Pan-ROCK and ROCK2 Inhibitors Affect Dexamethasone-Treated 2D- and 3D-Cultured Human Trabecular Meshwork (HTM) Cells in Opposite Manners

Effects of a pan-ROCK-inhibitor, ripasudil (Rip), and a ROCK2 inhibitor, KD025 on dexamethasone (DEX)-treated human trabecular meshwork (HTM) cells as a model of steroid-induced glaucoma were investigated. In the presence of Rip or KD025, DEX-treated HTM cells were subjected to permeability analysis of 2D monolayer by transendothelial electrical resistance (TEER) and FITC–dextran permeability, physical properties, size and stiffness analysis (3D), and qPCR of extracellular matrix (ECM), and their modulators. DEX resulted in a significant increase in the permeability, as well as a large and stiff 3D spheroid, and those effects were inhibited by Rip. In contrast, KD025 exerted opposite effects on the physical properties (down-sizing and softening). Furthermore, DEX induced several changes of gene expressions of ECM and their modulators were also modulated differently by Rip and KD025. The present findings indicate that Rip and KD025 induced opposite effects toward 2D and 3D cell cultures of DEX-treated HTM cells.

Stiffness Analysis to Predict the Spread Out of Fake Information

This work highlights how the stiffness index, which is often used as a measure of stiffness for differential problems, can be employed to model the spread of fake news. In particular, we show that the higher the stiffness index is, the more rapid the transit of fake news in a given population. The illustration of our idea is presented through the stiffness analysis of the classical SIR model, commonly used to model the spread of epidemics in a given population. Numerical experiments, performed on real data, support the effectiveness of the approach.

An Efficient Polynomial Chaos Method for Stiffness Analysis of Air Spring Considering Uncertainties

Traditional methods for stiffness analysis of the air spring are based on deterministic assumption that the parameters are fixed. However, uncertainties have widely existed, and the mechanic property of the air spring is very sensitive to these uncertainties. To model the uncertainties in the air spring, the interval/random variables models are introduced. For response analysis of the interval/random variables models of the air spring system, a new unified orthogonal polynomial expansion method, named as sparse quadrature-based interval and random moment arbitrary polynomial chaos method (SQ-IRMAPC), is proposed. In SQ-IRMAPC, the response of the acoustic system related to both interval and random variables is approximated by the moment-based arbitrary orthogonal polynomial expansion. To efficiently calculate the coefficient of the interval and random orthogonal polynomial expansion, the sparse quadrature is introduced. The proposed SQ-IRMAPC was employed to analyze the mechanic performance of an air spring with interval and/or random variables, and its effectiveness has been demonstrated by fully comparing it with the most recently proposed orthogonal polynomial-based interval and random analysis method.

Theoretical and Experimental Research of Viscoelastic Damping Limb-Like-Structure Device with Coupling Nonlinear Characteristics

The nonlinear characteristic of vibration control systems has attracted increasing attention for its advantage in improving structural performance. In this paper, a new type of viscoelastic damping limb-like-structure (VE-LLS) device is proposed by combing the viscoelastic (VE) damper and limb-like-structure (LLS) together, which possesses coupling nonlinearity characteristic caused by geometric and material factors, as well as a remarkable advantage in improving the control performance. First, to explore the nonlinear geometrical effects on the static stiffness of the VE-LLS device, a formula is derived from static stiffness, and the results are discussed. Second, dynamic analysis is performed of the proposed device considering the coupling geometrical and material nonlinearities in frequency domain, with the real-time effect of frequency and temperature on the mechanical properties of the viscoelastic damper considered in solving the nonlinear vibration equation. The harmonic balance method (HBM) is used to solve the nonlinear dynamic equation. Then, the displacement transmissibility of the VE-LLS device is calculated and assessed. The results indicate that the proposed device possesses excellent vibration isolation performance, and the geometric parameters of the viscoelastic damper have significant nonlinear effect on the performance. Finally, an experiment is carried out of the VE-LLS device to verify the accuracy of the static stiffness analysis. The results show that the theoretical results agree well the experimental ones, and that the theoretical results have high accuracy and reliability.