Methylation of f2rl3, cdkn2a genes and sudden cardiac death

The aim of the study was to evaluate the association of methylation of the F2RL3, CDKN2A gene with sudden cardiac death (SCD). Material and methods. Case-control study design. The SCD group included 150 deceased men (mean age 46.7 ± 9.2 years) with the main pathological diagnoses of acute circulatory failure, acute coronary insufficiency, which meets the SCD criteria of the European Society of Cardiology. The control group included 150 men who died suddenly, but not due to cardiovascular pathology (mean age 42.6 ± 1.2 years). DNA was isolated by phenol-chloroform extraction from myocardial tissue in both groups. The methylation status of the F2RL3 gene (19: 16890405-16890606, GRCh38.p13) and the CDKN2A gene (9: 21974726-21974877, GRCh38.p13) was assessed by methyl-specific polymerase chain reaction. Results. In the SCD group, 17.3 % (26/150) had the F2RL3 gene completely methylated (MM); in 6.0 % (9/150) it is completely unmethylated (UU); 76.7 % (115/150) had both methylated and unmethylated F2RL3 (MU) gene. In the control group, 16 % (24/150) had the F2RL3 gene completely methylated (MM); in 5.3 % (8/150), it is completely unmethylated (UU); 78.7 % (118/150) had both methylated and unmethylated F2RL3 (MU) gene. When comparing the groups, there were no statistically significant differences in the methylation status of the F2RL3 gene between the groups (p > 0.05). In all subjects in the SCD group and the control group, the CDKN2A gene is completely unmethylated. Conclusions. Methylation of genes F2RL3, CDKN2A is not associated with sudden cardiac death.

About brain disorders in acute coronary insufficiency

In acute coronary insufficiency, cerebral vascular disorders are not uncommon. NK Bogolepov calls the resulting states a combined coronary-cerebral crisis.

Evaluation of anthropometric results after acupuncture and diet applications in obesity patients:an experimental-control study

Obesity is a disease defined by excessive fat storage in the body. It is an energy balance problem; the increase in body fat is caused by an imbalance between energy intake and energy expenditure. This study aimed to determinate the changes in the anthropometric measurements of patients after acupuncture treatment used in obesity treatment. The present study was a cross-sectional study conducted in 2019 in xxx University's GETAT Center). Patients aged between 18–65 years with a BMI > 25 were included in the study. The participants were divided into two groups: Group 1 (acupuncture and diet) and Group 2 (diet only). Exclusion criteria: Pregnant women, breastfeeding patients , susceptible demographics (acute coronary insufficiency, immunodeficiency, severe psychotic disorder, liver and kidney failure), and those who could not give consent were not included in the study. Weight, BMI, and metabolic age values were measured with a Tanita device; the same diagnostic device was used for all patients. Both body and ear acupuncture were performed. In patients with all acupuncture, the yuan points of abnormal meridians were used for pulse diagnosis. ST 24, 25, and 36 (ZuSanLi); GV 20, 21, 5, 5, and LR 3 (Taichong); SP 6 and 9, GB 34, UB 62, HT 7, LU 9, and PC 6 (Nei Guan); and SI 3 and EXT-HN 3 (Yin-Tang) points were dewed. For ear acupuncture, hunger, kidney, larynx, stomach, jerome, and shen-men points were taken. A t-test was applied to pre- and post-acupuncture measurement values. After acupuncture, the waist circumference of the participants decreased significantly (p < 0.005). The BMI of the participants also decreased significantly after acupuncture (p < 0.005). In these studies, LU 6, ST 40, ST 21, K 4, ST 36 and 25, and LI 11 body points were used, while hunger, stomach, and shen-men points were commonly used as ear points.

Body Composition Rate Results after Acupuncture and Diet Applications in Women with Obesity Patients

Obesity is a disease defined by excessive fat storage in the body. It is an energy balance problem; the increase in body fat is caused by an imbalance between energy intake and energy expenditure. Method/participants: The present study was a cross-sectional study conducted in 2019 in xxx University’s GETAT Center). Patients aged between 18–65 years with a BMI > 25 were included in the study. Exclusion Criteria: Pregnant women, breastfeeding patients , susceptible demographics (acute coronary insufficiency, immunodeficiency, severe psychotic disorder, liver and kidney failure), and those who could not give consent were not included in the study. Weight, BMI, and metabolic age values were measured with a Tanita device; the same diagnostic device was used for all patients. Both body and ear acupuncture were performed. Results: A t-test was applied to pre- and post-acupuncture measurement values. After acupuncture, the waist circumference of the participants decreased significantly (p < 0.005). The BMI of the participants also decreased significantly after acupuncture (p < 0.005).

CLASSIFICATION OF MULTIDIMENSIONAL POLARIZATION MICROSCOPY RESULTS IN THE TECHNOLOGY OF FORENSIC INTELLECTUAL MONITORING OF HEART DISEASES

The work combines methods of multidimensional polarization microscopy, statistical processing of data and inductive modeling with the purpose of constructing a methodology for creation of intelligent systems for multi-level forensic medical monitoring based on the example of the post-mortem diagnosis of coronary heart disease and acute coronary insufficiency. The task of classifying the results of the study of biological materials for obtaining a diagnosis was solved. To obtain informative features, a model of biological tissue of the myocardium was developed and the main diagnostic parameters were determined (statistical moments of 1–4 orders of coordinate distributions of the values of azimuths and the ellipticity of polarization and their autocorrelation functions, as well as wavelet coefficients of the corresponding distributions), which are dynamic due to its necrotic changes. The classification of these data was provided by constructing a deciding rule in the multi – raw algorithm of the GMDH. The effectiveness of the described methodology has been experimentally proved.

Methods and means of asymutal-invariant muller matrix polyarimetry of optical and anisotropic biological layers

Objectives: The work is aimed at the theoretical substantiation and experimental development of the azimuthally invariant polarimetry method of partially depolarizing optical anisotropic biological layers on the basis of coordinate Muller-matrix mapping of histological sections for differential diagnostics of changes in optical anisotropy, which are associated with the emergence of pathological states. Results: The method of azimuthally invariant Muller-matrix mapping of optically anisotropic samples of the myocardium is proposed and grounded. The values of azimuthally invariant matrix element, superposition of matrix elements and the magnitude of the matrix vector distributions are obtained. Conclusion: The magnitude of the 1st-4th order statistical moments’ dependences, which characterize the distribution of the Muller-matrix invariant (MMI) of the histological sections of the myocardium are determined. The study of the possibility of differentiating causes of death due to ischemic heart disease (IHD) and acute coronary insufficiency (ACI) conducted from the standpoint of evidence-based medicine.

THE METHOD OF SPECTRALLY SELECTIVE LASER MUELLER MATRIX POLARIMETRY FOR VERIFICATION OF ACUTE CORONARY INSUFFICIENCY

INTRODUCTION: As the existing methods of evaluation of acute coronary insufficiency currently held is to some extent subjective. However, the specificity of forensic medicine requires objective methods. Thus, there is a necessity for objective methods of diagnosis for acute coronary insufficiency (ACI).OBJECTIVES: The objective of this study isto establish the diagnostic possibilities of the laser method, Muellermatrix polarimetry, on wavelength 450 nm of autofluorescence, with the method of statistical analysis of native heart slices to provide an after-death diagnosis of acute coronary insufficiency.METHODS: In our primary investigation we explored 69 human hearts with ACI, 69 with chronic ischemic heart disease, and 20 from corpses after violent deaths with short agonal period.The samples were studied with a Stokes polarimeter, which was modified to study laser induced autofluorescence.For comparison, histopathological studies were conducted by staining with hematoxylin, basic fuchsine, and picric acid. Data were analyzed statistically.RESULTS: Coordinate and probabilistic distributions of Mueller matrix invariant r14 and r41 laser polarization autofluorescence samples of myocardium were established. A visual determination of ACI, using coordinate distributions or probabilistic distributions, is difficult and hence, we conducted a statistical analysis between pixels of the image. For optically active fluorophores of the myocardium we found a growth (1.5 times) kurtosis of r41 value. Distribution of r41 decreased 1.6 times in the case of ACI.After definition of balanced accuracy, we noted definition of asymmetry and kurtosis of invariant r41with 89 % and 95% accuracy allowed a diagnosis of ACI.CONCLUSION: Using the spectrally selective laser method, Mueller-matrix polarimetry, on wavelength 450 nm of autofluorescence with method of statistical analysis of native heart slices allows for diagnosis of ACI with high accuracy.