Application of DNA barcoding confirms the female, male, larva and host of Bocchus scobiolae Nagy (Hymenoptera: Dryinidae)

Bocchus scobiolae Nagy (Hymenoptera: Dryinidae, Bocchinae) was reared in Szeged, Hungary. The female, male and immature larva were associated by mitochondrial COI sequences. B. scobiolae, previously known only from Romania, is recorded for the first time from Hungary, Moldova, and Turkey. Caliscelis wallengreni (Stål) (Hemiptera: Caliscelidae) is mentioned for the first time as host of B. scobiolae. B. vernieri Olmi is indicated for the first time from Hungary. Helegonatopus rasnitzyni (Trjapitzin, 1963) (Hymenoptera: Encyrtidae), recorded for the first time from Hungary, was reared from B. scobiolae (new record).

Redescription of the female, male, larva and pupa of Sabethes (Sabethoides) glaucodaemon (Dyar & Shannon) (Diptera: Culicidae) and description of the female genitalia

Sabethes (Sabethoides) glaucodaemon was described for the first time by Dyar & Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa. (Sbo.) tridentatus are discussed.

Taenionema (Plecoptera: Taeniopterygidae), a stonefly genus new to the fauna of China

The taeniopterygid genus Taenionema Banks, 1905 is reported from China for the first time. A newly discovered and probable new species of this genus, Taenionema sp., is described and illustrated based on larvae from Zhejiang Province of coastal southeastern China. The species is characterized by the male larva having a bilobed post-genital plate. Adults will be necessary for a future formal species description. This contribution represents the first larval description in the family Taeniopterygidae from China.

New subgenus Monilistylus subgen. n. and a new species Procloeon (Monilistylus) ornatipennis sp. n. (Ephemeroptera: Baetidae: Procloeon)

The new subgenus Monilistylus subgen. n. is described based on imaginal and larval characters. Its type species is Procloeon (Monilistylus) monilistylus Kluge, Tiunova & Novikova 2014 from the Russian Far East, known as larvae, subimagines, imagines of both sexes and eggs associated by rearing. Male larva, subimago and imago of Procloeon (Monilistylus) ornatipennis sp. n. are described based on reared specimens from Lombok (Indonesia).

Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis

Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542).

Description of the adult male, larva and pupa of Topomyia (Topomyia) aureoventer (Diptera: Culicidae) in India

The original description of Topomyia aureoventer by Theobald (1910) included only the adult female. Description of the adult male, pupa and larva, with figures of the male genitalia, pupa and larva, are provided. The larva is predacious with well-developed mandibles and inhabits leaf axils of Colocasia plants.

A new species of Simulium (Simulium) (Diptera: Simuliidae), with keys to S. striatum species-group from India

Simulium (Simulium) yanaense sp. nov. is described based on the male, larva, and pupa. It is characterized by having a maxillary palp with a small sensory vesicle, and the subcosta and basal portion of the radius bare in the male; the gill with 10 short slender filaments and short common basal stalk in the pupa; and the thorax and abdomen without a pair of dorsolateral protuberances in the larva. Keys are provided to the species of the S. striatum species group of the subgenus Simulium known from India.

A new species of Liriomyza Mik (Diptera, Agromyzidae) on okra in Brazil

Liriomyza okrae sp. n. is a new species from Brazil that does not have the yellow thoracic spots usually characteristic of the genus. The specimens were collected in Rondônia state (Brazil), reared from okra—Abelmoschus esculentus (L.) Moench (Malvaceae). Detailed descriptions of adult male, larva and puparium are made, and images of the male, male terminalia, larva, puparium and host-plant are given. A key is provided for Neotropical Liriomyza with an entirely dark thorax.

A new Notacanthurus Tshernova, 1974 and a new Rhithrogena Eaton, 1881 (subgenus Tumungula Zhou & Peters, 2004) from Thailand (Heptageniidae, Ephemeroptera)

Notacanthurus baei sp. n. (larva) and Rhithrogena (Tumungula) siamensis sp. n. (male, female and assumed larva) are described from northern Thailand. Diagnoses and line drawings of key characters are provided. Larvae of Southeast Asian Notacanthurus n.sp. resemble those of Palearctic Notacanthurus spp. in having a median ridge of spines directed posteriorly on terga, a similar patterning of terga with oblique stripes sloping inward, femoral cross bands and caudal filaments with whorls of small spines. Contrastingly, larvae of the new species bear claws with denticles, while the mature male larva exhibits prospective penis with distally paired portions of rounded lobes like those in most Electrogena Zurwerra and Tomka, 1984. Imagines of R. (T.) siamensis represent a second species of subgen. Tumungula Zhou and Peters, 2004. Males have balloon-shaped hypertrophied foreclaws, with first tarsal segment about 1.4x the length of the second, and divergent penis lobes without titillators. In contrast to R. (T.) unica the male styliger plate shows two sharp, inside directed projections, submedian lobes of the penis have circular gonopores lacking subapical spines.