Covalently Bonded Ir(IV) on Conducted Blue TiO2 for Efficient Electrocatalytic Oxygen Evolution Reaction in Acid Media

The stability of anode electrode has been a primary obstacle for the oxygen evolution reaction (OER) in acid media. We design Ir-oxygen of hydroxyl-rich blue TiO2 through covalent bonds (Ir–O2–2Ti) and investigate the outcome of favored exposure of different amounts of covalent Ir–oxygen linked to the conductive blue TiO2 in the acidic OER. The Ir-oxygen-blue TiO2 nanoclusters show a strong synergy in terms of improved conductivity and tiny amount usage of Ir by using blue TiO2 supporter, and enhanced stability using covalent Ir-oxygen-linking (i.e., Ir oxide) in acid media, leading to high acidic OER performance with a current density of 10 mA cm−2 at an overpotential of 342 mV, which is much higher than that of IrO2 at 438 mV in 0.1 M HClO4 electrolyte. Notably, the Ir–O2–2Ti has a great mass activity of 1.38 A/mgIr at an overpotential 350 mV, which is almost 27 times higher than the mass activity of IrO2 at the same overpotential. Therefore, our work provides some insight into non-costly, highly enhanced, and stable electrocatalysts for the OER in acid media.

Point of Care Ultrasound Use by Interns in Emergency Department

BACKGROUND: Point of care ultrasound (POCUS) is required for use in many instances on a daily basis in the hospital, especially in the emergency department and other specialty treatment areas. While interns play a crucial role in running emergency services in Thailand, the POCUS usage of interns has not been well studied. METHODS: This was a retrospective observational study of the interns who worked at the Department of Emergency Medicine, a tertiary university hospital in Khon Kaen, Thailand between July 2020 and April 2021. The seven questions survey about participants’ experiences performing POCUS examinations was conceived and carried out by the research team. The primary aim of this study is to identify the frequency of POCUS examinations performed by interns in this or any other given rotation. RESULTS: The response rate was 81.25% with the frequency of POCUS examinations per physician coming in at 2.5 per shift. It should be noted that examinations occurred primarily during the morning shift (79%) and were for diagnostic purposes (100%). The highest POCUS use cases (80%) were found to take place for abdominal examinations and the most commonly used POCUS application was the FAST (Focused Assessment with Sonography for Trauma) examination (92%). The confidence level was rated as 3/5. The primary barrier to performing POCUS was lack of knowledge or ultrasound training (47%). CONCLUSIONS: POCUS is often used by interns though less than in some other specialties. The type of POCUS application employed was limited due to lack of training and the primary obstacle for POCUS use in the emergency department was patient overcrowding.

Role and mechanism of miR-130a-3p in the chemosensitivity of retinoblastoma cells to vincristine

Purpose Chemoresistance remains the primary obstacle threatening the prognosis of retinoblastoma (RB). microRNAs (miRNAs) are acknowledged as critical regulator of drug resistance. This study explored the molecular mechanism of miR-130a-3p affecting the chemosensitivity of RB to vincristine (VCR). Methods miR-130a-3p expression of VCR-sensitive and VCR-resistant RB tissues was detected using RT-qPCR. VCR-resistant RB cell line Y79/VCR was induced. miR-130a-3p expression of Y79/VCR cell line and its corresponding parental cell line was detected. Y79/VCR cells were subjected to miR-130a-3p overexpression treatment. The cell proliferation was measured using MTT assay, and the IC50 value and drug resistance index were examined using CCK-8 assay. The targeting relationship between miR-130a-3p and PAX6 was predicted through bioinformatics analysis and verified using dual-luciferase assay. Functional rescue experiments were conducted to confirm the role of PAX6 in chemosensitivity of RB cells. The effect of miR-130a-3p on tumorigenesis and VCR sensitivity was observed in vivo. Results miR-130a-3p was downregulated in VCR-resistant RB tissues and cells. Overexpression of miR-130a-3p repressed the proliferation of VCR-resistant RB cells and enhanced chemosensitivity. miR-130a-3p targeted PAX6 expression. Overexpression of PAX6 reversed the effect of miR-130a-3p on chemosensitivity of RB. Overexpression of miR-130a-3p suppressed tumor growth and reduced VCR resistance in vivo. Conclusion miR-130a-3p enhanced the chemosensitivity of RB cells to VCR by targeting PAX6 expression.

Discovering cellular programs of intrinsic and extrinsic drivers of metabolic traits using LipocyteProfiler

A primary obstacle in translating genetics and genomics data into therapeutic strategies is elucidating the cellular programs affected by genetic variants and genes associated with human diseases. Broadly applicable high-throughput, unbiased assays offer a path to rapidly characterize gene and variant function and thus illuminate disease mechanisms. Here, we report LipocyteProfiler, an unbiased high-throughput, high-content microscopy assay that is amenable to large-scale morphological and cellular profiling of lipid-accumulating cell types. We apply LipocyteProfiler to adipocytes and hepatocytes and demonstrate its ability to survey diverse cellular mechanisms by generating rich context-, and process-specific morphological and cellular profiles. We then use LipocyteProfiler to identify known and novel cellular programs altered by polygenic risk of metabolic disease, including insulin resistance, waist-to-hip ratio and the polygenic contribution to lipodystrophy. LipocyteProfiler paves the way for large-scale forward and reverse phenotypic profiling in lipid-storing cells, and provides a framework for the unbiased identification of causal relationships between genetic variants and cellular programs relevant to human disease.

Targeting Immune Modulators in Glioma While Avoiding Autoimmune Conditions

Communication signals and signaling pathways are often studied in different physiological systems. However, it has become abundantly clear that the immune system is not self-regulated, but functions in close association with the nervous system. The neural–immune interface is complex; its balance determines cancer progression, as well as autoimmune disorders. Immunotherapy remains a promising approach in the context of glioblastoma multiforme (GBM). The primary obstacle to finding effective therapies is the potent immunosuppression induced by GBM. Anti-inflammatory cytokines, induction of regulatory T cells, and the expression of immune checkpoint molecules are the key mediators for immunosuppression in the tumor microenvironment. Immune checkpoint molecules are ligand–receptor pairs that exert inhibitory or stimulatory effects on immune responses. In the past decade, they have been extensively studied in preclinical and clinical trials in diseases such as cancer or autoimmune diseases in which the immune system has failed to maintain homeostasis. In this review, we will discuss promising immune-modulatory targets that are in the focus of current clinical research in glioblastoma, but are also in the precarious position of potentially becoming starting points for the development of autoimmune diseases like multiple sclerosis.

Enhancing Kidney Vasculature in Tissue Engineering—Current Trends and Approaches: A Review

Chronic kidney diseases are a leading cause of fatalities around the world. As the most sought-after organ for transplantation, the kidney is of immense importance in the field of tissue engineering. The primary obstacle to the development of clinically relevant tissue engineered kidneys is precise vascularization due to the organ’s large size and complexity. Current attempts at whole-kidney tissue engineering include the repopulation of decellularized kidney extracellular matrices or vascular corrosion casts, but these approaches do not eliminate the need for a donor organ. Stem cell-based approaches, such as kidney organoids vascularized in microphysiological systems, aim to construct a kidney without the need for organ donation. These organ-on-a-chip models show complex, functioning kidney structures, albeit at a small scale. Novel methodologies for developing engineered scaffolds will allow for improved differentiation of kidney stem cells and organoids into larger kidney grafts with clinical applications. While currently, kidney tissue engineering remains mostly limited to individual renal structures or small organoids, further developments in vascularization techniques, with technologies such as organoids in microfluidic systems, could potentially open doors for a large-scale growth of whole engineered kidneys for transplantation.

Vibration error compensation algorithm in the development of laser interference absolute gravimeters

Measurement error arising from vibration interference is recognized as the primary obstacle limiting the accuracy and stability of laser interference absolute gravimeters. The present work addresses this issue by proposing a global search optimization algorithm that determines the optimal absolute value of gravity based on the measured time–displacement coordinates of a falling body and the signal obtained from the passive vibration isolation system of the inertial reference corner cube in a laser interference absolute gravimeter. Results of numerical calculations conducted under vibration interference conditions with added white noise resulting in a signal-to-noise ratio of 40 dB demonstrate the following. The accuracy and standard deviation of the gravimeter obtained using the proposed algorithm are −0.04 µGal (1µGal=1×10-8 m s−2) and 0.24 µGal, respectively, while those values obtained by the standard least-squares solution are 10.19 and 154.11 µGal, respectively. The test results indicate that the average response of the reference value of acceleration due to gravity superimposed by a disturbance of 1.00 µGal is 1.01 µGal using the proposed algorithm and 0.87 µGal using the standard least-squares solution.

Veganism, Moral Motivation and False Consciousness

Despite the strength of arguments for veganism in the animal rights literature, alongside environmental and other anthropocentric concerns posed by industrialised animal agriculture, veganism remains only a minority standpoint. In this paper, I explore the moral motivational problem of veganism from the perspectives of moral psychology and political false consciousness. I argue that a novel interpretation of the post-Marxist notion of political false consciousness may help to make sense of the widespread refusal to shift towards veganism. Specifically, the notion of false consciousness fills some explanatory gaps left by the moral psychological notion of akrasia, often understood to refer to a weakness of will. Central to my approach is the idea that animal exploitation is largely systemic and the assumption that moral motivation is inseparable from moral thinking. In this light, the primary obstacle to the adoption of veganism arises not so much from a failure to put genuine beliefs into action, but rather in a shared, distorted way of thinking about animals. Thus, common unreflective objections to veganism may be said to be manifestations of false consciousness.

The Catholic Religious Presence in Civil Society: A Waning Influence

The Catholic Church is becoming a waning influence in global civil society. This is due, in part, to demographic changes that show an increasing loss of adherents within the Church’s traditional strongholds. Coupled with the growth of liberal social policies and continuing revelations about the crimes of sexual abuse by its clergy, the Church is being forced to reconsider how to continue as a moral advocate in civil society. It has sought to do this is by recalibrating its position in global church-state relations, moving toward a non-ideological or “third way” of politics, and seeking non-partisan solutions to social justice needs. However, even this shift has not been sufficient to address the erosion of the Church’s positive, political influence globally. For the Church to be successful in this goal, it will be necessary to totally re-set its social agenda as well as its religious priorities. Such tasks, however, will be difficult at best and almost impossible to accomplish where the primary obstacle for successful political efficacy and internally meaningful change is the Church’s own mismanagement of its two-millennia-old ecclesiastical structure.

Penyuluhan Makanan Bergizi Seimbang Melalui Pendekatan Metode Ular Tangga

The health issue is still a primary obstacle in Indonesia. All sectors need to pay attention to it; otherwise, the human resource level will be low. In line with this socialization is needed to encourage children especially, to start aware of consuming a healthy diet. In addition, this can broaden children’s horizons about what they better eat. The method in this activity is by using snake ladder game which is more attractive and fun for children. This method, in fact, can enhance children's knowledge. During the pre-test, the correct answers were 11,9 on average; while after the socialization the right answers were 14,5 on average. It shows that the sharing knowledge by snake ladder game is effectively helpful for children to increase their knowledge.