Molasses as a new nutrition medium for Scenedsmus quadricauda growth and production of some bio compounds

Algae comprise a large group of Thallophyta, which may be used as direct nutrition of human beings. Molasses is the by-product of the sugar manufacturing facility. In this study, a locally isolated Scendsmus quadricauda from the environment of Mosul in the Shalalat region was obtained. Biomass of Scenedsmus was measurement by carried out and filtration then drying in an oven for 24 h and weighed, Estimation of chlorophyll and protein and carbohydrate content of Scenedsmus. The research has proved that the best growing period for Scendsmue quadricauda is 15 days when using sugar factory waste as a carbon source, the growth reached (1.42 nm) as optical density, biomass (1525 mg /L), chlorophyll (green), pigment (18 mg /l) protein content (396 mg /l ) and carbohydrates ( 501 mg / l ). The research showed that the use of sugar factory waste as a nutritional medium for algal growth in the dark (11.5%) achieved good growth of Scendesmues quadricauda ( 0.632 nm), biomass (820 mg / L), green pigment (Chlorophyll) (18 mg /L) protein content (235 mg / L ) and carbohydrates (401 mg/L). while using phosphor (0.018%) of K2HPO4 in dark medium achieved highest growth rate (0.91 nm) , biomass (1110 mg / L) chlorophyll ( 22 mg/L) protein (301mg/L) and carbohydrate (461 mg/L) . It is noted too , that using IAA (0.5 g/L) in dark medium support best growth (0.888 nm) , biomass (1010 mg/L) chlorophyll (25 mg/L) , protein (230mg/L) and carbohydrate (440 mg//L) . The study showed that thiamine (1 g/L) in dark medium achieved highest growth (0.750 nm) biomass (218 mg/L), chlorophyll (29mg/L), protein (220 mg/L), carbohydrate (340mg/L). Therefore, using Molasses can enhance the growth, biomass, chlorophyll, protein, and carbohydrate content in the S. quadricauda.

Congenital chylous as cites in a 3-month-old infant in Zaria: A case report

Congenital chylous ascites (CCA) is a rare disease that results from the maldevelopment of the intra-abdominal lymphatic system. Due to the rarity of congenital chylous ascites and the lack of standards in diagnosis and therapy, this disease constitutes a medical challenge and individual therapy seems to be extremely important. A 3-month-old girl diagnosed with congenital chylous ascites. She was managed initially with nil per oral, parenteral nutrition, medium chain triglyceride (MCT) containing oil and abdominal paracentesis, followed by octreotide. Medium chain triglyceride formula, the main stay of management was discontinued with gradual reintroduction of breast feeds. This case was selected due to the rarity of CCA and the lack of standards in the diagnosis and therapy.

Hydroponic Garlic Production: An Overview

Introduction: Garlic is an important crop growing all around the globe for its nutritional and medicinal purpose. Due to climate change soil production of garlic is affected and thus lower in yield. For improving the yield and increasing the quality of vegetables a new technique was developed is called hydroponic technology. Review results: In hydroponic, garlic can be produced on condition basis by providing proper nutrition and growth environment including air, light, soilless mediums, and water requirements. Methods like deep water culture, nutrient film technique, drip irrigation, bubble ponics are very common under different soilless mediums which includes organic, inorganic and synthetic. A detailed overview on production technology, nutrition, medium and hydroponic techniques which are discussed in details are given below in this review.

Effects of Medium Components on Isocitric Acid Production by Yarrowia lipolytica Yeast

The microbiological production of isocitric acid (ICA) is more preferable for its application in medicine and food, because the resulting product contains only the natural isomer—threo-DS. The aim of the present work was to study ICA production by yeast using sunflower oil as carbon source. 30 taxonomically different yeast strains were assessed for their capability for ICA production, and Y. lipolytica VKM Y-2373 was selected as a promising producer. It was found that ICA production required: the limitation of Y. lipolytica growth by nitrogen, phosphorus, sulfur or magnesium, and an addition of iron, activating aconitate hydratase, a key enzyme of isocitrate synthesis. Another regulatory approach capable to shift acid formation to a predominant ICA synthesis is the use of inhibitors (itaconic and oxalic acids), which blocks the conversion of isocitrate at the level of isocitrate lyase. It is recommended to cultivate Y. lipolytica VKM Y-2373 under nitrogen deficiency conditions with addition of 1.5 mg/L iron and 30 mM itaconic acid. Such optimized nutrition medium provides 70.6 g/L ICA with a ratio between ICA and citric acid (CA) equal 4:1, a mass yield (YICA) of 1.25 g/g and volume productivity (QICA) of 1.19 g/L·h.

Factors affecting Miscanthus × giganteus adventitious shoots formation in vitro

The research was carried out at the Laboratory of Agrobiotechnology of the Institute of Biology and Plant Biotechnology, Faculty of Agronomy of Vytautas Magnus University (till 2019 Aleksandras Stulginskis University). The effect of basal nutrition medium, subculture and growth regulators on the adventitious shoots formation from isolated explants of Miscanthus × giganteus was analysed. The apical meristem of in vitro developed adventitious shoots of Miscanthus × giganteus was cultivated in the Woody Plant Medium (WPM) and Murashige and Skoog (MS) nutrition medium supplemented with different 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) combinations. It has been determined that the formation frequency of adventitious shoots on the WPM nutrition medium was from 5.0 to 18.5% (after the first subculture) and from 19.5 to 38.3% (after the second subculture) higher in comparison with the MS nutrition medium supplemented with the same combinations of growth regulators. It has been established that on the WPM nutrition medium the formation frequency of adventitious shoots after the second subculture was from 13.3 to 18.5% higher in comparison with the first subculture, while on the MS nutrition medium the effect of subculture was not significant. The most intensive frequency of shoots formation (48.8%) and the highest shoot number per explant (24.1 units) have been obtained in the WPM medium supplemented with 3.0 mg l–1 BAP + 0.4 mg l–1 IBA + 0.01 mg l–1 IAA after the second subculture.

Bamboo scraps and hardwood chips biochar derived from a cone-truncated open fire kiln for use as a nutrition medium for agricultural purpose

An experiment on the production of biochar from bamboo scraps and hardwood chips for use as materials to improve soil was done by burning the biomass of the bamboo scraps and hardwood chips in Pyrolysis conditions at a temperature range of 500 - 700C with a cone-truncated open fire kiln which reduces burning time to 45-60 min. Experimental results revealed that the average bamboo-scrap biochar of 2.5 kg resulted from burning 15 kg of the bamboo scraps; likewise, the average hardwood-chip biochar of 2.2 kg resulted from burning 15 kg of the hardwood chips. According to analyses, the bamboo-scrap biochar was composed of 8.6% moisture content, 8.0% volatile substance, 9.0% ash, 83% fixed carbon, 250mg/g iodine and pH 10.3; whereas, the hardwood-chip biochar was composed of 6% moisture content, 8.2% volatile substance, 3% ash , 88.8% fixed carbon, 300 mg/g iodine, and pH 10.1. The result of planting experiments showed that after mixing the biochar for soil nourishment and compost into the planting soil, the morning glory and kale can grow better than those planted in normal soil and soil with compost.

Medium-Dependent Antibacterial Properties and Bacterial Filtration Ability of Reduced Graphene Oxide

Toxicity of reduced graphene oxide (rGO) has been a topic of multiple studies and was shown to depend on a variety of characteristics of rGO and biological objects of interest. In this paper, we demonstrate that when studying the same dispersions of rGO and fluorescent Escherichia coli (E. coli) bacteria, the outcome of nanotoxicity experiments also depends on the type of culture medium. We show that rGO inhibits the growth of bacteria in a nutrition medium but shows little effect on the behavior of E. coli in a physiological saline solution. The observed effects of rGO on E. coli in different media could be at least partially rationalized through the adsorption of bacteria and nutrients on the dispersed rGO sheets, which is likely mediated via hydrogen bonding. We also found that the interaction between rGO and E. coli is medium-dependent, and in physiological saline solutions they form stable flocculate structures that were not observed in nutrition media. Furthermore, the aggregation of rGO and E. coli in saline media was observed regardless of whether the bacteria were alive or dead. Filtration of the aggregate suspensions led to nearly complete removal of bacteria from filtered liquids, which highlights the potential of rGO for the filtration and separation of biological contaminants, regardless of whether they include live or dead microorganisms.

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain virus-free planting material of Prunus domestica L. by chemotherapy in vitro. Ribavirin at concentrations of 10 to 50 mg·l−1 was added to Murashige–Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l−1 ribavirin was entirely ineffective for ACLSV and 10 to 30 mg·l−1 was ineffective for PNRSV elimination. Ribavirin concentrations of 40 and 50 mg·l−1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l−1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in ‘Magna Glauca’ at concentrations of 40 mg·l−1 was damaged, as the presence of polymorphic AFLP markers were observed.

Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica

Isocitric acid exists in the form of four stereoisomers, of which only the threo-Ds-form (ICA) is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use. In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica. The effects of temperature, pH of the medium, and aeration on the growth of the producer Y. lipolytica VKM Y-2373 and synthesis of ICA were studied. An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined. The producer is advised to carry out cultivation at 29°C and various pH of the medium and the oxygen concentration (pH 5 and pO2 20–25% (of saturation) during the growth period and pH 6 and pO2 50–55% (of saturation) during the acid formation) on a nutrient medium containing an increased content of zinc (0.6 mg/L), iron (1.2 mg/L), and 30 mM itaconic acid (inhibitor of isocitrate lyase—the key enzyme of ICA metabolism) should also be introduced into the nutrition medium. Such fermentation production mode provides 90.5 g/L ICA with process selectivity of 80%, mass yield (YICA) of 0.77 g/g, and energy yield (ηICA) of 0.278 g/g.

Effect of Agrobacterium rhizogenes on the development and alkaloid biosynthesis of Catharanthus roseus growing in an aeroponic system

The aim of this study is to improve the biomass and the content of secondary metabolites of the Catharanthus roseus using an aeroponic system with the addition of Agrobacterium rhizogenes. The results showed that the growth of Catharanthus roseus on the aeroponic system with the addition of A. rhizogennes into the nutrition medium at the concentration of 109 CFU/mL was better than the treatments without addition of A. rhizogennes. The leaf samples of plants having bud and fruits were extracted and analysed the content of vincristine and vinblastine by HPLC system. Higher content of vincristine and vinblastine was obtained from the treatment with addition of A. rhizogenes