acid succinate
Recently Published Documents


TOTAL DOCUMENTS

25
(FIVE YEARS 0)

H-INDEX

5
(FIVE YEARS 0)

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Laszlo Pecze ◽  
Elisa B. Randi ◽  
Csaba Szabo

Abstract Clinical observations and preclinical studies both suggest that Down syndrome (DS) may be associated with significant metabolic and bioenergetic alterations. However, the relevant scientific literature has not yet been systematically reviewed. The aim of the current study was to conduct a meta-analysis of metabolites involved in bioenergetics pathways in DS to conclusively determine the difference between DS and control subjects. We discuss these findings and their potential relevance in the context of pathogenesis and experimental therapy of DS. Articles published before July 1, 2020, were identified by using the search terms “Down syndrome” and “metabolite name” or “trisomy 21” and “metabolite name”. Moreover, DS-related metabolomics studies and bioenergetics literature were also reviewed. 41 published reports and associated databases were identified, from which the descriptive information and the relevant metabolomic parameters were extracted and analyzed. Mixed effect model revealed the following changes in DS: significantly decreased ATP, CoQ10, homocysteine, serine, arginine and tyrosine; slightly decreased ADP; significantly increased uric acid, succinate, lactate and cysteine; slightly increased phosphate, pyruvate and citrate. However, the concentrations of AMP, 2,3-diphosphoglycerate, glucose, and glutamine were comparable in the DS vs. control populations. We conclude that cells of subjects with DS are in a pseudo-hypoxic state: the cellular metabolic and bio-energetic mechanisms exhibit pathophysiological alterations that resemble the cellular responses associated with hypoxia, even though the supply of the cells with oxygen is not disrupted. This fundamental alteration may be, at least in part, responsible for a variety of functional deficits associated with DS, including reduced exercise difference, impaired neurocognitive status and neurodegeneration.


2019 ◽  
Author(s):  
Stavria Panayidou ◽  
Kaliopi Georgiades ◽  
Theodoulakis Christofi ◽  
Stella Tamana ◽  
Vasilis Promponas ◽  
...  

AbstractBacterial virulence may rely on secondary metabolism, but core metabolism genes are assumed to be necessary primarily for bacterial growth. To assess this assumption, we correlated the genome, the transcriptome and the pathogenicity of 30 fully sequenced Pseudomonas strains using two Drosophila and one mouse infection assay. In accordance with previous studies gene presence-absence does not explain differences in virulence among P. aeruginosa strains, but merely between P. aeruginosa and other Pseudomonas species. Similarly, classical gene expression analysis of highly vs. lowly pathogenic P. aeruginosa strains identifies many virulence factors, and only a few metabolism genes related to virulence. Nevertheless, assessing the virulence of 553 core metabolic and 95 random non-metabolic gene mutants of P. aeruginosa PA14, we found 16.5% of the core metabolic and 8.5% of the non-metabolic genes to be necessary for full virulence. Strikingly, 11.8% of the core metabolism genes exhibit defects in virulence that cannot be attributed to auxotrophy. The compromised in virulence metabolic gene mutants were mapped in multiple pathways and exhibited further defects in acute virulence phenotypes and in a mouse lung infection model. Functional transcriptomics re-analysis of core metabolism at the pathway level, reveals amino-acid, succinate, citramalate, and chorismate biosynthesis and beta-oxidation as important for full virulence and expression of these pathways indicative of virulence in various strains. Thus, P. aeruginosa virulence variation, which to this point remains unpredictably combinatorial at the gene level, can be dissected at the pathway level via combinatorial trancriptome and functional core metabolism analysis.


2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Gulsah Gundogdu ◽  
Fatma Demirkaya Miloglu ◽  
Onur Senol ◽  
Yavuzer Koza ◽  
Fuat Gundogdu

Abstract Acute coronary syndrome (ACS) is a clinical condition caused by a disturbance in myocardial blood flow. ACS can be basically divided into two forms: ST elevation myocardial infarction (STEMI) due to complete occlusion of the coronary artery and non-ST elevation myocardial infarction (NSTEMI) due to partial occlusion of the coronary artery. In this study, we aimed to monitor the metabolite profile of STEMI and NSTEMI patients and compare the results via untargeted metabolomics approach. Serum samples were collected from STEMI and NSTEMI patients, and each group consists of 20 participants. Extraction was achieved by acetonitrile, and chromatographic separation was performed by LC/Q-TOF/MS/MS accompanied with dual AJS ESI positive ion mode. METLIN, MATLAB 2017a-PLS Toolbox7.2, and Human Metabolome Database were utilized for bioinformatics evaluation of obtained findings. In our results, 203 m/z ratio was detected and 163 m/z ratio passed the significance criteria (fold analysis > 1.5 and p < 0.05). Twenty-five metabolites including BCAAs, LysoPC species, lactic acid, succinate, malonic acid, maleic acid, butyric acid, carnitine, and betaine were identified. In conclusion, new biomarker candidates were identified to differentiate the diagnosis of STEMI and NSTEMI. Identified metabolites are indicative of alterations in oxidative stress, hypoxia, TCA cycle, and amino acid metabolism.


2019 ◽  
Vol 2 (3) ◽  
pp. 317-343 ◽  
Author(s):  
Yao Zhao ◽  
Jing Bai ◽  
Qian Luo ◽  
Jing-Ying Zhang ◽  
Jia-Rui Xu ◽  
...  

Intrinsic drug resistance has been demonstrated in different types of breast cancer cells, leading to the recurrence of disease after treatment. Here, we report a functional drug liposome that enables electric charge conversion in the weak acidic milieu of cancer to enhance the treatment efficacy of different breast cancers. The functional drug liposomes were developed by encapsulating daunorubicin and rofecoxib, and modified with new functional material, D-alpha tocopherol acid succinate-polyethylene glycol-glutarate (TPGS1000-glutarate). The results demonstrated that the liposomes promoted the effects of cellular uptake and lysosomal escape, followed by targeting the mitochondria. Consequently, the electric charge conversable drug liposomes significantly enhanced the treatment efficacy by initiating a cascade of reactions through inducing autophagy and apoptosis in different breast cancer cells. In conclusion, the electric charge conversable drug liposomes enable to enhance treatment efficacy of different breast cancers, and hence the study could offer a broadly applicable strategy to enhance efficacy against heterogeneous and refractory cancer cells.


2014 ◽  
Vol 58 (6) ◽  
pp. 3411-3420 ◽  
Author(s):  
Luca Laghi ◽  
Gianfranco Picone ◽  
Federica Cruciani ◽  
Patrizia Brigidi ◽  
Fiorella Calanni ◽  
...  

ABSTRACTBacterial vaginosis (BV) is a common vaginal disorder characterized by the decrease of lactobacilli and overgrowth ofGardnerella vaginalisand resident anaerobic vaginal bacteria. In the present work, the effects of rifaximin vaginal tablets on vaginal microbiota and metabolome of women affected by BV were investigated by combining quantitative PCR and a metabolomic approach based on1H nuclear magnetic resonance. To highlight the general trends of the bacterial communities and metabolomic profiles in response to the antibiotic/placebo therapy, a multivariate statistical strategy was set up based on the trajectories traced by vaginal samples in a principal component analysis space. Our data demonstrated the efficacy of rifaximin in restoring a health-like condition in terms of both bacterial communities and metabolomic features. In particular, rifaximin treatment was significantly associated with an increase in the lactobacillus/BV-related bacteria ratio, as well as with an increase in lactic acid concentration and a decrease of a pool of metabolites typically produced by BV-related bacteria (acetic acid, succinate, short-chain fatty acids, and biogenic amines). Among the tested dosages of rifaximin (100 and 25 mg for 5 days and 100 mg for 2 days), 25 mg for 5 days was found to be the most effective.


Author(s):  
І. О. Костюк

У зв’язку із застосуванням бурштинової кислоти(сукцинату) як фактора метаболічної корекції в ра-ціонах тварин та у ветеринарній медицині, актуаль-ним є дослідження особливостей використання цієїречовини як енерґетичного субстрату тканинногодихання. Важливим для ефективного застосуванняметаболічної корекції є вивчення впливу вітаміну Е –як мембранотропного фактора – на окиснення сук-цинату мітохондріями печінки. Встановлено стиму-ляцію вільного окиснення сукцинату в мітохондріяхпечінки під дією високих доз вітаміну Е у раціоні ку-рей, що важливо враховувати в разі можливого сумі-сного застосування вітаміну Е і сукцинату, як енер-ґотропних факторів метаболічної корекції у птиці. The study of the peculiarities of the use of succinate as an energetic substrate of the tissue respiration is actual in connection with the use of the above acid (succinate) in the ration of animals and in veterinary medicine as one of the factors of metabolic correction. The investigation of the effect of membranotropic substances, namely vitamin E, on the oxidation of succinate by liver metochondria is important for the effective use of metabolic correction. The stimulation of free oxidation of succinate in liver mitochondria under the influence of high doses of vitamin E in the ration of hens has been shown. It should be taken into consideration when both vitamin E and succinate are used as energotrope factors of metabolic correction in poultry.


2009 ◽  
Vol 70 (3-4) ◽  
pp. 551-555 ◽  
Author(s):  
Jingyuan Wang ◽  
Baogen Su ◽  
Yun Su ◽  
Yiwen Yang ◽  
Qilong Ren

Sign in / Sign up

Export Citation Format

Share Document