Evaluation of Anti-Inflammatory Property of Maranta Arundinacea using Protein Denaturation Assay - An In vitro Study

Introduction: Maranta arundinacea is a perennial plant which is seen in Mexico, central and South America. It was introduced and cultivated for its starch rich root which is known as arrowroot. It is gluten free and good for digestion. It also boasts metabolic rate and healthy sleep. Arrow root aid in lowering cholesterol which decreases the risk of heart diseases. It is a good substitute for breast milk because it’s loaded with magnesium, zinc and iron which promotes the growth and development of infants. Arrow root extract is previously known for its antibacterial and antifungal activity and also it gives relief from skin rashes, acne and skin sores. Aim: The aim of this study is to evaluate the anti-inflammatory property of Maranta arundinacea using protein denaturation assay. Materials and Methods: Maranta arundinacea (Arrowroot) was purchased commercially. Extract was prepared by adding 50 ml of distilled water and boiled at 50 degree Celsius and filtered. The extract was concentrated up to 10ml and the anti-inflammatory property was checked using protein denaturation assay and the readings were noted using a calorimeter. % of inhibition= ControlO.D-sampleO.D ∗100/ControlO.D. Results: It showed good anti inflammatory activity and thus it can be used as a potent alternative for anti inflammatory drugs. Conclusion: Maranta arundinacea extract showed good potential for anti-inflammatory activity. Thus in future more research can be done for formulations of eco-friendly treatment methods.

Free radical scavenging activity of Maranta arundinacea assisted selenium nanoparticles

Selenium nanoparticles have role in toxic waste removal and bioremediation. In a recent study, biosynthesis of many metal and metal oxide nanoparticles by different plant extracts was found to be stable even after wet heat sterilization process. In some studies, biosynthesis of silver, gold, and selenium nanoparticles using microorganisms and plant and parts. In this present study we have synthesized selenium nanoparticles using arrow root and analysed for its free radical scavenging activity. 20 mM of sodium selenite was prepared using 60 mL of distilled water and 40 mL of Arrowroot extract. It was kept in the shaker. Readings were taken for 3 days. Centrifugation was done at 8000 rpm. Pellet was collected and was used for antioxidants activity that is free radical scavenging activity. The antioxidant property of arrow root mediated selenium nanoparticles are comparatively higher than the standard antioxidant property. Calculations were done on the basis of reading that was obtained. This study proves that there is antioxidant activity that is free radical scavenging activity adopted by selenium nanoparticles extracted from arrow root.

Maranta arundinacea root assisted zinc oxide nanoparticles and its characterisation using TEM and UV-vis spectroscopy

Arrow root is a tropical root crop cultivated for its starchy rhizomes that have carbohydrate contents. Zinc oxide nanoparticles have emerged a promising potential in biomedicine, especially in the fields of anticancer, wound healing, free radical scavenging and antimicrobial including antibacterial, antifungal and antiviral research fields, which might be worried with their potent ability to trigger extra reactive oxygen species (ROS) production, launch zinc ions, and induce mobile apoptosis. 1 gram of Arrow root powder extract was mixed with 100 mL of water and filtered for nanoparticles synthesis. The zinc sulphate was used as a precursor for the zinc oxide nanoparticles synthesis and 30 mM was used. 50 mL of freshly prepared root extract and zinc sulphate solution was added together for the synthesis of Zinc oxide nanoparticles, which was then dried and characterisedcharacterized using transmission electron microscope (TEM) and during reaction it was characterized using UVvis spectroscopy method. A spherical shaped nanoparticle with plant extract in the background was observed. The peak at 300 nm confirm the nanoparticles synthesis using root extract. Based on our results we confirmed the capability of arrow root for the zinc oxide nanoparticles synthesis and it will be used for the various biomedical application in future.

Antibacterial activity of selenium nanoparticles synthesized using Maranta arundinaecea root extract

Maranta arundinaecea is otherwise known as arrowroot. It promotes weight loss, treats diahorrea and stimulates the immune system. As Arrowroot is rich in starch, it helps in good digestion by mild laxative in regulating the bowel movement. Also it reduces stomach pain and bloating. Selenium is a semi conductor so it is used in making electrical wires. It is used in potent chemotherapy. It is an anti-cancer agent due to its invasion of apoptotic pathway and cell cycle arrest. Usually the arrow root extract is available as powder. The powder is diluted and mixed into sodium selenite solution and this combination is observed for 3 days. After 3 days, it is centrifuged and collected in a pellet form. The lactobacillus and streptococcus mutants are used for culture of organism for 1 day. The extract is tested for for anti-bacterial activity. Result- It shows the sector where it can inhibit in Streptococcus mutans and lactobacillus. Maranta arundinaecea root mediated selenium nanoparticles shows very good antibacterial activity will used in many biomedical applications is future.

PENGARUH CARA PENGOLAHAN PATI GARUT (Maranta arundinacea) SEBAGAI BINDER DAN LAMA PENYIMPANAN TERHADAP KUALITAS FISIK PELLET AYAM BROILER (The Effect of Processing Method of Arrow Root Tuber (Maranta arundinacea) as Binder and Length of Storage Time on Physical Quality Pellet Feed For Chicken Broiler)

Abstrak Rancangan penelitian dilakukan RAL faktorial (4 x 3) 3 ulangan. Faktor A adalah Proses pengolahan binder terdiri dari : A0 = binder CMC 1 %. A1 = binder diseduh d air panas, A2= binder tercampur ransum dan disemprot air panas. A3 = binder dalam ransum, dan dipanaskan dalam autoclave. Jumlah binder pati garut yang digunakan adalah 12% . Faktor B adalah lama penyimpanan, yaitu: L0 = 15 hari, L2 = 30 hari dan L3 = 45 hari. Peubah yang diamati adalah parameter fisik pellet. Data yang diperoleh di analisis varian (Anava) dan Uji Duncan. (Steel and Torrie, 1989). Hasil penelitian adalah tidak terdapat interaksi antara jenis pengolahan dan lama penyimpanan terhadap terhadap densitas, berat jenis, kadar air, kerapatan tumpukkan dan kerapatan pemadatan tumpukkan, tetapi terdapat interaksi antara jenis pengolahan dan lama penyimpanan terhadap durabilitas pellet. Jenis pengolahan berpengaruh sangat nyata pada densitas dan durabilitas. lama penyimpanan berpengaruh sangat nyata terhadap kadar air dan berat jenis tapi jenis pengolahan dan lama penyimpanan tidak berpengaruh nyata pada kerapatan tumpukkan dan kerapatan pemadatan tumpukkan. Kata Kunci : Pati garut, binder, pengolahan, penyimpanan, pellet, broiler, Abstract The research design was done by factorial Completely Randomized Design (4 x 3) 3 replications. Factor A is binder processing process consisting of: A0 = 1% CMC binder. A1 = binder brewed in hot water, A2 = binder mixed ration and sprayed with hot water. A3 = binder in ration, and heated in autoclave. The amount of starch binder used is 12%. Factor B is the length of storage, ie: L0 = 15 days, L2 = 30 days and L3 = 45 days. The observed variable is the physical parameter of pellet. Data obtained in the analysis of variants (Anava) and Duncan Test. (Steel and Torrie, 1989). The result of this research is there is no interaction between processing type and length of storage to density, specific gravity, water content, pile density and pile compaction density, but there is interaction between processing type and duration of storage to pellet durability. The type of treatment has a very significant effect on the density and durability. duration of storage has a very significant effect on water content and specific gravity but the type of processing and storage time has no significant effect on pile density and density of compact pile. Keywords: garut starch, binder, processing, storage, pellet, broiler

SERABUT KELAPA SEBAGAI PRODUK BODY PROTECTOR YANG ERGONOMIS DENGAN METODE BIO-SIZING

Serat alam yang berasal dari serabut kelapa banyak tumbuh di daerah tropis (tropical plant) dan memiliki jenis dengan jumlah yang sangat banyak tetapi masih belum dimanfaatkan secara optimal, sehingga memiliki kesempatan untuk dikembangkan sebagai bahan produk. Dengan pemahaman (1) sifat fisik dan morfologinya; (2) karakteristik coir fiber (serabut kelapa) sebagai salah satu serat alam yang dapat digunakan sebagai bahan produk yang mampu memenuhi kebutuhan aplikasi produk teknik belum menunjukkan hasil yang memuaskan. Agar bahan dapat digunakan maka dibutukan perlakuan serat agar dihasilkan sifat fisik dan kekuatan yang lebih baik. Berdasarkan kondisi tersebut maka dibuat produk pelindung yaitu produk body protector yang berasal dari coir fiber, dimana serabut kelapa memiliki karakteristik fisik yaitu kekuatan tarik, elastisitas, biodegradable, dan insulasi yang baik terhadap suhu. Dengan karakteristik tersebut maka dapat dilakukan proses biotreatmen. Proses awal dilakukan treatmen bio-scoring kemudian dilakukan proses bio-sizing yang dapat meningkatkan kualitas fisik serabut kelapa. Proses treatmen bio sizing menggunakan bahan latek, arrow root dan resin dan menghasilkan serabut kelapa nonwoven sehinga dapat digunakan menjadi produk body protector. Untuk mehasilkan produk body protector yang ergonomis maka dilakukan pengukuran antropometri pada orang dewasa dengan didapat hasil pengukuran. Peningkatan kualitas fisik coir fiber dengan metode bio sizing setelah mengalami treatmen menjadi MC berkisar antara 13,38% sampai 17,2% sedangkan MR berkisar antara 16,79%, sampai 20,77%, kekuatan 3,2 kg/mm sehingga dapat dijadikan produk body protector memiliki kualitas dan tingkat kenyamanan yang baik dengan disain ergonomis pengukuran antropometri dengan dimensi produk yang dirancang sesuai anthropometri untuk lebar bahu adalah terkecil 41cm dan terbesar 46,5 cm, ukuran dimensi lebar dada terkecil 86 cm terbesar 101,5 cm dan ukuran dimensi panjang badan terkecil 61,6 cm terbesar 64,4 cm Kata Kunci : Coir Fiber, Bio-Treatment Sizing, Body Protector Ergonomis