tires could offer a viable substitute of industrialized fibers in a more sustainable and eco-friendly way. However, mistrust exists among users, based on fear that the recycling process will reduce the performance, coupled with the difficulty of characterization of the geometry of the RSF, as a consequence of the size variability introduced by the recycling process. This work compares the behavior of RSF from tires compared with industrialized steel or polypropylene fibers, evaluating the fresh state, compressive strength, flexural residual strength, and drying behavior. The concept of Equivalent Fiber Length (EFL) is also defined to help the statistical geometrical characterization of the RSF. A microstructural analysis was carried out to evaluate the integration of the fiber in the matrix, as well as the possible presence of contaminants. The conclusion is reached that the addition of RSF has a similar effect to that of industrialized fibers on concrete’s properties when added at the same percentage.

Phenotypic (Fruit and Seed Characters) Selection of Dacryodes edulis (Don. G. Lam H. J.) Tree for Vegetative Propagation

The study was conducted to identify superior D. edulis trees using phenotypic characters (fruit and seed size) as the criteria to select candidate trees for subsequent multiplication through vegetative propagation. Five D. edulis compound trees were randomly selected within Onne community in Eleme LGA Rivers state in a preliminary effort to assist households in the selection and multiplication of desirable tree phenotypes. Seeds were extracted by softening fruit with warm water at 57oC. Fruit and seed length (mm), breadth (mm), and thickness (mm) were taken using veneer caliper. Size of fruit and seed was calculated as: length × breadth × thickness. The experiment was a completely randomized design in its layout and data analysis was carried out using analysis of variance and regression after a normality test was conducted using Shapiro-Wilk. The results showed that highest mean fruit size (79.38 ± 3.99 mm) was recorded in tree 3 and the lowest was tree 5 (29.60 ± 1.48 mm): while, highest seed size (34.78 ± 2.47 mm) was recorded in tree 3 and the lowest in tree 4 (15.58 ± 0.99 mm). Highest within tree fruit size variability was recorded in tree 1 (24%) and the lowest in tree 4 (12%): while the highest within tree seed size variability was recorded in tree 2 (28%) and the lowest in tree 5 (15%). There was however a significant difference in fruit and seed size between the trees. Pairwise comparison showed that tree 1 was not significantly different from tree 2 in fruit and seed size. There was a significant positive correlation between fruit and seed size among the trees. This implies that selection for large fruit size automatically selects for large seeds size. Large fruited trees can therefore be multiplied from these trees using vegetative propagation.

Benthic macroinvertebrates as reference indicators for monitoring of anthropogenic isotope 137Cs contamination in the marine environment

Due to the limited studies dealing with isotopes levels in benthic macroinvertebrates other than Mytilus sp. in period 2011–2018, macroinvertebrates and seawater samples from the southern Baltic Sea were collected. Activity of 137Cs was measured in most common benthic macroinvertebrates inhabiting southern Baltic Sea in the context of choosing the best bioindicators. Taking into account the narrow range of CR coefficients, statistically significant correlation between 137Cs activities in seawater and species, a high frequency of occurrence and thus high biomass and the common occurrence, only Limecola balthica could potentially serve as indicators for shallow and deep-sea areas accordingly. Widely spread Mytillus trossulus was characterised by large size variability in samples and shows no significant correlation between activities in species and seawater and appears not so common as L. balthica.