Mitochondrial DNA restriction site variation within and among five populations of Alaskan coho salmon (Oncorhynchus kisutch)

Mitochondrial DNA (mtDNA) from three Gulf of Alaska and two Bering Sea populations of coho salmon (Oncorhynchus kisutch) was assayed with 21 restriction endonucleases. A restriction site map was constructed for 15 enzymes that recognized hexanucleotide sequences and aligned to the rainbow trout (Oncorhynchus mykiss) gene map using the restriction map and known coho salmon sequences. The restriction site map and gene order of the coho salmon mtDNA genome are consistent with those of rainbow trout. Variation was observed for six enzymes at 10 sites that resulted in eight haplotypes. Variability and divergence observed exceeded those previously observed for allozyme loci in coho salmon. When variability that occurred only in single individuals was eliminated, two haplotypes that differed by four restriction sites remained. Variation at these sites was confirmed from polymerase chain reaction (PCR) amplified fragments. Bering Sea populations exhibited more variation than Gulf of Alaska populations. Explanations for the differences include more recent colonization by or smaller sizes of Gulf of Alaska populations.

Genome organization and restriction site map of the mitochondrial DNA of the housefly (Musca domestica)

A restriction map of the housefly mitochondrial DNA was constructed. The putative arrangement of the major coding regions was determined by two methods. In the first, Drosophila clones of known gene content were hybridized to housefly restriction fragments on Southern blots. In the second, rare restriction sites and clusters of restriction sites were used to align the housefly restriction map with that of Drosophila yakuba. Both methods produced the same apparent gene arrangement.Key words: mtDNA, restriction site map, Diptera, Muscidae, insect, evolution.